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- PDB-4cdk: Structure of ZNRF3-RSPO1 -

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ID or keywords:

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Basic information

Entry
Database: PDB / ID: 4cdk
TitleStructure of ZNRF3-RSPO1
Components
  • E3 UBIQUITIN-PROTEIN LIGASE ZNRF3
  • R-SPONDIN-1
KeywordsLIGASE / WNT SIGNALING / ADULT STEM CELLS / E3 LIGASE / PROTEASE-ASSOCIATED DOMAIN / ZINC RING FINGER / LGR5 / R-SPONDIN
Function / homology
Function and homology information


regulation of Wnt signaling pathway, planar cell polarity pathway / Wnt receptor catabolic process / negative regulation of non-canonical Wnt signaling pathway / Regulation of FZD by ubiquitination / regulation of receptor internalization / frizzled binding / regulation of canonical Wnt signaling pathway / limb development / negative regulation of Wnt signaling pathway / positive regulation of Wnt signaling pathway ...regulation of Wnt signaling pathway, planar cell polarity pathway / Wnt receptor catabolic process / negative regulation of non-canonical Wnt signaling pathway / Regulation of FZD by ubiquitination / regulation of receptor internalization / frizzled binding / regulation of canonical Wnt signaling pathway / limb development / negative regulation of Wnt signaling pathway / positive regulation of Wnt signaling pathway / Regulation of FZD by ubiquitination / stem cell proliferation / G protein-coupled receptor binding / negative regulation of canonical Wnt signaling pathway / RING-type E3 ubiquitin transferase / Wnt signaling pathway / ubiquitin-protein transferase activity / positive regulation of canonical Wnt signaling pathway / ubiquitin protein ligase activity / heparin binding / ubiquitin-dependent protein catabolic process / protein ubiquitination / positive regulation of protein phosphorylation / signaling receptor binding / extracellular region / metal ion binding / nucleus / plasma membrane
Similarity search - Function
E3 ubiquitin-protein ligase ZNRF3, Zinc finger, RING-type / ZNRF-3, ectodomain / ZNRF-3 Ectodomain / R-spondin, Fu-CRD domain / Furin-like repeat, cysteine-rich / Hormone Receptor, Insulin-like Growth Factor Receptor 1; Chain A domain 2 / Hormone Receptor, Insulin-like Growth Factor Receptor 1; Chain A, domain 2 / Ring finger domain / Glucose Oxidase; domain 1 - #30 / Glucose Oxidase; domain 1 ...E3 ubiquitin-protein ligase ZNRF3, Zinc finger, RING-type / ZNRF-3, ectodomain / ZNRF-3 Ectodomain / R-spondin, Fu-CRD domain / Furin-like repeat, cysteine-rich / Hormone Receptor, Insulin-like Growth Factor Receptor 1; Chain A domain 2 / Hormone Receptor, Insulin-like Growth Factor Receptor 1; Chain A, domain 2 / Ring finger domain / Glucose Oxidase; domain 1 - #30 / Glucose Oxidase; domain 1 / Thrombospondin type-1 (TSP1) repeat superfamily / Thrombospondin type-1 (TSP1) repeat profile. / Thrombospondin type 1 repeats / Thrombospondin type-1 (TSP1) repeat / Furin-like repeat / Furin-like repeats / Ring finger / Growth factor receptor cysteine-rich domain superfamily / Zinc finger RING-type profile. / Zinc finger, RING-type / 3-Layer(bba) Sandwich / Ribbon / Zinc finger, RING/FYVE/PHD-type / Mainly Beta / Alpha Beta
Similarity search - Domain/homology
R-spondin-1 / E3 ubiquitin-protein ligase ZNRF3
Similarity search - Component
Biological speciesMUS MUSCULUS (house mouse)
HOMO SAPIENS (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.8 Å
AuthorsPeng, W.C. / de Lau, W. / Madoori, P.K. / Forneris, F. / Granneman, J.C.M. / Clevers, H. / Gros, P.
CitationJournal: Plos One / Year: 2013
Title: Structures of Wnt-Antagonist Znrf3 and its Complex with R-Spondin 1 and Implications for Signaling.
Authors: Peng, W.C. / De Lau, W. / Madoori, P.K. / Forneris, F. / Granneman, J.C.M. / Clevers, H. / Gros, P.
History
DepositionNov 1, 2013Deposition site: PDBE / Processing site: PDBE
Revision 1.0Jan 8, 2014Provider: repository / Type: Initial release
Revision 1.1Apr 3, 2019Group: Data collection / Other / Source and taxonomy
Category: entity_src_gen / pdbx_database_proc / pdbx_database_status
Item: _entity_src_gen.pdbx_host_org_cell_line / _pdbx_database_status.recvd_author_approval
Remark 700 SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS BELOW ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 7-STRANDED BARREL THIS IS REPRESENTED BY A 8-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEETS PRESENTED AS "BA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 7-STRANDED BARREL THIS IS REPRESENTED BY A 8-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEETS PRESENTED AS "DA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 7-STRANDED BARREL THIS IS REPRESENTED BY A 8-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL.

