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- PDB-4ihf: Chasing Acyl Carrier Protein Through a Catalytic Cycle of Lipid A... -

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Basic information

Entry
Database: PDB / ID: 4ihf
TitleChasing Acyl Carrier Protein Through a Catalytic Cycle of Lipid A Production
Components
  • Acyl carrier protein
  • UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
KeywordsTRANSFERASE/LIPID BINDING PROTEIN / Acyl Carrier Protein / Left Handed Beta Helix / LpxD / acyltransferase / Lipid A / Protein-Protein complex / ACP Recognition Domain / ACP mediated product release / TRANSFERASE-LIPID BINDING PROTEIN complex
Function / homology
Function and homology information


UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase activity / UDP-3-O-(3-hydroxyacyl)glucosamine N-acyltransferase / N-acyltransferase activity / lipid A biosynthetic process / response to antibiotic / identical protein binding / cytoplasm / cytosol
Similarity search - Function
UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase LpxD / UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase, non-repeat region / UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase, LpxD / MurE/MurF, N-terminal domain / Udp-n-acetylmuramoylalanyl-d-glutamate--2,6- Diaminopimelate Ligase; Chain: A, domain 1 / Hexapeptide transferase, conserved site / Hexapeptide-repeat containing-transferases signature. / Hexapeptide repeat proteins / UDP N-Acetylglucosamine Acyltransferase; domain 1 / Hexapeptide repeat ...UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase LpxD / UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase, non-repeat region / UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase, LpxD / MurE/MurF, N-terminal domain / Udp-n-acetylmuramoylalanyl-d-glutamate--2,6- Diaminopimelate Ligase; Chain: A, domain 1 / Hexapeptide transferase, conserved site / Hexapeptide-repeat containing-transferases signature. / Hexapeptide repeat proteins / UDP N-Acetylglucosamine Acyltransferase; domain 1 / Hexapeptide repeat / Bacterial transferase hexapeptide (six repeats) / Trimeric LpxA-like superfamily / 3 Solenoid / 3-Layer(aba) Sandwich / Mainly Beta / Alpha Beta
Similarity search - Domain/homology
Chem-1F7 / : / UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
Similarity search - Component
Biological speciesEscherichia coli (E. coli)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.1 Å
AuthorsMasoudi, A. / Raetz, C.R.H. / Pemble, C.W.
CitationJournal: Nature / Year: 2014
Title: Chasing acyl carrier protein through a catalytic cycle of lipid A production.
Authors: Masoudi, A. / Raetz, C.R. / Zhou, P. / Pemble, C.W.
History
DepositionDec 18, 2012Deposition site: RCSB / Processing site: RCSB
Revision 1.0Nov 13, 2013Provider: repository / Type: Initial release
Revision 1.1Nov 20, 2013Group: Database references
Revision 1.2Jan 22, 2014Group: Database references
Revision 1.3Jan 29, 2014Group: Database references
Revision 1.4Nov 15, 2017Group: Advisory / Refinement description / Category: pdbx_unobs_or_zero_occ_atoms / software
Item: _software.classification / _software.contact_author ..._software.classification / _software.contact_author / _software.contact_author_email / _software.date / _software.language / _software.location / _software.name / _software.type / _software.version
Remark 650HELIX DETERMINATION METHOD: AUTHOR
Remark 700SHEET DETERMINATION METHOD: AUTHOR

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
B: UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
C: UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
D: UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
E: UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
F: UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
G: Acyl carrier protein
H: Acyl carrier protein
I: Acyl carrier protein
J: Acyl carrier protein
K: Acyl carrier protein
L: Acyl carrier protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)277,98820
Polymers274,40912
Non-polymers3,5798
Water16,574920
1
D: UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
E: UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
F: UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
G: Acyl carrier protein
H: Acyl carrier protein
K: Acyl carrier protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)138,99410
Polymers137,2056
Non-polymers1,7904
Water1086
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area24620 Å2
ΔGint-174 kcal/mol
Surface area48700 Å2
MethodPISA
2
A: UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
B: UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
C: UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
I: Acyl carrier protein
J: Acyl carrier protein
L: Acyl carrier protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)138,99410
Polymers137,2056
Non-polymers1,7904
Water1086
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area24650 Å2
ΔGint-175 kcal/mol
Surface area47830 Å2
MethodPISA
3


