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- PDB-4ic9: Crystal structure of the full-length matrix subunit (p15) of the ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4ic9 | ||||||
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Title | Crystal structure of the full-length matrix subunit (p15) of the Feline Immunodeficiency Virus (FIV) Gag polyprotein | ||||||
![]() | Matrix protein p15 | ||||||
![]() | VIRAL PROTEIN / FIV / p15 / Retroviral matrix protein | ||||||
Function / homology | ![]() viral budding via host ESCRT complex / viral nucleocapsid / nucleic acid binding / structural constituent of virion / zinc ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Serriere, J. / Robert, X. / Perez, M. / Gouet, P. / Guillon, C. | ||||||
![]() | ![]() Title: Biophysical characterization and crystal structure of the Feline Immunodeficiency Virus p15 matrix protein. Authors: Serriere, J. / Robert, X. / Perez, M. / Gouet, P. / Guillon, C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 37.8 KB | Display | ![]() |
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PDB format | ![]() | 25.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 436.6 KB | Display | ![]() |
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Full document | ![]() | 437 KB | Display | |
Data in XML | ![]() | 7.4 KB | Display | |
Data in CIF | ![]() | 9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4icaC ![]() 1ecwS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 16358.727 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||
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#2: Chemical | ChemComp-EDO / #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal grow | Temperature: 292 K / Method: vapor diffusion, sitting drop / pH: 6 Details: 0.1 M sodium acetate, 25% (w/v) polyethylene glycol 3,000, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Feb 23, 2012 |
Radiation | Monochromator: Kirkpatrick-Baez pair of bi-morph mirrors + channel-cut cryo-cooled silicon monochromator Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98011 Å / Relative weight: 1 |
Reflection | Resolution: 2→20 Å / Num. obs: 7647 / % possible obs: 99.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 6.9 % |
Reflection shell | Resolution: 2→2.05 Å / Mean I/σ(I) obs: 4.78 / Num. unique all: 544 / % possible all: 99.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 1ECW, SIV matrix protein Resolution: 2→19.31 Å / SU ML: 0.21 / σ(F): 2.01 / Phase error: 24.85 / Stereochemistry target values: Engh & Huber
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→19.31 Å
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Refine LS restraints |
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LS refinement shell |
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