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: E3 UBIQUITIN-PROTEIN LIGASE ZNRF3
B: E3 UBIQUITIN-PROTEIN LIGASE ZNRF3
C: E3 UBIQUITIN-PROTEIN LIGASE ZNRF3
D: E3 UBIQUITIN-PROTEIN LIGASE ZNRF3
E: R-SPONDIN-1
F: R-SPONDIN-1
G: R-SPONDIN-1
H: R-SPONDIN-1


Theoretical massNumber of molelcules
Total (without water)126,7728
Polymers126,7728
Non-polymers00
Water99155
1
A: E3 UBIQUITIN-PROTEIN LIGASE ZNRF3
E: R-SPONDIN-1


Theoretical massNumber of molelcules
Total (without water)31,6932
Polymers31,6932
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2270 Å2
ΔGint-10.9 kcal/mol
Surface area14230 Å2
MethodPISA
2
C: E3 UBIQUITIN-PROTEIN LIGASE ZNRF3
G: R-SPONDIN-1


Theoretical massNumber of molelcules
Total (without water)31,6932
Polymers31,6932
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2370 Å2
ΔGint-9.2 kcal/mol
Surface area14430 Å2
MethodPISA
3
D: E3 UBIQUITIN-PROTEIN LIGASE ZNRF3
H: R-SPONDIN-1


Theoretical massNumber of molelcules
Total (without water)31,6932
Polymers31,6932
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1870 Å2
ΔGint-7.8 kcal/mol
Surface area14170 Å2
MethodPISA
4
B: E3 UBIQUITIN-PROTEIN LIGASE ZNRF3
F: R-SPONDIN-1


Theoretical massNumber of molelcules
Total (without water)31,6932
Polymers31,6932
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1920 Å2
ΔGint-7.5 kcal/mol
Surface area14210 Å2
MethodPISA
Unit cell
Length a, b, c (Å)51.700, 80.160, 82.980
Angle α, β, γ (deg.)66.27, 81.36, 80.66
Int Tables number1
Space group name H-MP1
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-ID
11
22
/ NCS ensembles :
ID
1
2

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Components

#1: Protein
E3 UBIQUITIN-PROTEIN LIGASE ZNRF3 / ZINC/RING FINGER PROTEIN 3


Mass: 17986.316 Da / Num. of mol.: 4 / Fragment: ECTODOMAIN, RESIDUES 53-205
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) MUS MUSCULUS (house mouse) / Cell line (production host): HEK293 / Production host: HOMO SAPIENS (human) / References: UniProt: Q5SSZ7
#2: Protein
R-SPONDIN-1 / ROOF PLATE-SPECIFIC SPONDIN-1 / HRSPO1 / R-SPONDIN 1


Mass: 13706.780 Da / Num. of mol.: 4 / Fragment: FURIN-LIKE DOMAIN, RESIDUES 31-145
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human) / Cell line (production host): HEK293 / Production host: HOMO SAPIENS (human) / References: UniProt: Q2MKA7
#3: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 55 / Source method: isolated from a natural source / Formula: H2O
Sequence detailsGS AT N-TERMINUS AND AAAHHHHHH AT C-TERMINUS ARE CLONING ARTIFACTS.

