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- PDB-4ihh: Chasing Acyl Carrier Protein Through a Catalytic Cycle of Lipid A... -

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Basic information

Entry
Database: PDB / ID: 4ihh
TitleChasing Acyl Carrier Protein Through a Catalytic Cycle of Lipid A Production
Components
  • Acyl carrier protein
  • UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
KeywordsTRANSFERASE/LIPID BINDING PROTEIN / Acyl Carrier Protein / Left Handed Beta Helix / LpxD / acyltransferase / Lipid A / Protein-Protein complex / ACP Recognition Domain / ACP mediated product release / TRANSFERASE-LIPID BINDING PROTEIN complex
Function / homology
Function and homology information


UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase activity / UDP-3-O-(3-hydroxyacyl)glucosamine N-acyltransferase / N-acyltransferase activity / lipid A biosynthetic process / response to antibiotic / identical protein binding / cytoplasm / cytosol
Similarity search - Function
UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase LpxD / UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase, non-repeat region / UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase, LpxD / MurE/MurF, N-terminal domain / Udp-n-acetylmuramoylalanyl-d-glutamate--2,6- Diaminopimelate Ligase; Chain: A, domain 1 / Hexapeptide transferase, conserved site / Hexapeptide-repeat containing-transferases signature. / Hexapeptide repeat proteins / UDP N-Acetylglucosamine Acyltransferase; domain 1 / Hexapeptide repeat ...UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase LpxD / UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase, non-repeat region / UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase, LpxD / MurE/MurF, N-terminal domain / Udp-n-acetylmuramoylalanyl-d-glutamate--2,6- Diaminopimelate Ligase; Chain: A, domain 1 / Hexapeptide transferase, conserved site / Hexapeptide-repeat containing-transferases signature. / Hexapeptide repeat proteins / UDP N-Acetylglucosamine Acyltransferase; domain 1 / Hexapeptide repeat / Bacterial transferase hexapeptide (six repeats) / Trimeric LpxA-like superfamily / 3 Solenoid / 3-Layer(aba) Sandwich / Mainly Beta / Alpha Beta
Similarity search - Domain/homology
4'-PHOSPHOPANTETHEINE / : / UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
Similarity search - Component
Biological speciesEscherichia coli (E. coli)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.132 Å
AuthorsMasoudi, A. / Raetz, C.R.H. / Pemble, C.W.
CitationJournal: Nature / Year: 2014
Title: Chasing acyl carrier protein through a catalytic cycle of lipid A production.
Authors: Masoudi, A. / Raetz, C.R. / Zhou, P. / Pemble, C.W.
History
DepositionDec 18, 2012Deposition site: RCSB / Processing site: RCSB
Revision 1.0Nov 13, 2013Provider: repository / Type: Initial release
Revision 1.1Nov 20, 2013Group: Database references
Revision 1.2Jan 22, 2014Group: Database references
Revision 1.3Jan 29, 2014Group: Database references
Revision 1.4Nov 15, 2017Group: Advisory / Refinement description / Category: pdbx_unobs_or_zero_occ_atoms / software
Item: _software.classification / _software.contact_author ..._software.classification / _software.contact_author / _software.contact_author_email / _software.date / _software.language / _software.location / _software.name / _software.type / _software.version
Remark 650HELIX DETERMINATION METHOD: AUTHOR
Remark 700SHEET DETERMINATION METHOD: AUTHOR

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
B: UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
C: UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
D: UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
E: UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
F: UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
G: Acyl carrier protein
H: Acyl carrier protein
I: Acyl carrier protein
J: Acyl carrier protein
K: Acyl carrier protein
L: Acyl carrier protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)278,32925
Polymers274,81212
Non-polymers3,51713
Water15,943885
1
A: UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
B: UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
C: UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
G: Acyl carrier protein
H: Acyl carrier protein
L: Acyl carrier protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)139,26213
Polymers137,4066
Non-polymers1,8567
Water1086
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area24660 Å2
ΔGint-183 kcal/mol
Surface area41800 Å2
MethodPISA
2
D: UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
E: UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
F: UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
I: Acyl carrier protein
J: Acyl carrier protein
K: Acyl carrier protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)139,06712
Polymers137,4066
Non-polymers1,6616
Water1086
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area24840 Å2
ΔGint-181 kcal/mol
Surface area49060 Å2
MethodPISA
Unit cell
Length a, b, c (Å)89.470, 93.225, 114.300
Angle α, β, γ (deg.)75.93, 73.81, 58.95
Int Tables number1
Space group name H-MP1

