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- PDB-4ec2: Crystal structure of trimeric frataxin from the yeast Saccharomyc... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4ec2 | ||||||
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Title | Crystal structure of trimeric frataxin from the yeast Saccharomyces cerevisiae, complexed with ferrous | ||||||
![]() | Frataxin homolog, mitochondrial | ||||||
![]() | TRANSPORT PROTEIN / alpha/beta sandwich / metallochaperone / iron-storage | ||||||
Function / homology | ![]() Mitochondrial iron-sulfur cluster biogenesis / Mitochondrial protein import / mitochondrial electron transport, succinate to ubiquinone / iron chaperone activity / iron-sulfur cluster assembly complex / response to iron(II) ion / iron-sulfur cluster assembly / heme biosynthetic process / ferroxidase / ferroxidase activity ...Mitochondrial iron-sulfur cluster biogenesis / Mitochondrial protein import / mitochondrial electron transport, succinate to ubiquinone / iron chaperone activity / iron-sulfur cluster assembly complex / response to iron(II) ion / iron-sulfur cluster assembly / heme biosynthetic process / ferroxidase / ferroxidase activity / glutathione metabolic process / ferric iron binding / ferrous iron binding / mitochondrial intermembrane space / 2 iron, 2 sulfur cluster binding / iron ion transport / intracellular iron ion homeostasis / response to oxidative stress / mitochondrial inner membrane / mitochondrial matrix / mitochondrion / identical protein binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Soderberg, C.A.G. / Rajan, S. / Gakh, O. / Isaya, G. / Al-Karadaghi, S. | ||||||
![]() | ![]() Title: The molecular basis of iron-induced oligomerization of frataxin and the role of the ferroxidation reaction in oligomerization. Authors: Soderberg, C.A. / Rajan, S. / Shkumatov, A.V. / Gakh, O. / Schaefer, S. / Ahlgren, E.C. / Svergun, D.I. / Isaya, G. / Al-Karadaghi, S. #1: ![]() Title: Oligomerization propensity and flexibility of yeast frataxin studied by x-ray crystallography and small-angle x-ray scattering Authors: Soderberg, C.A.G. / Shkumatov, A.V. / Rajan, S. / Gakh, O. / Svergun, D.I. / Isaya, G. / Al-karadaghi, S. #2: ![]() Title: The structures of frataxin oligomers reveal the mechanism for the delivery and detoxification of iron Authors: Karlberg, T. / Schagerlof, U. / Gakh, O. / Park, S. / Ryde, U. / Lindahl, M. / Leath, K. / Garman, E. / Isaya, G. / Al-karadaghi, S. | ||||||
History |
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Remark 650 | HELIX DETERMINATION METHOD: AUTHOR | ||||||
Remark 700 | SHEET DETERMINATION METHOD: AUTHOR |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 36.1 KB | Display | ![]() |
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PDB format | ![]() | 24.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 429.7 KB | Display | ![]() |
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Full document | ![]() | 431.8 KB | Display | |
Data in XML | ![]() | 6.6 KB | Display | |
Data in CIF | ![]() | 7.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2fqlS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 13671.182 Da / Num. of mol.: 1 / Fragment: UNP residues 52-174 / Mutation: Y73A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: ATCC 204508 / S288c / Gene: YFH1, YDL120W / Production host: ![]() ![]() |
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#2: Chemical | ChemComp-FE2 / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 5.48 Å3/Da / Density % sol: 77.54 % |
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Crystal grow | Temperature: 288 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: 1.7 M (NH4)2SO4, 0.2 M Li2SO4, 4 % Gamma-butyrolactone, 0.1 M Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Sep 10, 2008 |
Radiation | Monochromator: SI (111) DOUBLE CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.038 Å / Relative weight: 1 |
Reflection | Resolution: 3→50 Å / Num. obs: 11254 / % possible obs: 96.9 % / Redundancy: 3.32 % / Rmerge(I) obs: 0.064 / Net I/σ(I): 11.62 |
Reflection shell | Resolution: 3→3.08 Å / Redundancy: 3.12 % / Rmerge(I) obs: 0.632 / Mean I/σ(I) obs: 1.76 / Num. unique all: 791 / % possible all: 93.3 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2FQL Resolution: 3.002→24.815 Å / SU ML: 1.16 / σ(F): 1.29 / Phase error: 39.05 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.86 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 59.031 Å2 / ksol: 0.287 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.002→24.815 Å
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Refine LS restraints |
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LS refinement shell |
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