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION

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Sample preparation

CrystalDensity Matthews: 2.73 Å3/Da / Density % sol: 55 % / Description: NONE
Crystal growDetails: 0.2M SODIUM BROMIDE AND 20% W/V PEG 3350

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: SLS / Beamline: X06SA / Wavelength: 1
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.8→75.63 Å / Num. obs: 29157 / % possible obs: 95.4 % / Observed criterion σ(I): 3.2 / Redundancy: 2.2 % / Biso Wilson estimate: 54.27 Å2 / Rmerge(I) obs: 0.09
Reflection shellResolution: 2.8→75.63 Å

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Processing

SoftwareName: PHENIX / Version: (PHENIX.REFINE) / Classification: refinement
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.8→44.706 Å / SU ML: 0.34 / σ(F): 1.97 / Phase error: 27.5 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2464 1437 5.1 %
Rwork0.2177 --
obs0.2192 28133 95.3 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 83.8 Å2
Refinement stepCycle: LAST / Resolution: 2.8→44.706 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms7896 0 0 55 7951
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0058111
X-RAY DIFFRACTIONf_angle_d1.20910912
X-RAY DIFFRACTIONf_dihedral_angle_d11.9673058
X-RAY DIFFRACTIONf_chiral_restr0.0481207
X-RAY DIFFRACTIONf_plane_restr0.0061442
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.8001-2.90020.32891500.28742668X-RAY DIFFRACTION95
2.9002-3.01620.26061380.26112665X-RAY DIFFRACTION95
3.0162-3.15350.29041230.26282694X-RAY DIFFRACTION95
3.1535-3.31970.27521450.24322680X-RAY DIFFRACTION96
3.3197-3.52760.26171280.23642659X-RAY DIFFRACTION94
3.5276-3.79980.27031590.23572647X-RAY DIFFRACTION95
3.7998-4.1820.26461510.20582676X-RAY DIFFRACTION97
4.182-4.78650.16891350.17542677X-RAY DIFFRACTION95
4.7865-6.02820.23731580.20992660X-RAY DIFFRACTION95
6.0282-44.71210.24391500.20262670X-RAY DIFFRACTION96
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.57570.0601-0.47080.24990.01510.24370.18460.10720.31730.0792-0.4661-0.3397-0.15440.7159-0.01240.3310.0351-0.02410.41280.01780.34327.972619.437219.4723
20.2604-0.0124-0.0110.1353-0.1630.2882-0.1630.14440.2848-0.2217-0.18240.0669-0.10380.1487-0.04780.29090.0276-0.07720.2611-0.06850.21512.440614.4614.8379
30.01570.02110.00490.01860.0070.0072-0.1630.2111-0.38430.00080.17680.18270.0222-0.1081-0.00110.3993-0.0333-0.07580.3802-0.06910.4502-8.08841.40213.6863
40.8640.36370.15370.9996-0.13020.0085-0.1766-0.0941-0.3384-0.09930.179-0.0556-0.1473-0.5015-0.07160.3151-0.0005-0.08090.3507-0.00750.3085-1.63743.494718.8322
50.22330.1715-0.29270.5541-0.22150.68620.477-1.5829-1.5663-0.0143-0.3264-0.75520.1707-0.1124-1.02920.25030.17530.13760.4696-0.3152-0.80682.98357.05326.7637
