Mass: 18.015 Da / Num. of mol.: 507 / Source method: isolated from a natural source / Formula: H2O
Sequence details
DUE TO THE UNUSUAL LENGTH OF THE CDR H3 LOOP, ADDITIONAL CDR H3 RESIDUES BEYOND KABAT POSITION 100Z ...DUE TO THE UNUSUAL LENGTH OF THE CDR H3 LOOP, ADDITIONAL CDR H3 RESIDUES BEYOND KABAT POSITION 100Z WERE ACCOMMODATED AT POSITIONS 101 AND 102. THESE SEQUENCES REFER TO ANTIBODY CHAINS
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.35 Å3/Da / Density % sol: 47.6 %
Crystal grow
Temperature: 295 K / Method: vapor diffusion, sitting drop Details: 0.2 M di-sodium tartrate and 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Monochromator: Side scattering bent cube-root I-beam single crystal Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 0.98 Å / Relative weight: 1
Reflection
Resolution: 2.2→50 Å / Num. obs: 49527 / % possible obs: 96.7 % / Observed criterion σ(I): -3 / Redundancy: 6.3 % / Biso Wilson estimate: 32 Å2 / Rsym value: 0.1 / Net I/σ(I): 17.8
Reflection shell
Resolution: 2.2→2.28 Å / Redundancy: 3.5 % / Mean I/σ(I) obs: 2.3 / Rsym value: 0.45 / % possible all: 75.4
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Processing
Software
Name
Version
Classification
Blu-Ice
datacollection
PHASER
phasing
PHENIX
(phenix.refine: 1.8.2_1309)
refinement
HKL-2000
datareduction
HKL-2000
datascaling
Refinement
Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4K3D