ソフトウェア | 名称 | バージョン | 分類 | NB |
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MOSFLM | | データ削減 | | SCALA | 3.3.16データスケーリング | | BUSTER-TNT | BUSTER 2.8.0精密化 | | PDB_EXTRACT | 3.11 | データ抽出 | | Blu-Ice | Epicsデータ収集 | | PHASER | | 位相決定 | | BUSTER | 2.1 | 精密化 | | | | |
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精密化 | 構造決定の手法: 分子置換 開始モデル: PDB ENTRY 3LYF 解像度: 3.1→46.39 Å / Cor.coef. Fo:Fc: 0.9092 / Cor.coef. Fo:Fc free: 0.8577 / Occupancy max: 1 / Occupancy min: 1 / 交差検証法: THROUGHOUT / σ(F): 0 / 立体化学のターゲット値: Engh & Huber
| Rfactor | 反射数 | %反射 | Selection details |
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Rfree | 0.2558 | 1617 | 5.06 % | RANDOM |
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Rwork | 0.2155 | - | - | - |
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obs | 0.2175 | 31959 | - | - |
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all | - | 32046 | - | - |
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原子変位パラメータ | Biso max: 300 Å2 / Biso mean: 122.4484 Å2 / Biso min: 3.41 Å2
| Baniso -1 | Baniso -2 | Baniso -3 |
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1- | 4.1508 Å2 | 0 Å2 | 0 Å2 |
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2- | - | 4.1508 Å2 | 0 Å2 |
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3- | - | - | -8.3015 Å2 |
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Refine analyze | Luzzati coordinate error obs: 0.737 Å |
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精密化ステップ | サイクル: LAST / 解像度: 3.1→46.39 Å
| タンパク質 | 核酸 | リガンド | 溶媒 | 全体 |
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原子数 | 11404 | 0 | 5 | 0 | 11409 |
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拘束条件 | Refine-ID | タイプ | 数 | Restraint function | Weight | Dev ideal |
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X-RAY DIFFRACTION | t_dihedral_angle_d5515 | SINUSOIDAL2 | | X-RAY DIFFRACTION | t_trig_c_planes281 | HARMONIC2 | | X-RAY DIFFRACTION | t_gen_planes1696 | HARMONIC5 | | X-RAY DIFFRACTION | t_it11629 | HARMONIC20 | | X-RAY DIFFRACTION | t_nbd | | | | X-RAY DIFFRACTION | t_improper_torsion | | | | X-RAY DIFFRACTION | t_pseud_angle | | | | X-RAY DIFFRACTION | t_chiral_improper_torsion1487 | SEMIHARMONIC5 | | X-RAY DIFFRACTION | t_sum_occupancies | | | | X-RAY DIFFRACTION | t_utility_distance | | | | X-RAY DIFFRACTION | t_utility_angle | | | | X-RAY DIFFRACTION | t_utility_torsion | | | | X-RAY DIFFRACTION | t_ideal_dist_contact13825 | SEMIHARMONIC4 | | X-RAY DIFFRACTION | t_bond_d11629 | HARMONIC2 | 0.009 | X-RAY DIFFRACTION | t_angle_deg15694 | HARMONIC2 | 1.02 | X-RAY DIFFRACTION | t_omega_torsion | | | 2.22 | X-RAY DIFFRACTION | t_other_torsion | | | 3.14 | | | | | | | | | | | | | | | | | | | | | | | | | |
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LS精密化 シェル | 解像度: 3.1→3.2 Å / Total num. of bins used: 16
| Rfactor | 反射数 | %反射 |
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Rfree | 0.2872 | 143 | 4.98 % |
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Rwork | 0.2561 | 2728 | - |
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all | 0.2576 | 2871 | - |
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精密化 TLS | 手法: refined / Refine-ID: X-RAY DIFFRACTION ID | L11 (°2) | L12 (°2) | L13 (°2) | L22 (°2) | L23 (°2) | L33 (°2) | S11 (Å °) | S12 (Å °) | S13 (Å °) | S21 (Å °) | S22 (Å °) | S23 (Å °) | S31 (Å °) | S32 (Å °) | S33 (Å °) | T11 (Å2) | T12 (Å2) | T13 (Å2) | T22 (Å2) | T23 (Å2) | T33 (Å2) | Origin x (Å) | Origin y (Å) | Origin z (Å) |
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1 | 1.079 | -2.7207 | 3.0152 | 7.547 | -4.9628 | 2.4158 | 0.1629 | 0.5668 | 0.3854 | -0.0909 | -0.4578 | -0.8148 | -0.2523 | 0.0527 | 0.2949 | -0.3291 | 0.0469 | 0.0873 | -0.4626 | -0.0266 | -0.3083 | 67.5173 | 25.1234 | -25.544 | 2 | 0.171 | 0.2699 | -0.4448 | 0.2547 | 0.0082 | 0 | -0.1647 | -0.0641 | -0.0092 | 0.1572 | -0.126 | -0.2483 | 0.1718 | -0.2252 | 0.2907 | -0.1319 | 0.0886 | -0.0381 | -0.273 | 0.0553 | -0.3243 | 45.123 | 38.873 | -22.8233 | 3 | 1.5803 | -1.0667 | -0.0342 | -1.0456 | -0.2368 | 0 | 0.0137 | 0.0701 | 0.1243 | 0.1051 | 0.2043 | -0.1883 | 0.175 | 0.0616 | -0.218 | 0.4559 | 0.041 | -0.1438 | 0.114 | 0.1454 | -0.0365 | 51.9039 | 31.2415 | -11.8804 | 4 | 2.2733 | -1.0293 | -0.1109 | -1.9463 | 1.6148 | 1.202 | -0.0853 | -0.2906 | -0.234 | -0.643 | 0.3387 | 0.7919 | 0.2448 | 0.3839 | -0.2535 | -0.2232 | -0.1256 | 0.0382 | -0.3507 | 0.0997 | -0.2716 | 57.827 | -23.9673 | -24.7014 | 5 | 0.3297 | -0.1754 | -0.9003 | 1.2435 | 0.1281 | 1.5667 | 0.1587 | -0.0315 | 0.1842 | -0.2104 | -0.0627 | -0.3057 | 0.013 | -0.1113 | -0.0961 | -0.1935 | 0.0347 | 0.0131 | -0.4511 | 0.035 | -0.3887 | 65.7744 | 10.4712 | -24.8982 | 6 | -3.3497 | 1.7656 | 0.0014 | 3.933 | 2.6554 | 0.6028 | -0.1339 | 0.1089 | -0.6138 | 0.1936 | 0.2917 | 0.1516 | -0.4831 | -0.2163 | -0.1578 | 0.1974 | -0.0968 | 0.0425 | 0.1664 | -0.1097 | 0.1852 | 56.4229 | 1.2343 | -11.0118 | 7 | 1.1769 | -1.3618 | 0.3208 | 1.1867 | 1.8054 | 1.8857 | 0.1927 | 0.1218 | 0.1386 | 0.1525 | -0.0928 | -0.1066 | 0.435 | -0.4003 | -0.0999 | 0.5154 | -0.1019 | -0.0329 | 0.2946 | 0.0189 | 0.1544 | 60.3368 | -1.2279 | -11.8721 | 8 | 1.7121 | -3.4855 | 1.2118 | 12.5383 | 2.7835 | 12.2922 | 0.198 | 0.057 | 0.3825 | -0.0912 | -0.4421 | -0.8475 | -0.3535 | -0.1117 | 0.2441 | 0.6847 | -0.1672 | -0.2457 | 0.1765 | -0.0502 | 0.1796 | 57.8586 | 50.1415 | -22.5209 | 9 | 2.7145 | -1.8787 | -1.5892 | -1.6355 | -7.5897 | 0 | -0.6183 | 0.1208 | 0.0558 | 0.5529 | 0.958 | 0.0112 | -0.4868 | -0.2789 | -0.3397 | -0.1307 | 0.1496 | 0.0716 | -0.2614 | 0.0006 | -0.1731 | 33.8646 | 49.443 | -25.803 | 10 | 0.9281 | 2.0656 | 2.2211 | 2.0758 | 0.7437 | 0.7095 | -0.0623 | -0.1205 | -0.0949 | -0.1392 | -0.0128 | 0.0385 | 0.0178 | -0.022 | 0.0752 | -0.2214 | -0.0519 | 0.1346 | -0.3167 | 0.0915 | -0.2584 | 8.3805 | 40.8958 | -36.1947 | 11 | 15.541 | 3.3376 | -5.1101 | -1.3264 | -2.7907 | 0 | -0.1386 | -0.2695 | 0.8494 | 0.3109 | -0.2326 | 0.7007 | 0.1445 | -0.748 | 0.3712 | 0.2752 | -0.1416 | 0.2434 | -0.2001 | 0.0161 | -0.2772 | 6.4276 | 41.2195 | -19.5146 | 12 | -0.8105 | 0.4679 | 0.2594 | 0.8105 | -2.0476 | 0 | -0.1631 | -0.2415 | 0.3574 | 0.0763 | -0.1321 | 0.3161 | 0.4069 | 0.2337 | 0.2953 | 0.0218 | 0.0899 | 0.1826 | -0.3836 | 0.0877 | -0.6079 | 17.0801 | 37.6097 | -14.1486 | 13 | 1.0826 | -0.2263 | -1.318 | -0.6221 | -4.8406 | 1.9687 | 0.2819 | 0.2143 | -0.263 | -0.0816 | -0.6654 | 0.2917 | 0.2217 | 0.3291 | 0.3835 | 0.5545 | 0.0296 | -0.0081 | 0.3787 | -0.0741 | 0.2875 | 21.9678 | 42.635 | -16.9359 | 14 | -1.2463 | -1.4191 | -0.9485 | 8.7716 | 2.6194 | 7.9741 | 0.347 | -0.5506 | 0.088 | 0.1806 | -0.6099 | -0.0803 | -0.4341 | 0.1477 | 0.2629 | 0.8676 | -0.1458 | 0.136 | 0.334 | 0.1741 | -0.4843 | 21.6854 | 34.7005 | -5.6104 | 15 | 7.3181 | -1.4278 | 6.9352 | -4.0881 | -2.6706 | 0 | -0.0297 | 0.3927 | -0.0083 | -0.1877 | -0.3217 | 1.1587 | 0.673 | -0.4396 | 0.3513 | 0.6283 | -0.1635 | 0.1935 | 0.1283 | 0.0392 | 0.03 | 1.681 | -31.7564 | -22.8076 | 16 | 4.9482 | 0.8239 | -4.6839 | 0.4068 | -2.5049 | 0 | -0.1727 | -0.3751 | -0.7343 | -0.3328 | -0.0531 | -0.0933 | -0.1016 | -0.1355 | 0.2258 | -0.3496 | -0.0356 | -0.0776 | -0.2348 | -0.1424 | -0.3626 | 41.2833 | -29.1882 | -29.5068 | 17 | -1.6939 | 0.9838 | -1.8808 | 1.7006 | 2.1389 | 0 | -0.0958 | -0.0262 | -0.1262 | -0.4123 | 0.0502 | -0.1367 | -0.275 | -0.0401 | 0.0456 | -0.185 | 0.0053 | 0.0239 | -0.4113 | -0.0085 | -0.5982 | 51.4667 | -16.6164 | -36.5605 | 18 | 0.2509 | 0.5072 | -0.199 | 0.5021 | -0.3553 | 2.188 | -0.0569 | -0.2407 | -0.3408 | -0.3591 | -0.188 | -0.0817 | -0.2644 | 0.2209 | 0.2448 | -0.1299 | -0.1177 | -0.0218 | 0.1124 | -0.0188 | -0.1457 | 44.1663 | -21.2175 | -14.4304 | 19 | 8.8709 | -1.5142 | 1.9163 | -2.6497 | 7.8277 | 0.8207 | -0.127 | 0.6336 | 0.9896 | -0.4634 | 0.1164 | 0.0571 | -1.2257 | 0.2166 | 0.0106 | 0.2763 | -0.0719 | -0.1093 | 0.1829 | 0.1718 | 0.1491 | 32.6891 | -7.9908 | -17.2052 | 20 | 1.7309 | 0.6566 | -1.0212 | 0.1812 | 0.1168 | 0 | 0.0942 | -0.6689 | -0.1866 | -0.283 | -0.1339 | 0.0992 | -0.2801 | -0.0283 | 0.0397 | 0.2539 | -0.0747 | -0.0356 | 0.5879 | 0.0513 | 0.1648 | 38.9997 | -29.1008 | -11.5533 | 21 | 7.6251 | -7.479 | 1.9806 | 3.0327 | 2.8824 | 2.755 | 0.3659 | -0.7322 | -0.7435 | 1.0496 | -0.0457 | 0.1955 | 0.5642 | -0.048 | -0.3202 | 0.4438 | -0.2875 | 0.3142 | 0.2297 | 0.0637 | 0.6046 | -10.6126 | -2.3078 | -23.191 | 22 | 0.3032 | -0.2642 | -0.3857 | 0.0057 | 1.5502 | 1.5867 | -0.005 | 0.1166 | 0.2616 | 0.245 | -0.0413 | -0.1831 | 0.0683 | 0.0239 | 0.0464 | -0.0216 | 0.0339 | -0.1925 | -0.3346 | -0.033 | -0.0392 | 11.362 | -18.7763 | -20.7242 | 23 | 1.3732 | -0.7944 | -0.5666 | -1.3678 | -4.3141 | 1.5293 | 0.0955 | -0.1334 | 0.1617 | -0.3579 | 0.0554 | -0.0398 | 0.0865 | -0.4069 | -0.1509 | 0.5466 | -0.1356 | 0.1976 | 0.2081 | 0.1629 | 0.2552 | 0.0558 | 40.2442 | -25.6158 | 24 | -2.2589 | 1.3042 | 2.1767 | 2.2589 | 1.2263 | 0.2012 | 0.4725 | 0.2546 | 0.224 | -0.1044 | -0.3485 | -0.3748 | -0.2459 | 0.231 | -0.124 | -0.4945 | -0.0744 | 0.2825 | -0.6906 | 0.1186 | 0.0593 | -8.5026 | 5.9766 | -34.216 | 25 | 3.619 | -2.9314 | 7.4211 | -0.7026 | -1.2978 | 0 | 0.1373 | -0.4525 | -0.2226 | 0.4788 | -0.2794 | 0.106 | 0.2675 | -0.1404 | 0.1421 | -0.2733 | -0.2663 | 0.3748 | -0.6209 | -0.1346 | 0.3645 | -6.992 | 7.9382 | -21.1248 | 26 | -0.6756 | 0.5397 | 3.7823 | 0.8484 | -3.1232 | 0 | -0.0378 | 0.1545 | -0.1266 | -0.033 | -0.0922 | -0.0452 | -0.0397 | 0.1304 | 0.13 | -0.3888 | -0.1762 | 0.2108 | -0.5175 | 0.2429 | 0.0274 | 4.2703 | 2.3002 | -13.394 | 27 | -3.2027 | 1.9745 | 3.353 | 3.3475 | 5.1823 | 0 | 0.1402 | -0.3816 | -0.3226 | 0.5773 | -0.0136 | -0.1549 | 0.8527 | 0.1394 | -0.1266 | -0.263 | -0.2745 | 0.2344 | -0.648 | -0.2156 | -0.3471 | -2.529 | 15.2675 | -12.5215 | 28 | 10.5649 | 4.3156 | -2.677 | 12.1581 | -1.533 | 16.5147 | -0.0452 | 0.1392 | 0.4671 | 0.3855 | 0.3217 | -0.4372 | -0.6378 | 0.8882 | -0.2765 | 0.4962 | -0.3685 | -0.4152 | 0.2197 | 0.1113 | 0.6079 | 5.2228 | 16.2494 | -13.5028 | 29 | 5.2776 | 6.2384 | 6.0188 | 5.4443 | 0.1528 | 5.6652 | 0.0161 | 0.5222 | -0.1874 | -0.4016 | -0.1448 | -0.3541 | 0.236 | 0.7286 | 0.1287 | 0.3343 | 0.146 | 0.1925 | 0.8677 | 0.1771 | 0.2771 | 17.0103 | 13.8773 | -21.9993 | 30 | -0.4038 | 0.2331 | -4.5546 | 0.4038 | 3.547 | 0.5059 | 0.2892 | -0.2337 | 0.1476 | 0.0073 | -0.1158 | 0.4461 | -0.3977 | -0.1663 | -0.1735 | -0.1491 | -0.4908 | 0.1399 | -0.2844 | 0.1815 | -0.2697 | -6.0263 | 21.9789 | -17.2197 | 31 | 11.1999 | 2.9703 | 3.2313 | 1.4558 | -1.0264 | 0 | 0.1506 | -0.5 | 0.1933 | 0.5051 | -0.1686 | 0.1403 | -0.4551 | -0.2721 | 0.0179 | 0.8625 | -0.157 | -0.2341 | 0.3418 | 0.1728 | 0.3684 | 1.8086 | 17.5435 | -4.3534 |
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精密化 TLSグループ | ID | Refine-ID | Refine TLS-ID | Selection details | Auth asym-ID | Auth seq-ID |
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1 | X-RAY DIFFRACTION | 1 | {A|5 - 46} | A5 - 46 | 2 | X-RAY DIFFRACTION | 2 | {A|47 - 180} | A47 - 180 | 3 | X-RAY DIFFRACTION | 3 | {A|181 - 245} | A181 - 245 | 4 | X-RAY DIFFRACTION | 4 | {B|4 - 34} | B4 - 34 | 5 | X-RAY DIFFRACTION | 5 | {B|35 - 158} | B35 - 158 | 6 | X-RAY DIFFRACTION | 6 | {B|159 - 180} | B159 - 180 | 7 | X-RAY DIFFRACTION | 7 | {B|181 - 245} | B181 - 245 | 8 | X-RAY DIFFRACTION | 8 | {C|4 - 17} | C4 - 17 | 9 | X-RAY DIFFRACTION | 9 | {C|18 - 34} | C18 - 34 | 10 | X-RAY DIFFRACTION | 10 | {C|35 - 111} | C35 - 111 | 11 | X-RAY DIFFRACTION | 11 | {C|112 - 124} | C112 - 124 | 12 | X-RAY DIFFRACTION | 12 | {C|125 - 180} | C125 - 180 | 13 | X-RAY DIFFRACTION | 13 | {C|181 - 231} | C181 - 231 | 14 | X-RAY DIFFRACTION | 14 | {C|232 - 245} | C232 - 245 | 15 | X-RAY DIFFRACTION | 15 | {D|4 - 17} | D4 - 17 | 16 | X-RAY DIFFRACTION | 16 | {D|18 - 64} | D18 - 64 | 17 | X-RAY DIFFRACTION | 17 | {D|65 - 103} | D65 - 103 | 18 | X-RAY DIFFRACTION | 18 | {D|104 - 180} | D104 - 180 | 19 | X-RAY DIFFRACTION | 19 | {D|181 - 196} | D181 - 196 | 20 | X-RAY DIFFRACTION | 20 | {D|197 - 245} | D197 - 245 | 21 | X-RAY DIFFRACTION | 21 | {E|7 - 36} | E7 - 36 | 22 | X-RAY DIFFRACTION | 22 | {E|37 - 245} | E37 - 245 | 23 | X-RAY DIFFRACTION | 23 | {F|7 - 34} | F7 - 34 | 24 | X-RAY DIFFRACTION | 24 | {F|35 - 103} | F35 - 103 | 25 | X-RAY DIFFRACTION | 25 | {F|104 - 124} | F104 - 124 | 26 | X-RAY DIFFRACTION | 26 | {F|125 - 136} | F125 - 136 | 27 | X-RAY DIFFRACTION | 27 | {F|137 - 158} | F137 - 158 | 28 | X-RAY DIFFRACTION | 28 | {F|159 - 180} | F159 - 180 | 29 | X-RAY DIFFRACTION | 29 | {F|181 - 193} | F181 - 193 | 30 | X-RAY DIFFRACTION | 30 | {F|194 - 221} | F194 - 221 | 31 | X-RAY DIFFRACTION | 31 | {F|222 - 245} | F222 - 245 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
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