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- PDB-4ilr: Recognition and Cleavage of a non-structured CRISPR RNA by its Pr... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4ilr | ||||||
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Title | Recognition and Cleavage of a non-structured CRISPR RNA by its Processing Endoribonuclease Cas6 | ||||||
![]() | CRISPR-associated endoribonuclease Cas6 2 | ||||||
![]() | HYDROLASE / RRM / RNA cleavage / RNA | ||||||
Function / homology | ![]() endonuclease activity / defense response to virus / Hydrolases; Acting on ester bonds / RNA binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Shao, Y. / Li, H. | ||||||
![]() | ![]() Title: Recognition and cleavage of a nonstructured CRISPR RNA by its processing endoribonuclease Cas6. Authors: Shao, Y. / Li, H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 66.3 KB | Display | ![]() |
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PDB format | ![]() | 49.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 424.2 KB | Display | ![]() |
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Full document | ![]() | 431.1 KB | Display | |
Data in XML | ![]() | 12.5 KB | Display | |
Data in CIF | ![]() | 15.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 32684.143 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: Q97WV8, Hydrolases; Acting on ester bonds |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.54 Å3/Da / Density % sol: 65.28 % |
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Crystal grow | Temperature: 303 K / Method: vapor diffusion, hanging drop / pH: 7.6 Details: 200mM MgCl2, 100mM Tris-Cl (pH 7.6), 32% PEG 400., VAPOR DIFFUSION, HANGING DROP, temperature 303K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR scanner 300 mm plate / Detector: IMAGE PLATE / Date: Apr 21, 2012 |
Radiation | Monochromator: Si crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9792 Å / Relative weight: 1 |
Reflection | Resolution: 3→34 Å / Num. all: 10085 / Num. obs: 8986 / % possible obs: 89.1 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 28.2 % / Biso Wilson estimate: 50 Å2 / Rsym value: 0.084 / Net I/σ(I): 42.8 |
Reflection shell | Resolution: 3→3.1 Å / Redundancy: 15.6 % / Mean I/σ(I) obs: 3.5 / Num. unique all: 500 / Rsym value: 0.427 / % possible all: 34.2 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.088→26.772 Å
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Refine LS restraints |
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LS refinement shell |
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