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Components

#1: Protein
UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase / UDP-3-O-(3-OHC14)-GlcN N-acyltransferase / Protein FirA / Rifampicin resistance protein


Mass: 36931.277 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Strain: K12 / Gene: lpxD, firA, omsA, b0179, JW0174 / Plasmid: pET11d / Production host: Escherichia coli (E. coli) / Strain (production host): Rosetta(DE3)/pLysS
References: UniProt: P21645, UDP-3-O-(3-hydroxyacyl)glucosamine N-acyltransferase
#2: Protein
Acyl carrier protein / ACP


Mass: 8870.685 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Strain: 'clone D i14' / Gene: acpP, i14_1248 / Plasmid: pET16b / Production host: Escherichia coli (E. coli) / Strain (production host): C41(DE3) / References: UniProt: G7RM21
#3: Chemical
ChemComp-MES / 2-(N-MORPHOLINO)-ETHANESULFONIC ACID


Mass: 195.237 Da / Num. of mol.: 7 / Source method: obtained synthetically / Formula: C6H13NO4S / Comment: pH buffer*YM
#4: Chemical
ChemComp-PNS / 4'-PHOSPHOPANTETHEINE


Mass: 358.348 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: C11H23N2O7PS
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 885 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.83 Å3/Da / Density % sol: 56.59 %
Crystal growTemperature: 288 K / Method: vapor diffusion / pH: 6
Details: 0.1 M MES pH 6.0, 0.2 M lithium sulfate, 20% PEG 4000, vapor diffusion, temperature 288K

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Data collection

Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 22-BM / Wavelength: 1 Å
DetectorType: MARMOSAIC 225 mm CCD / Detector: CCD
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.132→50 Å / Num. all: 166640 / Num. obs: 160164 / % possible obs: 96.1 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 5.4 % / Rmerge(I) obs: 0.088 / Χ2: 1.466 / Net I/σ(I): 8.7
Reflection shell
Resolution (Å)Redundancy (%)Rmerge(I) obsNum. unique allΧ2Diffraction-ID% possible all
2.15-2.1940.6471331.299185.6
2.19-2.234.20.59473171.113188
2.23-2.274.40.4675231.081190.6
2.27-2.324.50.47477461.111192.9
2.32-2.374.70.37778741.094194.3
2.37-2.424.90.32179541.117195.7
2.42-2.485.10.30780641.089196.9
2.48-2.555.30.28581301.122197.2
2.55-2.625.50.26280911.085197.6
2.62-2.715.70.20881771.116197.7
2.71-2.815.70.1981841.132198
2.81-2.925.80.14181411.192198
2.92-3.055.90.11681521.315198.2
3.05-3.215.90.182651.521198.4
3.21-3.415.90.0981761.761198.6
3.41-3.685.90.07282331.964198.8
3.68-4.055.90.0682421.995199
4.05-4.635.90.05182542.11198.9
4.63-5.835.90.04682312.058199
5.83-505.80.03682772.198199.1

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Phasing

PhasingMethod: molecular replacement

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Processing

Software
NameVersionClassificationNB
DENZOdata reduction
SCALEPACKdata scaling
PHASERphasing
PHENIX1.8.1_1168refinement
PDB_EXTRACT3.11data extraction
SERGUIdata collection
HKL-2000data reduction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.132→35.886 Å / Occupancy max: 1 / Occupancy min: 0 / FOM work R set: 0.8226 / SU ML: 0.22 / σ(F): 1.97 / Phase error: 24.94 / Stereochemistry target values: ML
RfactorNum. reflection% reflectionSelection details
Rfree0.2164 8033 5.03 %Random automatically determined by Phenix
Rwork0.1767 ---
obs0.1787 159778 95.05 %-
all-167811 --
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 136.66 Å2 / Biso mean: 38.7941 Å2 / Biso min: 3.71 Å2
Refinement stepCycle: LAST / Resolution: 2.132→35.886 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms17339 0 210 885 18434
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00717778
X-RAY DIFFRACTIONf_angle_d1.03324100
X-RAY DIFFRACTIONf_chiral_restr0.0642844
X-RAY DIFFRACTIONf_plane_restr0.0043128
X-RAY DIFFRACTIONf_dihedral_angle_d14.2836555
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 30

