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Open data
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Basic information
Entry | Database: PDB / ID: 4jox | ||||||
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Title | Crystal structure of cry34Ab1 protein at 2.15 A resolution | ||||||
![]() | 13.6 kDa insecticidal crystal protein | ||||||
![]() | TOXIN / beta-sandwich | ||||||
Function / homology | ![]() hemolysis by symbiont of host erythrocytes / sporulation resulting in formation of a cellular spore / : / toxin activity Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Yang, C. / Pflugrath, J.W. / Kelker, M.S. / Evan, S.L. / Narva, K.E. | ||||||
![]() | ![]() Title: Structural and biophysical characterization of Cry34Ab1 and Cry35Ab1. Authors: Kelker, M.S. / Berry, C. / Evans, S.L. / Pai, R. / McCaskill, D. / Pflugrath, J.W. / Wade, M. / Wess, T.J. / Baker, M.D. / Yang, C. / Narva, K.E. / McCaskill, D.G. / Wang, N.X. / Russell, J.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 60.1 KB | Display | ![]() |
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PDB format | ![]() | 45.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 421.9 KB | Display | ![]() |
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Full document | ![]() | 425.7 KB | Display | |
Data in XML | ![]() | 7.8 KB | Display | |
Data in CIF | ![]() | 10 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 13617.727 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.61 Å3/Da / Density % sol: 52.84 % |
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Crystal grow | Temperature: 289 K / Method: liquid diffusion / pH: 4.4 Details: 25% (V/V) PEG400, 50mM sodium acetate, pH 4.4, LIQUID DIFFUSION, temperature 289K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: Jan 1, 2002 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 2.15→50 Å / Num. all: 299280 / Num. obs: 14955 / % possible obs: 99.8 % / Observed criterion σ(F): 3 / Observed criterion σ(I): 3 / Redundancy: 19.5 % / Rmerge(I) obs: 0.091 / Net I/σ(I): 17.7 |
Reflection shell | Resolution: 2.15→2.23 Å / Redundancy: 19.5 % / Rmerge(I) obs: 0.291 / Mean I/σ(I) obs: 3.5 / % possible all: 99.6 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 45.528 Å2
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Refinement step | Cycle: LAST / Resolution: 2.15→12 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.15→2.204 Å / Total num. of bins used: 20 /
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Refinement TLS params. | Method: refined / Origin x: -8.452 Å / Origin y: 22.2251 Å / Origin z: -10.0253 Å
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