  • Idetical with deposited unit
  • defined by software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area50840 Å2
ΔGint-348 kcal/mol
Surface area94950 Å2
MethodPISA
Unit cell
Length a, b, c (Å)84.299, 89.441, 112.251
Angle α, β, γ (deg.)104.06, 92.40, 118.47
Int Tables number1
Space group name H-MP1

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Components

#1: Protein
UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase / UDP-3-O-(3-OHC14)-GlcN N-acyltransferase / Protein FirA / Rifampicin resistance protein


Mass: 36864.207 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Strain: K12 / Gene: lpxD, firA, omsA, b0179, JW0174 / Plasmid: pET11d / Production host: Escherichia coli (E. coli) / Strain (production host): C41(DE3)
References: UniProt: P21645, UDP-3-O-(3-hydroxyacyl)glucosamine N-acyltransferase
#2: Protein
Acyl carrier protein / ACP


Mass: 8870.685 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Strain: 'clone D i14' / Gene: acpP, i14_1248 / Plasmid: pET16b / Production host: Escherichia coli (E. coli) / Strain (production host): C41(DE3) / References: UniProt: G7RM21
#3: Chemical ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Cl
#4: Chemical
ChemComp-1F7 / S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] (3R)-3-hydroxytetradecanethioate


Mass: 584.703 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: C25H49N2O9PS
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 920 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.58 Å3/Da / Density % sol: 52.41 %
Crystal growTemperature: 288 K / Method: vapor diffusion / pH: 6.5
Details: 0.1 M MES pH 6.5, 0.2 M ammonium sulfate, 20% PEG 8000, vapor diffusion, temperature 288K

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Data collection

Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å
DetectorType: MARMOSAIC 300 mm CCD / Detector: CCD
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.1→50 Å / Num. all: 157708 / Num. obs: 154031 / % possible obs: 97.7 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 3.6 % / Rmerge(I) obs: 0.103 / Net I/σ(I): 8.2
Reflection shell
Resolution (Å)Redundancy (%)Rmerge(I) obsDiffraction-ID% possible all
2.1-2.143.10.402194.9
2.14-2.183.20.376196.2
2.18-2.223.30.354196.9
2.22-2.263.40.327197.1
2.26-2.313.40.288197.3
2.31-2.373.50.25197.5
2.37-2.423.60.234197.5
2.42-2.493.60.23197.7
2.49-2.563.70.208197.8
2.56-2.653.80.186198
2.65-2.743.80.169198
2.74-2.853.90.151198.2
2.85-2.983.90.128198.1
2.98-3.143.90.115198.4
3.14-3.333.90.103198.7
3.33-3.593.90.087198.6
3.59-3.953.80.077198.6
3.95-4.523.70.069197.8
4.52-5.73.80.068197.8
5.7-503.80.078198.4

