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Open data
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Basic information
Entry | Database: PDB / ID: 1kev | ||||||
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Title | STRUCTURE OF NADP-DEPENDENT ALCOHOL DEHYDROGENASE | ||||||
![]() | NADP-DEPENDENT ALCOHOL DEHYDROGENASE | ||||||
![]() | OXIDOREDUCTASE / ZINC / NADP | ||||||
Function / homology | ![]() isopropanol dehydrogenase (NADP+) / isopropanol dehydrogenase (NADP+) activity / zinc ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Korkhin, Y. / Frolow, F. | ||||||
![]() | ![]() Title: Crystalline alcohol dehydrogenases from the mesophilic bacterium Clostridium beijerinckii and the thermophilic bacterium Thermoanaerobium brockii: preparation, characterization and molecular symmetry. Authors: Korkhin, Y. / Frolow, F. / Bogin, O. / Peretz, M. / Kalb, A.J. / Burstein, Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 284.8 KB | Display | ![]() |
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PDB format | ![]() | 232 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 681.8 KB | Display | ![]() |
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Full document | ![]() | 702.4 KB | Display | |
Data in XML | ![]() | 31.3 KB | Display | |
Data in CIF | ![]() | 48.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1pedSC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Protein | Mass: 37762.891 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Details: HOLO-ENZYME FORM / Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-ZN / #3: Chemical | ChemComp-NDP / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.94 Å3/Da / Density % sol: 32 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 8.2 / Details: SEE REFERENCE 1, pH 8.2 | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, sitting drop | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Sep 22, 1995 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.2748 Å / Relative weight: 1 |
Reflection | Resolution: 2.05→50 Å / Num. obs: 97572 / % possible obs: 88.2 % / Observed criterion σ(I): 0 / Redundancy: 3.33 % / Biso Wilson estimate: 27.5 Å2 / Rsym value: 0.058 / Net I/σ(I): 18.1 |
Reflection shell | Resolution: 2.05→2.09 Å / Redundancy: 2.97 % / Mean I/σ(I) obs: 3.19 / Rsym value: 0.232 / % possible all: 69.3 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1PED Resolution: 2.05→50 Å / Cross valid method: SHELLS OF RESOLUTION / σ(F): 0
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Displacement parameters | Biso mean: 31 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.28 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.05→50 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.05→2.1 Å / Total num. of bins used: 15
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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