Toll Like Receptor 4 (TLR4) Cascade / Signal regulatory protein family interactions / Regulation of TLR by endogenous ligand / Surfactant metabolism / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / extracellular matrix structural constituent conferring tensile strength / respiratory gaseous exchange by respiratory system / collagen trimer / surfactant homeostasis / lung alveolus development ...Toll Like Receptor 4 (TLR4) Cascade / Signal regulatory protein family interactions / Regulation of TLR by endogenous ligand / Surfactant metabolism / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / extracellular matrix structural constituent conferring tensile strength / respiratory gaseous exchange by respiratory system / collagen trimer / surfactant homeostasis / lung alveolus development / positive regulation of phagocytosis / multivesicular body / extracellular matrix organization / carbohydrate binding / collagen-containing extracellular matrix / innate immune response / extracellular space Similarity search - Function
Lung surfactant protein D coiled-coil trimerisation / Lung surfactant protein D coiled-coil trimerisation / : / Collagen triple helix repeat / Collagen triple helix repeat (20 copies) / C-type lectin, conserved site / C-type lectin domain signature. / Mannose-Binding Protein A; Chain A / Mannose-Binding Protein A, subunit A / Lectin C-type domain ...Lung surfactant protein D coiled-coil trimerisation / Lung surfactant protein D coiled-coil trimerisation / : / Collagen triple helix repeat / Collagen triple helix repeat (20 copies) / C-type lectin, conserved site / C-type lectin domain signature. / Mannose-Binding Protein A; Chain A / Mannose-Binding Protein A, subunit A / Lectin C-type domain / C-type lectin domain profile. / C-type lectin-like / C-type lectin (CTL) or carbohydrate-recognition domain (CRD) / C-type lectin-like/link domain superfamily / C-type lectin fold / Roll / Alpha Beta Similarity search - Domain/homology
Mass: 18.015 Da / Num. of mol.: 36 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.44 Å3/Da / Density % sol: 49.49 %
Crystal grow
Temperature: 290 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 12.3 mg/ml protein in 0.9 % sodium chloride, 5 mM EDTA, 15 mM calcium chloride, and 20 mM mannose was mixed with an equal volume of 0.1 M bistris (pH =6.5), 20% w/v PEG 3350, 200 mM magesium ...Details: 12.3 mg/ml protein in 0.9 % sodium chloride, 5 mM EDTA, 15 mM calcium chloride, and 20 mM mannose was mixed with an equal volume of 0.1 M bistris (pH =6.5), 20% w/v PEG 3350, 200 mM magesium chloride, and 15% v/v glycerol. Prior to data collection crystals were soaked in a 30% w/v D-mannose solution., VAPOR DIFFUSION, HANGING DROP, temperature 290K
Resolution: 2.2→2.28 Å / Redundancy: 9.4 % / Rmerge(I) obs: 0.418 / Mean I/σ(I) obs: 7.25 / Num. unique all: 839 / % possible all: 99.3
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Processing
Software
Name
Version
Classification
CrystalClear
datacollection
PHASER
phasing
PHENIX
(phenix.refine: 1.6.3_473)
refinement
DENZO
datareduction
SCALEPACK
datascaling
Refinement
Method to determine structure: FOURIER SYNTHESIS Starting model: A complex of pig SPD and glycerol solved by molecular replacement in the same space group using human SPD (pdb code 2GGU B chain)
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