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- PDB-4fb1: Crystal Structure of WT MauG in Complex with Pre-Methylamine Dehy... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4fb1 | ||||||
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Title | Crystal Structure of WT MauG in Complex with Pre-Methylamine Dehydrogenase Aged 60 Days | ||||||
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![]() | Oxidoreductase/Electron Transfer / trytpophan tryptophylquinone / Oxidoreductase-Electron Transfe complex / Oxidoreductase-Electron Transfer complex | ||||||
Function / homology | ![]() methylamine dehydrogenase (amicyanin) / methylamine dehydrogenase (amicyanin) activity / methylamine metabolic process / aliphatic amine dehydrogenase activity / amine metabolic process / Oxidoreductases / cytochrome-c peroxidase activity / outer membrane-bounded periplasmic space / periplasmic space / electron transfer activity ...methylamine dehydrogenase (amicyanin) / methylamine dehydrogenase (amicyanin) activity / methylamine metabolic process / aliphatic amine dehydrogenase activity / amine metabolic process / Oxidoreductases / cytochrome-c peroxidase activity / outer membrane-bounded periplasmic space / periplasmic space / electron transfer activity / heme binding / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Yukl, E.T. / Wilmot, C.M. | ||||||
![]() | ![]() Title: Diradical intermediate within the context of tryptophan tryptophylquinone biosynthesis. Authors: Yukl, E.T. / Liu, F. / Krzystek, J. / Shin, S. / Jensen, L.M. / Davidson, V.L. / Wilmot, C.M. / Liu, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 690.7 KB | Display | ![]() |
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PDB format | ![]() | 571.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4fa1C ![]() 4fa4C ![]() 4fa5C ![]() 4fa9C ![]() 4fanC ![]() 4favC ![]() 3l4mS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Details | Pre-methylamine dehydrogenase exists as a heterotetramer of 2 heavy and 2 light chains. 2 molecules of MauG associate with the tetramer to form a 6 chain complex in the asymmetric unit. |
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Components
-Protein / Antibody / Methylamine dehydrogenase ... , 3 types, 6 molecules ABCEDF
#1: Protein | Mass: 41146.629 Da / Num. of mol.: 2 / Fragment: unp residues 21-387 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Antibody | Mass: 15025.595 Da / Num. of mol.: 2 / Fragment: unp residues 58-188 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() References: UniProt: P22619, methylamine dehydrogenase (amicyanin) #3: Protein | Mass: 42321.152 Da / Num. of mol.: 2 / Fragment: unp residues 33-417 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
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-Non-polymers , 5 types, 665 molecules ![](data/chem/img/CA.gif)
![](data/chem/img/HEC.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/MES.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/HEC.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/MES.gif)
![](data/chem/img/HOH.gif)
#4: Chemical | #5: Chemical | ChemComp-HEC / #6: Chemical | #7: Chemical | #8: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.28 Å3/Da / Density % sol: 45.94 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.4 Details: 0.1M MES pH 6.4, 0.1M sodium acetate, 22-26 % w/v PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Jul 28, 2011 / Details: BIOMORPH MIRRORS (KIRKPATRICK- BAEZ CONFIGURATION) |
Radiation | Monochromator: SI(111) DOUBLE CRYSTAL MONOCHROMATOR / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.03314 Å / Relative weight: 1 |
Reflection | Resolution: 2.15→50 Å / Num. all: 96535 / Num. obs: 93015 / % possible obs: 96.4 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 4.3 % / Rmerge(I) obs: 0.087 / Net I/σ(I): 15.58 |
Reflection shell | Resolution: 2.15→2.19 Å / Redundancy: 3.7 % / Rmerge(I) obs: 0.549 / Mean I/σ(I) obs: 2 / % possible all: 81.3 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3L4M Resolution: 2.15→44.49 Å / Cor.coef. Fo:Fc: 0.971 / Cor.coef. Fo:Fc free: 0.945 / SU B: 13.097 / SU ML: 0.152 / Cross valid method: THROUGHOUT / ESU R: 0.245 / ESU R Free: 0.198 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 45.897 Å2
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Refinement step | Cycle: LAST / Resolution: 2.15→44.49 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.15→2.206 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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