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.1324-2.15670.30481800.26183249342962
2.1567-2.1820.30882430.25234624486786
2.182-2.20860.28932750.23234636491188
2.2086-2.23660.25822480.22754777502589
2.2366-2.2660.25332490.22494827507692
2.266-2.29710.28142550.21845016527193
2.2971-2.32990.26672440.19724961520594
2.3299-2.36460.2392870.18585063535095
2.3646-2.40160.23232630.18185011527495
2.4016-2.44090.20692900.18645140543096
2.4409-2.4830.25152820.19285204548697
2.483-2.52820.26962400.19175130537097
2.5282-2.57680.23732540.18645228548297
2.5768-2.62940.24172660.18445183544997
2.6294-2.68650.22632740.18815132540697
2.6865-2.7490.25062790.18835203548298
2.749-2.81770.24522860.19165241552798
2.8177-2.89390.25142740.18915211548598
2.8939-2.9790.23492690.19375187545698
2.979-3.07510.23972690.19175273554298
3.0751-3.18490.22042490.18135252550198
3.1849-3.31230.20712840.18555222550698
3.3123-3.4630.21742760.17675282555898
3.463-3.64540.21083060.16815174548099
3.6454-3.87350.21132980.15995280557899
3.8735-4.17220.18452870.14425213550099
4.1722-4.59130.16012800.1345269554999
4.5913-5.25390.16973000.14555202550298
5.2539-6.61260.2142560.17465290554699
6.6126-35.89150.19672700.18055265553599
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.11960.0132-0.04860.0034-0.01210.1531-0.0747-0.04790.01050.0177-0.035-0.0410.02250.0741-0.05360.0812-0.01520.00460.3271-0.04860.150646.2362-24.737.055
20.2165-0.0010.02230.05230.02940.0292-0.0171-0.18160.070.0496-0.0136-0.0403-0.0059-0.0069-0.0080.07080.0074-0.00120.0577-0.0050.068910.2071-25.68443.7877
30.0362-0.01770.02660.0098-0.01350.01870.0258-0.0175-0.0883-0.0008-0.00050.03760.0345-0.0234-0.00430.34620.0606-0.05110.08390.0060.433816.8798-65.9059-4.8479
40.12540.01140.06350.09070.09980.2296-0.0231-0.03870.1113-0.0033-0.09360.05790.0123-0.1764-0.12510.10140.01040.00170.222-0.01610.1109-12.4141-25.81871.3738
50.096-0.0279-0.05050.05260.06350.0763-0.00510.1442-0.13490.0181-0.0054-0.0220.0263-0.0294-0.01270.0883-0.00290.00780.0701-0.03550.14496.9452-42.8913-14.4892
60.0506-0.01370.01920.0314-0.01530.06960.02930.049-0.0052-0.0640.0255-0.00330.0276-0.00650.04650.13320.00390.00650.3748-0.05410.076810.5575-39.9773-45.1172
70.22220.02330.14480.04120.03170.1716-0.01760.1803-0.0083-0.01840.0418-0.05930.01640.18480.03630.08510.00270.01590.11670.01020.079424.8229-27.0569-14.5854
8-0.0005-0.0006-0.00780.00450.01290.04270.0178-0.0325-0.06340.01230.015-0.02390.0420.02150.0160.38870.0255-0.05430.0990.04270.493515.9434-65.8464-1.062
90.01230.00110.01030.0409-0.01250.02120.0290.06270.0064-0.048-0.01170.09330.0144-0.05610.00020.12730.0178-0.00970.4060.02870.1864-65.0954-38.4623-71.6282
100.0721-0.01540.04640.00350.00070.08410.03280.16080.0515-0.07230.00430.0287-0.00050.02980.08870.13490.01110.03020.06030.03660.0969-29.4437-34.0662-68.9954
110.02570.01090.02510.0288-0.01230.03360.0172-0.0127-0.07080.04280.0149-0.01670.02580.00370.01750.3048-0.01120.02330.00590.06850.2558-32.1123-70.4475-48.8452
120.048-0.0044-0.03590.00930.02030.05630.0201-0.00510.0225-0.02020.0586-0.04730.0030.13530.08730.09450.01950.02870.4211-0.03270.0663-0.398-40.03-75.6014
130.1516-0.0702-0.04930.0359-0.01760.3333-0.0041-0.12180.036-0.01240.0265-0.09870.1350.2229-0.01690.0094-0.09550.0183-0.08080.00050.088-23.1546-38.9203-47.992