60.2436-0.1162-0.22550.1906-0.02110.47950.452-0.20590.22720.08050.1871-0.144-0.1870.15770.29420.381-0.098-0.00580.3306-0.15590.41657.212618.41218.1923
70.34430.2320.2040.25630.05280.5024-0.1098-0.2145-0.1083-0.53270.00390.09930.65660.2596-0.10330.4473-0.0067-0.13760.3139-0.08550.350820.507911.19158.9282
80.0503-0.0442-0.12420.0555-0.09780.5006-0.1179-0.1881-0.2342-0.4529-0.21890.4850.24360.0289-0.07990.4264-0.0452-0.09910.3511-0.14220.708111.91873.67684.0195
90.10090.1125-0.20470.1493-0.26640.4475-0.3944-0.26950.2444-0.0469-0.139-0.43340.0357-0.0482-0.1180.2762-0.0665-0.0350.4026-0.08150.537524.646922.977715.6177
100.0139-0.0224-0.02720.08240.09990.09360.2751-0.05190.0298-0.23150.015-0.0984-0.3832-0.08810.28060.5251-0.1831-0.07330.385-0.22240.033515.701323.33850.265
110.0181-0.0042-0.00340.05-0.0970.02910.04750.2721-0.2710.1851-0.1660.1004-0.21670.0231-0.0010.43920.01120.00620.37050.04930.309811.72427.53281.0183
120.027-0.001-0.0131-0.00640.03080.02850.111-0.12230.0376-0.2462-0.3443-0.0530.18080.2005-0.00010.67320.08840.08810.54610.06340.540128.458633.5164-4.3409
130.1577-0.2176-0.10860.32140.06880.04140.2447-0.01960.1701-0.0825-0.31220.2992-0.4036-0.4275-0.25370.4250.02230.00850.2552-0.10650.403818.011128.71545.949
140.41790.16220.4040.07470.14880.32350.4042-0.2310.56380.0533-0.2226-0.6871-0.17540.01170.01010.2351-0.0480.05350.2394-0.03840.646727.596827.74089.2327
150.01660.0286-0.00390.06450.02630.03450.0407-0.1009-0.0451-0.6912-0.4144-0.17390.42440.2491-0.00140.45130.06940.07930.5236-0.06280.315423.881913.1734-2.9321
160.3009-0.1389-0.11820.14030.02050.051-0.5704-0.4058-0.10480.3242-0.0418-0.0540.29040.4142-0.13960.4698-0.1893-0.23630.061-0.748-0.302117.666513.27139.966
170.2692-0.075-0.13950.104-0.24210.23270.2041-0.1268-0.5124-0.0360.04590.1301-0.04970.07410.02890.3172-0.00640.03820.34560.01860.43615.273132.71130.9565
180.2785-0.01690.01190.5606-0.20810.52490.0852-0.079-0.031-0.15010.28620.62290.35710.01950.17210.1825-0.01120.07750.39160.09060.37283.675534.536932.5128
190.0439-0.0067-0.01860.5423-0.1750.0570.3033-0.0779-0.08530.510.02180.4602-0.0064-0.00080.04340.373-0.00210.01190.4574-0.0260.248514.465941.285139.3147
200.0235-0.01430.0235-0.02570.02340.02730.04-0.18280.1496-0.1641-0.0113-0.1492-0.08150.31290.00020.56280.11150.08580.7174-0.02210.716216.297750.646441.0739
211.3959-0.61610.1831.6145-0.19180.67420.0127-0.4215-0.26350.23420.13090.1561-0.18140.09540.38060.2972-0.01860.0840.45990.04620.280210.291736.108844.8393
220.0799-0.05310.04010.072-0.03830.09980.11520.021-0.26520.38820.38190.1019-0.5038-0.153100.3931-0.09980.04690.46710.05850.6946-6.748748.327534.605
230.0044-0.04720.03440.097-0.07780.09140.0111-0.1376-0.00630.32440.0018-0.0966-0.5066-0.0523-0.00090.62410.06910.01160.42210.0460.6621-1.311453.457238.0507