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Phasing

PhasingMethod: molecular replacement

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Processing

Software
NameVersionClassificationNB
DENZOdata reduction
SCALEPACKdata scaling
PHASERphasing
PHENIX1.7.3_928refinement
PDB_EXTRACT3.11data extraction
SERGUIdata collection
HKL-2000data reduction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.1→43.38 Å / Occupancy max: 1 / Occupancy min: 0 / SU ML: 0.21 / σ(F): 0.05 / Phase error: 22.23 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.216 1809 1.24 %
Rwork0.168 --
obs0.168 145543 91.2 %
all-147352 -
Solvent computationShrinkage radii: 0.98 Å / VDW probe radii: 1.2 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 41.16 Å2 / ksol: 0.34 e/Å3
Displacement parametersBiso mean: 46.54 Å2
Baniso -1Baniso -2Baniso -3
1-4.661 Å2-10.237 Å21.448 Å2
2--0.027 Å2-0.5609 Å2
3----4.6881 Å2
Refinement stepCycle: LAST / Resolution: 2.1→43.38 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms18268 0 224 920 19412
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00718712
X-RAY DIFFRACTIONf_angle_d1.0525342
X-RAY DIFFRACTIONf_dihedral_angle_d14.6556914
X-RAY DIFFRACTIONf_chiral_restr0.0683012
X-RAY DIFFRACTIONf_plane_restr0.0043297
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.1036-2.16040.2558970.18948589X-RAY DIFFRACTION71
2.1604-2.2240.2451360.186110351X-RAY DIFFRACTION85
2.224-2.29580.24181440.182810603X-RAY DIFFRACTION88
2.2958-2.37780.22181340.173810784X-RAY DIFFRACTION89
2.3778-2.4730.22791220.171610986X-RAY DIFFRACTION90
2.473-2.58560.24521430.176811009X-RAY DIFFRACTION91
2.5856-2.72190.25751360.174511252X-RAY DIFFRACTION92
2.7219-2.89240.25461510.175911349X-RAY DIFFRACTION94
2.8924-3.11560.19441430.176611542X-RAY DIFFRACTION95
3.1156-3.42910.24561490.17511724X-RAY DIFFRACTION97
3.4291-3.9250.19431480.161211844X-RAY DIFFRACTION98
3.925-4.94390.17871500.137311822X-RAY DIFFRACTION97
4.9439-43.38550.21131560.173711879X-RAY DIFFRACTION98
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.85620.45550.42761.7109-0.03081.53060.0584-0.03520.0260.0786-0.03790.0006-0.86040.2657-0.03140.5459-0.15740.02690.249-0.00870.178530.989414.5254-15.6579
20.52340.3627-0.0070.7056-0.10721.0801-0.0769-0.00740.0884-0.06710.05280.1062-0.1071-0.142-0.00280.11930.0323-0.02040.1871-0.00580.201114.3704-3.2502-42.1677
31.39920.43230.55011.4276-0.73682.0453-0.26270.22070.1386-0.5860.31070.3624-0.1639-0.2314-0.02040.5463-0.1235-0.13250.30120.04340.2965.3756-5.3606-71.9611
40.19540.33880.21410.8733-0.07420.8227-0.02060.04080.008-0.06750.02620.10420.1758-0.23680.01690.1554-0.0302-0.00770.237-0.01290.2069.1165-21.3081-39.8682
51.31480.7308-0.42042.02430.17031.92990.0674-0.2338-0.13250.3382-0.15620.03970.2859-0.12180.07670.4234-0.1406-0.01380.25240.02040.23637.8947-46.2667-21.4196
60.18460.18390.09540.8334-0.00330.9160.0469-0.039-0.02080.1954-0.06010.03960.0368-0.10390.00240.16890.00170.01490.2057-0.00940.194616.8147-11.7676-25.4781
71.72680.44580.23781.8261-0.30691.21240.04240.15050.49990.0764-0.04830.0663-0.4560.092-0.01160.5192-0.25670.06940.3061-0.0030.344345.10523.49378.1364
80.29440.17240.14620.6237-0.09881.1575-0.0720.06660.0137-0.0860.0692-0.01880.11160.00610.0070.16210.02150.0060.15520.00120.157127.3431-6.623414.5183
90.2730.51040.00040.9501-0.02971.40.06270.0327-0.2217-0.04720.15160.12340.826-0.60350.07920.6775-0.4707-0.04840.14110.03470.215313.7934-33.00224.6288
100.53970.28180.12350.5082-0.1771.2140.0686-0.0055-0.03680.0553-0.0542-0.02570.2348-0.0959-0.00640.15750.02890.0030.11170.00220.1524.8016-12.984132.0686
111.40240.2607-0.20192.1032-0.07892.1850.0713-0.1717-0.02430.3091-0.0872-0.0114-0.0720.12220.01830.2863-0.07-0.02330.21320.02580.166630.6284-9.497662.345
120.43450.3014-0.07070.5179-0.06971.1548-0.006-0.05980.03660.0693-0.0093-0.0057-0.17310.13070.02150.16440.008-0.00840.15120.00120.183533.76563.371729.201
137.6228-3.4784-2.94212.2310.79912.8717-0.09550.2152-0.6205-0.1914-0.0623-0.05580.3562-0.18390.05890.46340.04520.15150.3122-0.12280.4144-8.64231.317651.0725
141.3575-0.2261-1.68710.6875-0.38822.8792-0.0979-0.5954-0.52190.2668-0.01670.00980.5638-0.04180.03890.5880.13370.14890.57250.09350.44182.5907-2.573453.9261
159.3642-1.8851-2.29881.98130.26692.08350.11140.2827-0.04620.1122-0.22890.0749-0.0465-0.26850.05710.43410.10560.10610.4185-0.06090.3857-0.7436.74243.5111
160.4039-0.4876-0.66610.6320.86071.17150.0871-0.05810.4761-0.00810.2855-0.225-0.46940.3517-0.15640.4615-0.05580.16180.6141-0.1360.5798-1.890412.74252.3218
173.0531-3.1599-1.29714.49642.21361.1694-0.0489-0.1105-0.00030.17190.11620.0109-0.2862-0.22540.0380.77670.2493-0.03840.6894-0.15440.73412.569836.712725.3507
182.508-0.93971.86653.0646-2.89793.28570.0158-0.01850.10790.0689-0.1171-0.2168-0.13280.32570.10210.9236-0.02190.05320.6233-0.22731.242922.34935.917721.6311
192.02611.73260.14697.201-1.5521.28460.00720.26250.5207-0.28490.0348-0.4629-0.31780.13160.00940.60250.01610.14080.4163-0.0690.826419.863425.444716.8737
207.7567-3.7861-2.13717.59431.47474.19210.0268-0.29610.39030.38370.16520.2403-0.3239-0.1324-0.03760.54240.27530.05030.3999-0.020.66558.004726.10223.041
213.08240.1932-0.78371.8726-0.13251.3804-0.07150.3566-0.2845-0.18980.05420.35640.3738-0.16550.05430.89480.2421-0.0370.35340.05390.85565.918531.487315.2217
225.2539-1.1261-2.55853.21171.27011.45180.15280.15220.32020.0806-0.19880.0041-0.2411-0.10730.06220.35890.1213-0.10440.6489-0.19940.8277-23.283412.257-48.3796
231.80890.57351.55961.25060.68831.6458-0.16280.35390.4237-0.5340.0620.2326-0.4750.15190.11770.60980.329-0.17041.00570.08060.7518-17.18919.3186-55.9915
242.8466-1.16040.66112.6189-2.30353.57050.0430.3257-0.0994-0.3953-0.24280.04840.03910.06420.12070.28450.1329-0.13460.6388-0.00660.6192-12.98080.4284-51.1409
255.3938-2.1211-0.37862.83370.28142.4368-0.1249-0.32520.13990.1238-0.00230.0292-0.1292-0.1469-0.060.16020.1750.00530.5981-0.05280.6089-20.68854.5031-41.1738
261.67450.1158-1.30411.9482-0.71232.3824-0.1078-0.2247-0.32250.2164-0.09610.22050.4538-0.23910.13260.49140.0796-0.24050.8267-0.30251.1335-27.0807-1.5275-43.8386
271.6223-0.37110.78550.5473-0.41880.66220.03970.025-0.082-0.06730.07160.1310.0235-0.1897-0.05580.33770.198-0.28180.7056-0.2440.6259-29.71873.7653-50.3844
281.31360.1753-0.76260.27320.31471.5622-0.0943-0.0003-0.062-0.0759-0.02230.31360.1019-0.1925-0.03860.8515-0.51490.24390.959-0.04771.3269-23.8365-31.3006-23.4112
290.8871.6061-0.69662.9609-1.36470.75-0.0826-0.308-0.49390.30770.06430.24660.3557-0.1121-0.00490.5838-0.18930.17520.68420.06690.7719-12.6435-28.6849-19.5359
305.4559-0.6814-0.06171.9282-0.42361.96410.05540.193-0.04880.03710.00570.45030.0177-0.42340.16850.3764-0.12830.27340.94240.07030.8079-21.6195-18.9553-21.7789
311.1714-0.1759-0.65120.6049-0.17310.48920.003-0.2164-0.03130.182-0.14140.26230.1071-0.0857-0.06520.8089-0.4720.35791.2096-0.00080.8882-23.2097-21.4356-12.4174
325.7167-2.34470.18571.347-0.58210.68830.21620.3930.1646-0.51580.14330.2484-0.2092-0.0843-0.14870.5026-0.0888-0.06070.84990.08450.4711-11.94-2.10684.9022
333.88272.6303-1.21251.8581-0.59461.12-0.0970.4441-0.0834-0.5034-0.0829-0.2240.11210.13580.11760.6316-0.0137-0.02480.9669-0.26690.5536-7.4602-9.94590.9768
343.67630.72882.49050.27210.97433.50980.08830.4073-0.5421-0.1564-0.25090.04830.4443-0.11410.05890.33380.0696-0.01440.6425-0.17370.4691-1.8717-11.018611.5116
354.0141-0.08950.05641.8171-0.3082.9437-0.2010.02580.49330.0354-0.06030.2206-0.3154-0.19220.17730.22320.0105-0.08230.5820.07750.5171-10.53840.926315.9889
361.9996-0.1083-0.5824.18112.38584.3946-0.1625-0.3564-0.12880.4963-0.05480.1930.3421-0.02270.0860.4443-0.06770.04410.6152-0.01870.6168-11.4008-9.454620.6371
374.028-0.0469-1.87190.69631.31684.7996-0.06990.1414-0.1279-0.06920.16340.2160.0268-0.2953-0.02170.19060.008-0.06290.6444-0.03520.5283-18.8425-5.982512.8503
380.2556-0.2655-0.09992.41870.51831.6124-0.0058-0.49660.27680.5761-0.0146-0.1098-0.0579-0.1274-0.02381.1994-0.15020.18150.9432-0.29020.6681-2.967712.8806-3.8312
392.0988-1.4077-0.37431.75660.90682.764-0.2138-0.2139-0.04740.5076-0.13790.3994-0.3095-0.47390.22190.58970.10660.13490.6203-0.14440.5975-4.51429.6478-14.1143
402.71920.54680.07541.36370.5351.4768-0.09190.00660.2081-0.1201-0.14130.3296-0.2055-0.5020.07191.07870.24480.22230.6953-0.11170.8701-11.437518.3256-14.4198
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'C' and (resseq 2:89)
2X-RAY DIFFRACTION2chain 'C' and (resseq 90:337)
3X-RAY DIFFRACTION3chain 'B' and (resseq 2:89)
4X-RAY DIFFRACTION4chain 'B' and (resseq 90:337)
5X-RAY DIFFRACTION5chain 'A' and (resseq 3:89)
6X-RAY DIFFRACTION6chain 'A' and (resseq 90:338)
7X-RAY DIFFRACTION7chain 'F' and (resseq 3:89)
8X-RAY DIFFRACTION8chain 'F' and (resseq 90:338)
9X-RAY DIFFRACTION9chain 'E' and (resseq 3:89)
10X-RAY DIFFRACTION10chain 'E' and (resseq 90:338)
11X-RAY DIFFRACTION11chain 'D' and (resseq 0:89)
12X-RAY DIFFRACTION12chain 'D' and (resseq 90:338)
13X-RAY DIFFRACTION13chain 'G' and (resseq 0:15)
14X-RAY DIFFRACTION14chain 'G' and (resseq 16:35)
15X-RAY DIFFRACTION15chain 'G' and (resseq 36:49)
16X-RAY DIFFRACTION16chain 'G' and (resseq 50:75)
17X-RAY DIFFRACTION17chain 'H' and (resseq 0:15)
18X-RAY DIFFRACTION18chain 'H' and (resseq 16:25)
19X-RAY DIFFRACTION19chain 'H' and (resseq 26:37)
20X-RAY DIFFRACTION20chain 'H' and (resseq 38:49)
21X-RAY DIFFRACTION21chain 'H' and (resseq 50:74)
22X-RAY DIFFRACTION22chain 'I' and (resseq 2:14)
23X-RAY DIFFRACTION23chain 'I' and (resseq 15:27)
24X-RAY DIFFRACTION24chain 'I' and (resseq 28:37)
25X-RAY DIFFRACTION25chain 'I' and (resseq 38:49)
26X-RAY DIFFRACTION26chain 'I' and (resseq 50:64)
27X-RAY DIFFRACTION27chain 'I' and (resseq 65:74)
28X-RAY DIFFRACTION28chain 'J' and (resseq 3:24)
29X-RAY DIFFRACTION29chain 'J' and (resseq 25:37)
30X-RAY DIFFRACTION30chain 'J' and (resseq 38:49)
31X-RAY DIFFRACTION31chain 'J' and (resseq 50:72)
32X-RAY DIFFRACTION32chain 'K' and (resseq 1:15)
33X-RAY DIFFRACTION33chain 'K' and (resseq 16:25)
34X-RAY DIFFRACTION34chain 'K' and (resseq 26:37)
35X-RAY DIFFRACTION35chain 'K' and (resseq 38:55)
36X-RAY DIFFRACTION36chain 'K' and (resseq 56:64)
37X-RAY DIFFRACTION37chain 'K' and (resseq 65:76)
38X-RAY DIFFRACTION38chain 'L' and (resseq 3:27)
39X-RAY DIFFRACTION39chain 'L' and (resseq 28:49)
40X-RAY DIFFRACTION40chain 'L' and (resseq 50:74)

+
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