- PDB-4ejm: Crystal structure of a putative zinc-binding dehydrogenase (Targe... -
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基本情報
登録情報
データベース: PDB / ID: 4ejm
タイトル
Crystal structure of a putative zinc-binding dehydrogenase (Target PSI-012003) from Sinorhizobium meliloti 1021 bound to NADP
要素
Putative zinc-binding dehydrogenase
キーワード
OXIDOREDUCTASE / NADP / Structural genomics / NYSGRC / PSI-Biology / New York Structural Genomics Research Consortium
機能・相同性
機能・相同性情報
酸化還元酵素; CH-OHの結合に対し酸化酵素として働く; NAD又はNADPを用いる / oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor / nucleotide binding / zinc ion binding 類似検索 - 分子機能
温度: 298 K / pH: 6.2 詳細: Protein (20 mM Hepes, pH 7.5, 150 mM NaCl, 10% glycerol; Reservoir (MCSG2 #1: 0.2 M Potassium Sodium Tartarate and 20% (w/v) PEG3350 ); Cryoprotection (30% Ethylene glycol); Crystals soaked ...詳細: Protein (20 mM Hepes, pH 7.5, 150 mM NaCl, 10% glycerol; Reservoir (MCSG2 #1: 0.2 M Potassium Sodium Tartarate and 20% (w/v) PEG3350 ); Cryoprotection (30% Ethylene glycol); Crystals soaked in 100mM NADP and 100mM 3-hydroxybutyrate-Na in 15% PEG3350 and 30% ethyleneglycol for at least 30mins, temperature 298K, VAPOR DIFFUSION, SITTING DROP