240.2376-0.26060.02570.2682-0.07450.1760.16620.1314-0.05030.04410.06440.15950.1374-0.2598-0.0480.3897-0.0588-0.14460.46870.1320.9461-11.669935.350426.202
250.9879-0.001-0.4940.1265-0.03190.32-0.1543-0.3864-0.3945-0.49710.0133-0.103-0.14270.3593-0.04730.51730.0047-0.0669-0.0627-0.1340.5797-3.295849.712819.9182
260.00630.00160.00860.03510.0650.02860.5231-0.15640.36160.0552-0.2010.11440.35670.03640.00020.32220.29190.05870.49120.11340.51853.584746.739414.2221
270.0358-0.052-0.17310.09210.21080.57340.045-0.0239-0.0718-0.19990.02170.0381-0.13720.28540.01220.321-0.1071-0.0790.1901-0.01340.3387-12.594551.022415.64
280.09230.04930.0057-0.0033-0.03510.1636-0.29160.163-0.4186-0.3242-0.49290.25140.3670.1498-0.25620.4488-0.1205-0.2770.3243-0.20090.6725-8.424542.533110.8659
290.1950.07810.14821.08140.44732.0030.41130.366-0.1058-0.31270.39810.76040.046-0.01010.68260.4202-0.0403-0.16970.26330.13110.6263-13.841745.032318.9978
300.17180.0075-0.10040.03060.05410.1969-0.07650.1635-0.3124-0.1032-0.37720.46430.0696-0.066-0.15710.29120.1193-0.18340.2599-0.04521.0726-11.064432.775519.0481
310.1090.1116-0.26320.3077-0.30030.39230.1162-0.1546-0.1116-0.52810.4523-0.0174-0.543-0.66520.46490.20090.01430.20010.34590.16990.7346-12.363352.75526.8565
320.1067-0.0795-0.23950.19490.21960.9337-0.5386-0.2135-0.07940.1702-0.01780.185-0.1008-0.06-0.53630.3516-0.00710.00230.2714-0.02250.7611-4.487847.174132.7612
330.72370.31260.0260.14410.08010.44970.0592-0.48710.0639-0.04450.1425-0.10420.0427-0.0955-0.64790.43380.33640.4471.1673-0.43450.9219-22.626411.315826.912
340.718-0.6853-0.0660.59840.05810.0441-0.3002-0.2110.4815-0.1940.3048-0.23220.0708-0.40940.03590.3617-0.0829-0.060.52690.04230.2663-16.5197.833718.9929
350.1424-0.16140.02960.0596-0.01520.04610.03230.1887-0.0959-0.3630.1664-0.28610.30750.11340.19470.66920.0058-0.12120.3049-0.10190.3588-20.6988-1.011910.7804
360.01960.02610.01370.03110.01720.01320.2076-0.16620.250.0501-0.0437-0.1642-0.03790.04910.00020.53390.05660.14380.41810.06560.5615-14.41212.37292.8026
370.10250.03990.05710.10230.09620.1164-0.25820.00640.0765-0.2524-0.23090.13630.2004-0.4673-0.00140.89560.20290.13870.77160.0860.4347-16.4571-2.5485-6.4985
380.2503-0.40550.01160.66560.04060.07130.00230.0811-0.1554-0.046-0.0787-0.2150.02950.0094-0.06191.18110.33420.5220.65151.2699-0.3618-11.2376-2.9302-18.0506
390.10530.0020.10290.0258-0.01020.10010.2071-0.0376-0.3083-0.05960.0884-0.17150.2859-0.01930.02591.1868-0.35280.02881.1822-0.0370.9566-17.7511-9.5321-17.4654
400.0642-0.0475-0.01290.0840.13210.14830.0032-0.18880.0908-0.8907-0.0817-0.18920.252-0.02230.00050.8660.04310.09540.4770.03450.356519.485827.5681-14.4703
410.0635-0.1089-0.0936-0.00330.0695-0.06920.1015-0.15680.40490.10860.01130.4675-0.0112-0.1215-01.02950.0533-0.0411.45970.11241.2026-2.978538.1049-16.1261
420.6585-0.20730.00640.1279-0.18950.6104-0.6171-0.0798-0.18320.762-0.8545-0.63970.3407-0.0963-1.15490.38820.0777-0.15840.48570.95530.511932.33235.79145.6925
430.22180.03840.27020.38230.3790.8365-0.14840.10030.1084-0.0228-0.444-0.6499-0.20480.2304-0.75290.4151-0.0619-0.13050.72240.24740.616926.91842.033138.4224
44-0.005-0.0361-0.02370.03230.03360.01390.1259-0.15180.18080.5221-0.2571-0.462-0.2341-0.0186-0.00060.7594-0.0577-0.02190.71710.14210.548232.902150.947746.4565
450.034-0.0062-0.03660.01440.00650.0420.2963-0.33020.2706-0.2277-0.06160.2160.10310.00450.00680.61250.09780.03740.6982-0.04730.64226.391157.237539.2554
460.0274-0.0662-0.05660.15490.18020.2337-0.2952-0.1322-0.32880.10470.5828-0.3924-0.1768-0.0727-0.01780.76830.1413-0.03810.91630.12450.710328.326762.312443.4018
470.03190.03180.0070.0489-0.00390.0195-0.03910.0911-0.2468-0.05580.1433-0.3095-0.2875-0.19970.00130.72790.10760.07120.61690.19410.57329.27370.356438.2523
480.2859-0.1951-0.09110.13470.06010.0268-0.0264-0.19930.1660.0253-0.09810.0512-0.0677-0.0502-0.00230.77950.16390.10411.07170.30640.613723.472978.100435.9572
490.08560.0042-0.01640.009-0.00510.0001-0.0438-0.00850.01950.00390.03250.0266-0.0251-0.0609-01.57720.0787-0.04240.8589-0.15761.511626.498582.787141.4983
500.1211-0.0802-0.06380.20110.10.06960.0192-0.19790.0240.2678-0.0923-0.0299-0.1736-0.13220.04731.22640.0254-0.76141.25150.25551.217431.747578.77242.6939
510.12840.0575-0.0130.2044-0.09430.28150.54760.1944-0.0298-0.0121-0.2362-0.1058-0.45110.36730.00360.6458-0.06-0.02930.28180.02040.4718-6.815861.42279.6525
521.2526-0.08650.03050.0053-0.01050.25580.330.5656-0.8308-0.5759-0.30610.08270.5870.18620.00671.1302-0.0856-0.00110.9366-0.11861.3021.095356.5571-0.1309
530.02910.03810.02270.0747-0.03850.061-0.05350.0261-0.206-0.09630.0145-0.05670.13430.0251-0.02081.55840.39160.00592.00720.32151.841214.856555.83262.1505
54-0.0134-0.00490.0038-0.00180.0003-0.00270.0435-0.1143-0.04190.04020.0376-0.0176-0.00040.1382-01.39810.04730.18441.86550.1341.9320.346357.6768-0.8439
550.0036-0.0004-0.0029-0.0039-0.0008-0.00490.27790.01980.1867-0.0449-0.03060.01910.1065-0.06480.00041.7754-0.03010.68162.21290.23572.51529.22561.9827-4.2262
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1CHAIN A AND (RESID 57 THROUGH 93 )
2X-RAY DIFFRACTION2CHAIN A AND (RESID 94 THROUGH 100 )
3X-RAY DIFFRACTION3CHAIN A AND (RESID 101 THROUGH 113 )
4X-RAY DIFFRACTION4CHAIN A AND (RESID 114 THROUGH 157 )
5X-RAY DIFFRACTION5CHAIN A AND (RESID 158 THROUGH 197 )
6X-RAY DIFFRACTION6CHAIN A AND (RESID 198 THROUGH 208 )
7X-RAY DIFFRACTION7CHAIN B AND (RESID 54 THROUGH 68 )
8X-RAY DIFFRACTION8CHAIN B AND (RESID 69 THROUGH 83 )
