Crystal Structure of V151L Actinorhodin Polyketide Ketoreductase with NADPH
Components
Ketoacyl reductase
Keywords
OXIDOREDUCTASE / Rossmann fold / ketoreductase
Function / homology
Function and homology information
Oxidoreductases; Acting on the CH-CH group of donors; With NAD+ or NADP+ as acceptor / steroid metabolic process / antibiotic biosynthetic process / oxidoreductase activity Similarity search - Function
Mass: 29478.389 Da / Num. of mol.: 2 / Mutation: V151L Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptomyces coelicolor (bacteria) / Gene: actIII, SCBAC28G1.12c, SCO5086 / Plasmid: pET28 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) References: UniProt: P16544, Oxidoreductases; Acting on the CH-CH group of donors; With NAD+ or NADP+ as acceptor
Mass: 18.015 Da / Num. of mol.: 44 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 3.16 Å3/Da / Density % sol: 61.08 %
Crystal grow
Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 9 Details: 0.1 M Tris-Cl, 1.8 M Na malonate, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
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Data collection
Diffraction
Mean temperature: 100 K
Diffraction source
Source: SYNCHROTRON / Site: ALS / Beamline: 8.2.2 / Wavelength: 0.9998 Å
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