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- PDB-4ep7: Functional implications from the Cid1 poly(U) polymerase crystal ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4ep7 | ||||||
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Title | Functional implications from the Cid1 poly(U) polymerase crystal structure | ||||||
![]() | Poly(A) RNA polymerase protein cid1 | ||||||
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Function / homology | ![]() polyuridylation-dependent decapping of nuclear-transcribed mRNA / UTP binding / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Munoz-Tello, P. / Gabus, C. / Thore, S. | ||||||
![]() | ![]() Title: Functional implications from the cid1 poly(u) polymerase crystal structure. Authors: Munoz-Tello, P. / Gabus, C. / Thore, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 141.7 KB | Display | ![]() |
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PDB format | ![]() | 115.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 863.4 KB | Display | ![]() |
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Full document | ![]() | 877.8 KB | Display | |
Data in XML | ![]() | 29.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 38971.660 Da / Num. of mol.: 2 / Fragment: UNP Residues 40-377 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Strain: 972 / ATCC 24843 / Gene: cid1, SPAC19D5.03 / Production host: ![]() ![]() ![]() References: UniProt: O13833, ![]() #2: Chemical | ![]() #3: Chemical | ChemComp-MG / #4: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.16 Å3/Da / Density % sol: 43.01 % |
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Crystal grow![]() | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 6.1 Details: 0.1M Imidatole/MES pH 6.1, 20% Glycerol, 10% PEG 4000, 42mM NaI, 42mM NaBr, 42mM NaF., VAPOR DIFFUSION, SITTING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||||||||
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jul 5, 2011 | ||||||||||||||||||||||||||||||
Radiation | Monochromator: Channel cut ESRF monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength![]() | ||||||||||||||||||||||||||||||
Reflection | Resolution: 2.27→45.193 Å / Num. obs: 29999 / % possible obs: 97.4 % / Observed criterion σ(F): 2.64 / Observed criterion σ(I): 2.64 / Redundancy: 2.79 % / Rmerge(I) obs: 0.13 / Net I/σ(I): 11.5 | ||||||||||||||||||||||||||||||
Reflection shell |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 42.058 Å2 / ksol: 0.362 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.2805→45.193 Å
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Refine LS restraints |
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LS refinement shell |
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