温度: 293 K / 手法: 蒸気拡散法, シッティングドロップ法 詳細: Protein buffer: 25 mM Tris/HCl, pH 8.0, 0.15 M NaCl, 1 mM DTT, 1 mM EDTA, 20% Glycerol Well solution: 100 mM PCPT (propionate-cacodylate-bistris propane buffer) pH 7-8, 21-24% PEG 3350, 200 ...詳細: Protein buffer: 25 mM Tris/HCl, pH 8.0, 0.15 M NaCl, 1 mM DTT, 1 mM EDTA, 20% Glycerol Well solution: 100 mM PCPT (propionate-cacodylate-bistris propane buffer) pH 7-8, 21-24% PEG 3350, 200 mM MgCl2, using 1:1 protein:reservoir solution with the protein solution at 13 mg/mL. Crystals were harvested and soaked overnight in a solution containing 100 mM PCPT, 35% PEG 3350, 200 mM Mg2Cl and 1-2 mM TK-666 from a 100 mM DMSO stock. After soaking the crystals were cryoprotected by soaking for 15 minutes in compound-soak solution supplemented with 20% ethylene glycol. , VAPOR DIFFUSION, SITTING DROP, temperature 293K PH範囲: 7-8