140-0.00010.0002-0.00030.00010.0109-0.0003-0.00940.01290.0319-0.00290.009-0.0001-0.0096-0.00240.08170.01110.0110.1834-0.11890.0938-31.8995-24.7416-15.4476
150.00670.0068-0.00130.02330.00010.00150.0319-0.006-0.02440.03210.0177-0.03880.06310.0110.00640.1011-0.0102-0.03770.2408-0.06990.067-25.3293-37.5771-17.6109
160.06330.00250.04470.00290.01210.19310.0239-0.08060.0681-0.01770.00510.05040.0187-0.15040.03780.08910.00520.02440.0287-0.01930.1161-43.3728-31.416-49.2026
17-0.0013-0.0011-0.00420.0020.00550.02850.01220.002-0.02360.00750.0242-0.00470.0425-0.00630.04950.35680.0373-0.00760.0220.00740.307-31.1261-71.437-52.6916
180.00110.000200.0006-0.00040.0002-0.00280.0055-0.0136-0.0134-0.004-0.0137-0.0073-0.004600.4387-0.0111-0.02610.41470.14480.4397-20.2143-77.1741-27.0635
190.00060.00060.00010.0004-0.00020.00030.00530.00020.0062-0.0040.0067-0.00450.00040.004900.7483-0.0316-0.01930.73930.07720.7738-15.6271-67.8164-25.9013
200.00040.00050.00080.00080.00010.0020.0027-0.0010.0020.0004-0.001-0.0044-0.0002-0.0024-00.7397-0.0541-0.06660.6590.00430.7127-23.3946-61.2576-25.5531
210.00060.00030.00010.0008-0.00020.00110.00630.0068-0.0062-0.00640.0029-0.00120.0052-0.0005-00.58970.0481-0.03180.4580.1110.538-29.1884-70.4997-34.1974
220.00030.000400.0008-0.00060.00090.0153-0.00820.00060.00430.00890.00550.0058-0.0081-00.82580.0130.02040.81260.04310.8282-35.252-71.9838-27.3941
230.00020.0003-0.00020.0003-0.00040.00070.006-0.0069-0.00560.00250.00590.0071-0.005-0.0023-00.6403-0.0363-0.00290.57690.07790.599-29.2411-74.2906-20.9107
240.0012-0.0007-0.00050.00120.00050.0002-0.0018-0.0026-0.0091-0.0065-0.00130.00550.0066-0.012-00.5359-0.13340.09720.48810.01820.6282-62.3344-76.4103-52.4071
250.00260.0001-0.00070.0007-0.00020.00070.00210.00080.001-0.00120.0047-0.0003-0.0032-0.0029-00.7513-0.0484-0.00240.7628-0.00810.7995-63.8825-64.3025-53.1959
260.0005-0.0002-0.00030.00010.00010.0001-0.00020.0004-0.0009-0.001-0.00030.00220.0013-0.0011-00.6159-0.052-0.01930.6263-0.0090.6797-61.1187-65.5813-63.0457
270.00430.0014-0.00020.00160.00060.00220.00640.01350.02590.00940.00080.00740.0055-0.000200.4675-0.15720.04460.25320.03710.5111-49.9584-72.9422-56.245
280.00030.0002-0.00020.00110.0010.0008-0.00140.0077-0.0025-0.0066-0.00790.00420.0062-0.0053-00.8145-0.1543-0.00180.7537-0.04960.8469-54.4284-76.372-64.7264
290.0007-0.0009-0.00020.0009-00.0014-0.00370.0028-0.0106-0.00010.0037-0.0027-0.0012-0.003900.78780.09110.00950.7283-0.03270.7066-16.556-76.689-72.9593
300.0063-0.00540.00120.00890.00010.0005-0.00030.0001-0.00020.00070.00240.0012-0.0001-0.00160.00010.92710.0180.01290.9241-0.00110.9056-15.4735-69.3661-79.5029
310.0006-0.0004-0.00070.0012-0.00040.0009-0.00130.00230.0004-0.0057-0.0024-0.0104-0.00030.0047-00.53470.06970.03560.45290.02210.4839-11.3374-66.0573-69.6843
320.00040.00010.00090.0002-00.0016-0.00630.00080.0027-0.0062-0.00170.0072-0.0036-0.0083-00.56430.0958-0.02490.3705-0.04720.492-20.3205-74.8328-63.5908
330.0002-0.00010.00080.00160.00130.0046-0.0023-0.0015-0.003-0.001-0.0013-0.00240.0001-0.0026-00.69970.0483-0.00950.6245-0.00870.6705-17.9107-82.8251-60.1381
340.0006-0-0.00040.0005-0.00040.00230.002-0.00420.00370.00340.0035-0.00690.00030.0018-00.78540.04820.01120.80370.00090.7878-9.7188-73.5213-57.7464