9X-RAY DIFFRACTION9CHAIN B AND (RESID 84 THROUGH 93 )
10X-RAY DIFFRACTION10CHAIN B AND (RESID 94 THROUGH 106 )
11X-RAY DIFFRACTION11CHAIN B AND (RESID 107 THROUGH 125 )
12X-RAY DIFFRACTION12CHAIN B AND (RESID 126 THROUGH 138 )
13X-RAY DIFFRACTION13CHAIN B AND (RESID 139 THROUGH 157 )
14X-RAY DIFFRACTION14CHAIN B AND (RESID 158 THROUGH 184 )
15X-RAY DIFFRACTION15CHAIN B AND (RESID 185 THROUGH 197 )
16X-RAY DIFFRACTION16CHAIN B AND (RESID 198 THROUGH 211 )
17X-RAY DIFFRACTION17CHAIN C AND (RESID 57 THROUGH 68 )
18X-RAY DIFFRACTION18CHAIN C AND (RESID 69 THROUGH 93 )
19X-RAY DIFFRACTION19CHAIN C AND (RESID 94 THROUGH 106 )
20X-RAY DIFFRACTION20CHAIN C AND (RESID 107 THROUGH 119 )
21X-RAY DIFFRACTION21CHAIN C AND (RESID 120 THROUGH 211 )
22X-RAY DIFFRACTION22CHAIN D AND (RESID 56 THROUGH 68 )
23X-RAY DIFFRACTION23CHAIN D AND (RESID 69 THROUGH 84 )
24X-RAY DIFFRACTION24CHAIN D AND (RESID 85 THROUGH 93 )
25X-RAY DIFFRACTION25CHAIN D AND (RESID 94 THROUGH 106 )
26X-RAY DIFFRACTION26CHAIN D AND (RESID 107 THROUGH 119 )
27X-RAY DIFFRACTION27CHAIN D AND (RESID 120 THROUGH 132 )
28X-RAY DIFFRACTION28CHAIN D AND (RESID 133 THROUGH 148 )
29X-RAY DIFFRACTION29CHAIN D AND (RESID 149 THROUGH 168 )
30X-RAY DIFFRACTION30CHAIN D AND (RESID 169 THROUGH 178 )
31X-RAY DIFFRACTION31CHAIN D AND (RESID 179 THROUGH 197 )
32X-RAY DIFFRACTION32CHAIN D AND (RESID 198 THROUGH 209 )
33X-RAY DIFFRACTION33CHAIN E AND (RESID 36 THROUGH 45 )
34X-RAY DIFFRACTION34CHAIN E AND (RESID 46 THROUGH 76 )
35X-RAY DIFFRACTION35CHAIN E AND (RESID 77 THROUGH 92 )
36X-RAY DIFFRACTION36CHAIN E AND (RESID 93 THROUGH 101 )
37X-RAY DIFFRACTION37CHAIN E AND (RESID 102 THROUGH 126 )
38X-RAY DIFFRACTION38CHAIN E AND (RESID 127 THROUGH 131 )
39X-RAY DIFFRACTION39CHAIN E AND (RESID 132 THROUGH 145 )
40X-RAY DIFFRACTION40CHAIN F AND (RESID 40 THROUGH 76 )
41X-RAY DIFFRACTION41CHAIN F AND (RESID 77 THROUGH 142 )
42X-RAY DIFFRACTION42CHAIN G AND (RESID 39 THROUGH 60 )
43X-RAY DIFFRACTION43CHAIN G AND (RESID 61 THROUGH 76 )
44X-RAY DIFFRACTION44CHAIN G AND (RESID 77 THROUGH 92 )
45X-RAY DIFFRACTION45CHAIN G AND (RESID 93 THROUGH 101 )
46X-RAY DIFFRACTION46CHAIN G AND (RESID 102 THROUGH 111 )
47X-RAY DIFFRACTION47CHAIN G AND (RESID 112 THROUGH 126 )
48X-RAY DIFFRACTION48CHAIN G AND (RESID 127 THROUGH 131 )
49X-RAY DIFFRACTION49CHAIN G AND (RESID 132 THROUGH 136 )
50X-RAY DIFFRACTION50CHAIN G AND (RESID 137 THROUGH 145 )
51X-RAY DIFFRACTION51CHAIN H AND (RESID 39 THROUGH 76 )
52X-RAY DIFFRACTION52CHAIN H AND (RESID 77 THROUGH 96 )
53X-RAY DIFFRACTION53CHAIN H AND (RESID 97 THROUGH 106 )
54X-RAY DIFFRACTION54CHAIN H AND (RESID 107 THROUGH 126 )
55X-RAY DIFFRACTION55CHAIN H AND (RESID 127 THROUGH 143 )

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