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- PDB-4h60: High resolution structure of Vibrio cholerae chemotaxis protein C... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4h60 | ||||||
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Title | High resolution structure of Vibrio cholerae chemotaxis protein CheY4 crystallized in low pH (4.0) condition | ||||||
![]() | Chemotaxis protein CheY | ||||||
![]() | SIGNALING PROTEIN / Rossmann Fold / response regulator / chemotaxis | ||||||
Function / homology | Response regulator / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta / : ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Biswas, M. / Dasgupta, J. / Sen, U. | ||||||
![]() | ![]() Title: Conformational Barrier of CheY3 and Inability of CheY4 to Bind FliM Control the Flagellar Motor Action in Vibrio cholerae. Authors: Biswas, M. / Dey, S. / Khamrui, S. / Sen, U. / Dasgupta, J. #1: Journal: Acta Crystallogr.,Sect.F / Year: 2011 Title: Overexpression, purification, crystallization and preliminary X-ray analysis of CheY4 from Vibrio cholerae O395. Authors: Biswas, M. / Khamrui, S. / Sen, U. / Dasgupta, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 40.2 KB | Display | ![]() |
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PDB format | ![]() | 26.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 3to5SC ![]() 4hnqC ![]() 4hnrC ![]() 4hnsC ![]() 4jp1C ![]() 4lx8C S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 13290.412 Da / Num. of mol.: 1 / Fragment: UNP residues 7-125 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-CA / |
#3: Chemical | ChemComp-SO4 / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.81 Å3/Da / Density % sol: 31.96 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 4 Details: 1.6 M ammonium sulfate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Jan 1, 2004 |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 1.66→30 Å / Num. all: 11447 / Num. obs: 10882 / % possible obs: 95.1 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Biso Wilson estimate: 18.4 Å2 / Rmerge(I) obs: 0.0449 / Net I/σ(I): 8.6 |
Reflection shell | Highest resolution: 1.66 Å |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 3TO5 Resolution: 1.66→22.24 Å / Rfactor Rfree error: 0.01 / Data cutoff high absF: 861015.34 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber / Details: BULK SOLVENT MODEL USED
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 44.7319 Å2 / ksol: 0.4 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 20.3 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.66→22.24 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.66→1.76 Å / Rfactor Rfree error: 0.032 / Total num. of bins used: 6
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Xplor file |
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