Mass: 18.015 Da / Num. of mol.: 572 / Source method: isolated from a natural source / Formula: H2O
Nonpolymer details
THE CRYSTAL WAS SOAKED IN THE PRESENCE OF ATP BUT THE ELECTRON DENSITY FITS BEST WITH ADP ...THE CRYSTAL WAS SOAKED IN THE PRESENCE OF ATP BUT THE ELECTRON DENSITY FITS BEST WITH ADP PRESUMABLY DUE TO HYDROLYSIS OF ATP BY THE KINASE.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.33 Å3/Da / Density % sol: 47.14 %
Crystal grow
Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 100mM HEPES, 200mM MgCl2, 25% PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K