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Yorodumi- PDB-4e15: Crystal structure of kynurenine formamidase conjugated with an in... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4.0E+15 | ||||||
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Title | Crystal structure of kynurenine formamidase conjugated with an inhibitor | ||||||
Components | kynurenine formamidase | ||||||
Keywords | HYDROLASE/HYDROLASE INHIBITOR / alpha/beta hydrolase fold / HYDROLASE-HYDROLASE INHIBITOR complex | ||||||
Function / homology | Function and homology information arylformamidase / arylformamidase activity / lipase activity / tryptophan catabolic process to kynurenine Similarity search - Function | ||||||
Biological species | Drosophila melanogaster (fruit fly) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.5 Å | ||||||
Authors | Han, Q. / Robinson, H. / Li, J. | ||||||
Citation | Journal: Biochem.J. / Year: 2012 Title: Biochemical identification and crystal structure of kynurenine formamidase from Drosophila melanogaster. Authors: Han, Q. / Robinson, H. / Li, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4e15.cif.gz | 144.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4e15.ent.gz | 113.2 KB | Display | PDB format |
PDBx/mmJSON format | 4e15.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 4e15_validation.pdf.gz | 458.7 KB | Display | wwPDB validaton report |
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Full document | 4e15_full_validation.pdf.gz | 469.2 KB | Display | |
Data in XML | 4e15_validation.xml.gz | 30.4 KB | Display | |
Data in CIF | 4e15_validation.cif.gz | 44.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/e1/4e15 ftp://data.pdbj.org/pub/pdb/validation_reports/e1/4e15 | HTTPS FTP |
-Related structure data
Related structure data | 4e11SC 4e14C S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 35372.098 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Drosophila melanogaster (fruit fly) / Gene: CG9542, Dmel_CG9542 / Plasmid: PTYB / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q9VMC9, arylformamidase #2: Chemical | ChemComp-EDO / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.02 Å3/Da / Density % sol: 39.21 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 0.1 M HEPES, pH 7.5, 18% PEG4000, 8% 2-propanol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X29A / Wavelength: 1.075 Å |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Nov 29, 2010 |
Radiation | Monochromator: double crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.075 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→50 Å / Num. all: 91964 / Num. obs: 90494 / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 14.3 % / Rmerge(I) obs: 0.15 / Net I/σ(I): 25 |
Reflection shell | Resolution: 1.5→1.55 Å / Redundancy: 12.5 % / Rmerge(I) obs: 0.34 / Mean I/σ(I) obs: 4 / Num. unique all: 7888 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 4E11 Resolution: 1.5→46.7 Å / Cor.coef. Fo:Fc: 0.961 / Cor.coef. Fo:Fc free: 0.954 / Cross valid method: THROUGHOUT / ESU R: 0.078 / ESU R Free: 0.077 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 15.872 Å2
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Refinement step | Cycle: LAST / Resolution: 1.5→46.7 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.5→1.539 Å / Total num. of bins used: 20
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