Crystal structure of mouse ryanodine receptor 2 (2699-2904)
要素
Ryanodine receptor 2
キーワード
METAL TRANSPORT / Ryanodine Receptor Calcium Release Channel (リアノジン受容体) / Phosphorylation (リン酸化) / Muscle (骨格筋) / Cardiac (心臓)
機能・相同性
機能・相同性情報
manganese ion transmembrane transport / suramin binding / establishment of protein localization to endoplasmic reticulum / type B pancreatic cell apoptotic process / Purkinje myocyte to ventricular cardiac muscle cell signaling / regulation of SA node cell action potential / regulation of atrial cardiac muscle cell action potential / left ventricular cardiac muscle tissue morphogenesis / organic cyclic compound binding / regulation of AV node cell action potential ...manganese ion transmembrane transport / suramin binding / establishment of protein localization to endoplasmic reticulum / type B pancreatic cell apoptotic process / Purkinje myocyte to ventricular cardiac muscle cell signaling / regulation of SA node cell action potential / regulation of atrial cardiac muscle cell action potential / left ventricular cardiac muscle tissue morphogenesis / organic cyclic compound binding / regulation of AV node cell action potential / calcium-induced calcium release activity / sarcoplasmic reticulum calcium ion transport / Stimuli-sensing channels / Ion homeostasis / ventricular cardiac muscle cell action potential / regulation of ventricular cardiac muscle cell action potential / positive regulation of sequestering of calcium ion / embryonic heart tube morphogenesis / cardiac muscle hypertrophy / ryanodine-sensitive calcium-release channel activity / response to muscle activity / release of sequestered calcium ion into cytosol by sarcoplasmic reticulum / calcium ion transport into cytosol / regulation of cardiac muscle contraction by calcium ion signaling / calcium ion transmembrane import into cytosol / response to caffeine / A band / response to redox state / positive regulation of heart rate / negative regulation of cytosolic calcium ion concentration / cellular response to caffeine / protein kinase A regulatory subunit binding / intracellularly gated calcium channel activity / protein kinase A catalytic subunit binding / positive regulation of the force of heart contraction / response to magnesium ion / : / detection of calcium ion / 小胞体 / regulation of cardiac muscle contraction / regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion / striated muscle contraction / release of sequestered calcium ion into cytosol / cardiac muscle contraction / extrinsic component of cytoplasmic side of plasma membrane / monoatomic ion transmembrane transport / sarcoplasmic reticulum membrane / cellular response to epinephrine stimulus / calcium channel complex / regulation of cytosolic calcium ion concentration / response to muscle stretch / regulation of heart rate / sarcomere / 筋小胞体 / establishment of localization in cell / calcium-mediated signaling / calcium ion transmembrane transport / calcium channel activity / 筋鞘 / Z disc / response to calcium ion / intracellular calcium ion homeostasis / calcium ion transport / : / 核膜 / scaffold protein binding / calmodulin binding / response to hypoxia / calcium ion binding / protein kinase binding / enzyme binding / protein-containing complex / 生体膜 / identical protein binding 類似検索 - 分子機能
モノクロメーター: Double Crystal Monochromator with cryo-cooled 1st crystal sagittally bent 2nd crystal followed by vertically focusing mirror プロトコル: MAD / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 0.9795 Å / 相対比: 1
反射
解像度: 1.65→50 Å / Num. all: 58433 / Num. obs: 58410 / % possible obs: 100 % / Observed criterion σ(I): -3 / Biso Wilson estimate: 31.434 Å2 / Rmerge(I) obs: 0.141 / Net I/σ(I): 9.69
反射 シェル
Rmerge(I) obs: 0.016 / Diffraction-ID: 1
解像度 (Å)
最高解像度 (Å)
Mean I/σ(I) obs
Num. measured obs
Num. unique obs
% possible all
1.65-1.69
1.94
30736
4241
100
1.69-1.74
2.37
30545
4192
100
1.74-1.79
2.9
29766
4043
100
1.79-1.84
3.55
29132
3953
100
1.84-1.91
4.46
30107
3773
99.9
1.91-1.97
6.91
54698
3711
100
1.97-2.05
9.26
52619
3572
100
2.05-2.13
11.26
50177
3430
100
2.13-2.22
12.87
48134
3304
100
2.22-2.33
14.45
45721
3178
100
2.33-2.46
15.84
43027
3014
100
2.46-2.61
17.24
40343
2841
100
2.61-2.79
15.34
28767
2703
100
2.79-3.01
13.07
16660
2514
100
3.01-3.3
14.22
15212
2329
99.9
3.3-3.69
14.96
13657
2129
100
3.69-4.26
15.49
11870
1866
99.9
4.26-5.22
15.5
10003
1605
99.9
5.22-7.38
14.78
7533
1278
99.8
7.38
14.92
4056
734
99.1
-
解析
ソフトウェア
名称
バージョン
分類
NB
XSCALE
データスケーリング
REFMAC
5.6.0117
精密化
PDB_EXTRACT
3.11
データ抽出
MxDC
GUI
データ収集
XDS
データ削減
PHENIX
位相決定
精密化
構造決定の手法: 多波長異常分散 / 解像度: 1.65→49.05 Å / Cor.coef. Fo:Fc: 0.938 / Cor.coef. Fo:Fc free: 0.929 / Occupancy max: 1 / Occupancy min: 0.5 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R: 0.101 / ESU R Free: 0.098 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT U VALUES : REFINED INDIVIDUALLY
Rfactor
反射数
%反射
Selection details
Rfree
0.2501
2921
5 %
RANDOM
Rwork
0.2282
-
-
-
obs
0.2293
55490
99.92 %
-
all
-
58433
-
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK