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- PDB-4csy: E-selectin lectin, EGF-like and two SCR domains complexed with Si... -
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Basic information
Entry | Database: PDB / ID: 4csy | |||||||||
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Title | E-selectin lectin, EGF-like and two SCR domains complexed with Sialyl Lewis X | |||||||||
![]() | E-SELECTIN | |||||||||
![]() | CELL ADHESION / CELL-ADHESION / HUMAN LECTIN / C-TYPE LECTIN / INFLAMMATION / LEUKOCYTE / SIALYL LEWIS X / SLEX / PROTEIN CONFORMATION / LIGAND-INDUCED CONFORMATIONAL CHANGE / CATCH- BOND | |||||||||
Function / homology | ![]() actin filament-based process / positive regulation of leukocyte tethering or rolling / sialic acid binding / oligosaccharide binding / leukocyte migration involved in inflammatory response / leukocyte tethering or rolling / positive regulation of leukocyte migration / heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules / leukocyte cell-cell adhesion / cortical cytoskeleton ...actin filament-based process / positive regulation of leukocyte tethering or rolling / sialic acid binding / oligosaccharide binding / leukocyte migration involved in inflammatory response / leukocyte tethering or rolling / positive regulation of leukocyte migration / heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules / leukocyte cell-cell adhesion / cortical cytoskeleton / phospholipase binding / positive regulation of receptor internalization / : / response to tumor necrosis factor / clathrin-coated pit / response to interleukin-1 / response to cytokine / caveola / Cell surface interactions at the vascular wall / calcium-mediated signaling / transmembrane signaling receptor activity / regulation of inflammatory response / response to lipopolysaccharide / inflammatory response / membrane raft / external side of plasma membrane / perinuclear region of cytoplasm / extracellular space / metal ion binding / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Preston, R.C. / Jakob, R.P. / Binder, F.P.C. / Sager, C.P. / Ernst, B. / Maier, T. | |||||||||
![]() | ![]() Title: E-Selectin Ligand Complexes Adopt an Extended High-Affinity Conformation. Authors: Preston, R.C. / Jakob, R.P. / Binder, F.P. / Sager, C.P. / Ernst, B. / Maier, T. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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PDBx/mmCIF format | ![]() | 254.7 KB | Display | ![]() |
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PDB format | ![]() | 207.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4c16C ![]() 1g1sS ![]() 1h04S ![]() 3govS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-1, -0.005513, -0.005256), Vector: |
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Components
#1: Protein | Mass: 31305.760 Da / Num. of mol.: 2 Fragment: LECTIN DOMAIN, EGF-LIKE DOMAIN, SHORT CONSENSUS REPEAT DOMAIN 1, SHORT CONSENSUS REPEAT DOMAIN 2, RESIDUES 22-301 Source method: isolated from a genetically manipulated source Details: N-ACETYLGLUCOSAMINE RESIDUES ATTACHED TO ASN4, ASN124, ASN139, ASN158, ASN178, ASN182, AND ASN244 ON BOTH CHAINS. Source: (gene. exp.) ![]() Cell line (production host): CHINESE HAMSTER OVARY (CHO) CELLS Production host: ![]() ![]() #2: Polysaccharide | Source method: isolated from a genetically manipulated source #3: Sugar | ChemComp-NAG / #4: Chemical | #5: Water | ChemComp-HOH / | Nonpolymer details | SIALYL LEWIS X METHYL GLUCOSIDE (DRG): SIALYL-LEWIS-X TETRASACCHARIDE, WITH A TRIMETHYLSILYLETHYL ...SIALYL LEWIS X METHYL GLUCOSIDE (DRG): SIALYL-LEWIS-X TETRASACCH | Sequence details | WITHOUT N-TERMINAL SECRETION SIGNAL (AA. 1-21). SEQUENCE OF MATURE PROTEIN STARTS WITH RESIDUE 1 ...WITHOUT N-TERMINAL SECRETION SIGNAL (AA. 1-21). SEQUENCE OF MATURE PROTEIN STARTS WITH RESIDUE 1 FOR COMPATIBIL | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.8 Å3/Da / Density % sol: 56.1 % / Description: NONE |
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Crystal grow | Details: 13 % PEG8000, HEPES, MOPS PH 6.2, CACL2, 10MM SLEX-OTMSE |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Mar 30, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.99985 Å / Relative weight: 1 |
Reflection | Resolution: 2.41→28.28 Å / Num. obs: 25239 / % possible obs: 95.4 % / Observed criterion σ(I): 2 / Redundancy: 1.8 % / Biso Wilson estimate: 66.77 Å2 / Rmerge(I) obs: 0.04 / Net I/σ(I): 8.8 |
Reflection shell | Resolution: 2.41→2.53 Å / Redundancy: 1.8 % / Rmerge(I) obs: 0.32 / Mean I/σ(I) obs: 2 / % possible all: 90.2 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRIES 1G1S, 3GOV, 1H04 Resolution: 2.41→28.32 Å / Cor.coef. Fo:Fc: 0.9294 / Cor.coef. Fo:Fc free: 0.9003 / SU R Cruickshank DPI: 0.471 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.442 / SU Rfree Blow DPI: 0.261 / SU Rfree Cruickshank DPI: 0.268 Details: DISORDER IN LOOPS 227-231, 254-259 AND N-TERMINAL RESIDUES 278-280 BUILT ACCORDING TO ACCOMPANYING DEPOSITION EBI-57874.
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Displacement parameters | Biso mean: 79.3 Å2
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Refine analyze | Luzzati coordinate error obs: 0.507 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.41→28.32 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.41→2.51 Å / Total num. of bins used: 13
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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