Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.26 Å3/Da / Density % sol: 45.6 % / Description: NONE
Crystal grow
Temperature: 293 K Details: PROTEIN (1 UL AT 25.0 MG/ML) WAS MIXED WITH 1 UL RESERVOIR SOLUTION AND EQUILIBRATED AT 20 C AGAINST 500 UL RESERVOIRS CONTAINING 0.1 M HEPES PH 7.5, 1.6 M AMMONIUM SULFATE AND 1.5% (VOL/VOL) ...Details: PROTEIN (1 UL AT 25.0 MG/ML) WAS MIXED WITH 1 UL RESERVOIR SOLUTION AND EQUILIBRATED AT 20 C AGAINST 500 UL RESERVOIRS CONTAINING 0.1 M HEPES PH 7.5, 1.6 M AMMONIUM SULFATE AND 1.5% (VOL/VOL) PEG 400. CRYSTALS WERE CRYOPROTECTED BY PASSAGE THROUGH 2 UL OF PERFLUOROPOLYETHER OIL (HAMPTON RESEARCH) THAT HAD BEEN OVERLAID ONTO THE MOTHER LIQUOR.
Resolution: 1.84→45.71 Å / Cor.coef. Fo:Fc: 0.964 / Cor.coef. Fo:Fc free: 0.952 / SU B: 8.18 / SU ML: 0.122 / Cross valid method: THROUGHOUT / ESU R: 0.163 / ESU R Free: 0.145 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES WITH TLS ADDED.
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.23888
5665
5 %
RANDOM
Rwork
0.20638
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-
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obs
0.20801
107126
97.61 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK