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Yorodumi- PDB-4j5y: Crystal structure of Hfq from Pseudomonas aeruginosa in complex w... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4j5y | ||||||
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Title | Crystal structure of Hfq from Pseudomonas aeruginosa in complex with ATP | ||||||
Components | Protein hfq | ||||||
Keywords | RNA BINDING PROTEIN / LSM / RNA chaperone / sRNA / mRNA | ||||||
Function / homology | Function and homology information regulation of carbon utilization / regulation of translation, ncRNA-mediated / regulation of RNA stability / quorum sensing / regulation of translation / regulation of DNA-templated transcription / RNA binding / cytosol Similarity search - Function | ||||||
Biological species | Pseudomonas aeruginosa (bacteria) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.0953 Å | ||||||
Authors | Murina, V. / Lekontseva, N. / Nikulin, A. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 2013 Title: Hfq binds ribonucleotides in three different RNA-binding sites. Authors: Murina, V. / Lekontseva, N. / Nikulin, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4j5y.cif.gz | 105.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4j5y.ent.gz | 81.1 KB | Display | PDB format |
PDBx/mmJSON format | 4j5y.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/j5/4j5y ftp://data.pdbj.org/pub/pdb/validation_reports/j5/4j5y | HTTPS FTP |
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-Related structure data
Related structure data | 3quiC 4j6wC 4j6xC 4j6yC 1u1tS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 9114.487 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) / Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228 / Gene: hfq, PA4944 / Plasmid: PET22B / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q9HUM0 #2: Chemical | ChemComp-ATP / #3: Chemical | #4: Chemical | ChemComp-PEG / | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.23 Å3/Da / Density % sol: 44.75 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 200 MM ammonium sulphate, 200 MM NaCl, 50 MM TRIS-HCL, PH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: BRUKER AXS MICROSTAR / Wavelength: 1.5418 Å |
Detector | Type: Bruker Platinum 135 / Detector: CCD / Date: Apr 22, 2012 / Details: helios |
Radiation | Monochromator: mirror / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.095→20 Å / Num. all: 28097 / Num. obs: 28097 / % possible obs: 95.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4 % / Biso Wilson estimate: 22.7 Å2 / Rmerge(I) obs: 0.091 / Net I/σ(I): 13.02 |
Reflection shell | Resolution: 2.095→2.21 Å / Redundancy: 3.6 % / Rmerge(I) obs: 0.339 / Mean I/σ(I) obs: 3.33 / Num. unique all: 3877 / % possible all: 88.44 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1U1T Resolution: 2.0953→19.574 Å / SU ML: 0.27 / Cross valid method: THROUGHOUT / Phase error: 23.03 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.0953→19.574 Å
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Refine LS restraints |
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LS refinement shell |
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