- PDB-4jso: The X-ray crystal structure of a thermophilic cellobiose binding ... -
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基本情報
登録情報
データベース: PDB / ID: 4jso
タイトル
The X-ray crystal structure of a thermophilic cellobiose binding protein bound with laminaripentaose
要素
Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein
キーワード
SUGAR BINDING PROTEIN / PERIPLASMIC BINDING PROTEIN / THERMOPHILIC PROTEIN / CELLULOSE / CELLOPENTAOSE / LAMINARIBIOSE / LAMINARPENTAOSE BINDING PROTEIN
機能・相同性
機能・相同性情報
peptide transport / peptide transmembrane transporter activity / outer membrane-bounded periplasmic space / metal ion binding 類似検索 - 分子機能
Dipeptide-binding Protein; domain 1 / Dipeptide-binding Protein; Domain 1 / Dipeptide-binding Protein; domain 3 / Dipeptide-binding Protein; Domain 3 / Solute-binding protein family 5 domain / Solute-binding protein family 5 / Bacterial extracellular solute-binding proteins, family 5 Middle / Periplasmic binding protein-like II / D-Maltodextrin-Binding Protein; domain 2 / Roll ...Dipeptide-binding Protein; domain 1 / Dipeptide-binding Protein; Domain 1 / Dipeptide-binding Protein; domain 3 / Dipeptide-binding Protein; Domain 3 / Solute-binding protein family 5 domain / Solute-binding protein family 5 / Bacterial extracellular solute-binding proteins, family 5 Middle / Periplasmic binding protein-like II / D-Maltodextrin-Binding Protein; domain 2 / Roll / Alpha-Beta Complex / 3-Layer(aba) Sandwich / Alpha Beta 類似検索 - ドメイン・相同性
Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein 類似検索 - 構成要素
温度: 293 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 8 詳細: tmCBP was concentrated to 20 mg/mL and dialyzed into 10 mM Tris, 40 mM NaCl, 0.5 mM TCEP for crystallization. Laminaripentaose was added to a final concentration of 1 mM prior to ...詳細: tmCBP was concentrated to 20 mg/mL and dialyzed into 10 mM Tris, 40 mM NaCl, 0.5 mM TCEP for crystallization. Laminaripentaose was added to a final concentration of 1 mM prior to crystallization trials. Crystals were grown in drops containing 2 uL of the protein solution mixed with 2 uL of 0.2-0.3 M magnesium acetate or calcium acetate, 20-30% (wt/vol) PEG 3350 equilibrated against 900 uL of the same solution, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K