350.0009-0.0011-0.00050.00090.00010.0003-0.00380.0047-0.0051-0.00170.00940.00110.00230.006300.63850.16270.0630.55280.02660.6454-8.558-80.3867-65.5179
360.0011-0.0001-0.00020.0005-0.00050.0003-0.0033-0.0027-0.0007-0.00150.0043-0.0026-0.00110.0075-00.80170.0506-0.01260.8688-0.01370.9445.9618-61.3773-0.6833
370.0027-0.00250.00180.0022-0.00150.00070.0073-0.0114-0.0068-0.0018-0.00550.0018-0.00940.023500.5370.1374-0.09610.47010.01670.680238.0992-58.18324.1183
380.0006-0.000900.0010.0002-00.0025-0.0052-0.00050.01080.001-0.0043-0.0030.005300.97050.0788-0.08860.95080.0271.042537.5715-66.784110.5991
390.00020.0003-00.0014-0.00020.0004-0.0067-0.0003-0.00110.0016-0.0075-0.0007-0.0025-0.0035-00.6221-0.0609-0.01260.55270.06540.5784.0744-61.58437.7516
400.0004-0.00030.00010.00270.00050.0001-0.00340.00290.00510.0002-0.00010.0024-0.00220.0009-00.61440.038-0.01970.5988-0.07960.671614.0397-65.2682-19.7852
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 0 through 89 )A0
2X-RAY DIFFRACTION2chain 'A' and (resid 90 through 298 )A0
3X-RAY DIFFRACTION3chain 'A' and (resid 299 through 337 )A0
4X-RAY DIFFRACTION4chain 'B' and (resid 3 through 149 )B0
5X-RAY DIFFRACTION5chain 'B' and (resid 150 through 337 )B0
6X-RAY DIFFRACTION6chain 'C' and (resid 2 through 89 )C0
7X-RAY DIFFRACTION7chain 'C' and (resid 90 through 298 )C0
8X-RAY DIFFRACTION8chain 'C' and (resid 299 through 338 )C0
9X-RAY DIFFRACTION9chain 'D' and (resid 3 through 89 )D0
10X-RAY DIFFRACTION10chain 'D' and (resid 90 through 298 )D0
11X-RAY DIFFRACTION11chain 'D' and (resid 299 through 337 )D0
12X-RAY DIFFRACTION12chain 'E' and (resid -1 through 89 )E0
13X-RAY DIFFRACTION13chain 'E' and (resid 90 through 339 )E0
14X-RAY DIFFRACTION14chain 'F' and (resid 0 through 28 )F0
15X-RAY DIFFRACTION15chain 'F' and (resid 29 through 78 )F0
16X-RAY DIFFRACTION16chain 'F' and (resid 79 through 298 )F0
17X-RAY DIFFRACTION17chain 'F' and (resid 299 through 338 )F0
18X-RAY DIFFRACTION18chain 'I' and (resid 0 through 14 )I0
19X-RAY DIFFRACTION19chain 'I' and (resid 15 through 24 )I0
20X-RAY DIFFRACTION20chain 'I' and (resid 25 through 35 )I0
21X-RAY DIFFRACTION21chain 'I' and (resid 36 through 49 )I0
22X-RAY DIFFRACTION22chain 'I' and (resid 50 through 64 )I0
23X-RAY DIFFRACTION23chain 'I' and (resid 65 through 74 )I0
24X-RAY DIFFRACTION24chain 'J' and (resid -1 through 14 )J0
25X-RAY DIFFRACTION25chain 'J' and (resid 15 through 21 )J0
26X-RAY DIFFRACTION26chain 'J' and (resid 22 through 31 )J0
27X-RAY DIFFRACTION27chain 'J' and (resid 32 through 55 )J0
28X-RAY DIFFRACTION28chain 'J' and (resid 56 through 74 )J0
29X-RAY DIFFRACTION29chain 'K' and (resid 3 through 15 )K0
30X-RAY DIFFRACTION30chain 'K' and (resid 16 through 20 )K0
31X-RAY DIFFRACTION31chain 'K' and (resid 21 through 37 )K0
32X-RAY DIFFRACTION32chain 'K' and (resid 38 through 49 )K0
33X-RAY DIFFRACTION33chain 'K' and (resid 50 through 55 )K0
34X-RAY DIFFRACTION34chain 'K' and (resid 56 through 64 )K0
35X-RAY DIFFRACTION35chain 'K' and (resid 65 through 75 )K0
36X-RAY DIFFRACTION36chain 'G' and (resid 4 through 21 )G0
37X-RAY DIFFRACTION37chain 'G' and (resid 22 through 48 )G0
38X-RAY DIFFRACTION38chain 'G' and (resid 49 through 73 )G0
39X-RAY DIFFRACTION39chain 'L' and (resid 35 through 44 )L0
40X-RAY DIFFRACTION40chain 'H' and (resid 35 through 44 )H0

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