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- PDB-4c0s: Mammalian translation elongation factor eEF1A2 -

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Basic information

Entry
Database: PDB / ID: 4c0s
TitleMammalian translation elongation factor eEF1A2
ComponentsELONGATION FACTOR 1-ALPHA 2
KeywordsTRANSLATION
Function / homology
Function and homology information


translation elongation factor activity / GTPase activity / GTP binding / nucleus
Similarity search - Function
Translation elongation factor EF1A, eukaryotic/archaeal / Translation elongation factor EFTu/EF1A, C-terminal / Elongation factor Tu C-terminal domain / Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal / Translation factors / Tr-type G domain, conserved site / Translational (tr)-type guanine nucleotide-binding (G) domain signature. / Translation elongation factor EFTu-like, domain 2 / Elongation factor Tu domain 2 / Translational (tr)-type GTP-binding domain ...Translation elongation factor EF1A, eukaryotic/archaeal / Translation elongation factor EFTu/EF1A, C-terminal / Elongation factor Tu C-terminal domain / Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal / Translation factors / Tr-type G domain, conserved site / Translational (tr)-type guanine nucleotide-binding (G) domain signature. / Translation elongation factor EFTu-like, domain 2 / Elongation factor Tu domain 2 / Translational (tr)-type GTP-binding domain / Elongation factor Tu GTP binding domain / Translational (tr)-type guanine nucleotide-binding (G) domain profile. / Elongation Factor Tu (Ef-tu); domain 3 / Translation protein, beta-barrel domain superfamily / P-loop containing nucleotide triphosphate hydrolases / Beta Barrel / P-loop containing nucleoside triphosphate hydrolase / Rossmann fold / 3-Layer(aba) Sandwich / Mainly Beta / Alpha Beta
Similarity search - Domain/homology
GUANOSINE-5'-DIPHOSPHATE / Elongation factor 1-alpha 2
Similarity search - Component
Biological speciesORYCTOLAGUS CUNICULUS (rabbit)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MIR / Resolution: 2.703 Å
AuthorsCrepin, T. / Shalak, V.F. / Yaremchuk, A.D. / Vlasenko, D.O. / McCarthy, A.A. / Negrutskii, B.S. / Tukalo, M.A. / El'skaya, A.V.
CitationJournal: Nucleic Acids Res. / Year: 2014
Title: Mammalian Translation Elongation Factor Eef1A2: X-Ray Structure and New Features of Gdp/GTP Exchange Mechanism in Higher Eukaryotes
Authors: Crepin, T. / Mccarthy, A. / Negrutskii, A. / Shalak, V.F. / Tukalo, V. / Vlasenko, D.O. / Yaremchuk, A.D.
History
DepositionAug 7, 2013Deposition site: PDBE / Processing site: PDBE
Revision 1.0Aug 27, 2014Provider: repository / Type: Initial release
Revision 1.1Oct 29, 2014Group: Database references
Revision 1.2Nov 26, 2014Group: Database references
Revision 1.3Jan 25, 2017Group: Data collection
Remark 700 SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AC" IN EACH CHAIN ON SHEET RECORDS BELOW ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AC" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 6-STRANDED BARREL THIS IS REPRESENTED BY A 7-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEETS PRESENTED AS "AD" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 6-STRANDED BARREL THIS IS REPRESENTED BY A 7-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEETS PRESENTED AS "BC" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 6-STRANDED BARREL THIS IS REPRESENTED BY A 7-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEETS PRESENTED AS "BD" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN -9-STRANDED BARREL THIS IS REPRESENTED BY A -8-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL.

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: ELONGATION FACTOR 1-ALPHA 2
B: ELONGATION FACTOR 1-ALPHA 2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)102,3456
Polymers101,4102
Non-polymers9354
Water1629
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area6310 Å2
ΔGint-28.5 kcal/mol
Surface area35990 Å2
MethodPISA
Unit cell
Length a, b, c (Å)135.370, 135.370, 304.620
Angle α, β, γ (deg.)90.00, 90.00, 120.00
Int Tables number178
Space group name H-MP6122

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Components

#1: Protein ELONGATION FACTOR 1-ALPHA 2 / EF-1-ALPHA-2 / EUKARYOTIC ELONGATION FACTOR 1 A-2 / EEF1A-2 / STATIN-S1


Mass: 50705.059 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ORYCTOLAGUS CUNICULUS (rabbit) / Tissue: MUSCLESkeletal muscle / References: UniProt: Q71V39
#2: Chemical ChemComp-GDP / GUANOSINE-5'-DIPHOSPHATE / Guanosine diphosphate


Type: RNA linking / Mass: 443.201 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C10H15N5O11P2 / Comment: GDP, energy-carrying molecule*YM
#3: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Mg
#4: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 9 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 10

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Sample preparation

CrystalDensity Matthews: 3.87 Å3/Da / Density % sol: 68.1 % / Description: NONE
Crystal growDetails: 50 MM MES PH 5.6, 2.4 M AMMONIUM SULFATE, 10 MM MAGNESIUM ACETATE

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: ESRF / Beamline: ID14-4 / Wavelength: 0.97936
DetectorType: ADSC CCD / Detector: CCD / Date: Dec 18, 2010
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97936 Å / Relative weight: 1
ReflectionResolution: 2.7→25 Å / Num. obs: 45528 / % possible obs: 98.4 % / Observed criterion σ(I): 2.5 / Redundancy: 7.5 % / Rmerge(I) obs: 0.08 / Net I/σ(I): 18.52
Reflection shellResolution: 2.7→2.8 Å / Redundancy: 7.7 % / Rmerge(I) obs: 0.86 / Mean I/σ(I) obs: 2.76 / % possible all: 95.6

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Processing

Software
NameVersionClassification
REFMAC5.6.0114refinement
XDSdata reduction
XSCALEdata scaling
RefinementMethod to determine structure: MIR
Starting model: NONE

Resolution: 2.703→25 Å / Cor.coef. Fo:Fc: 0.947 / Cor.coef. Fo:Fc free: 0.916 / SU B: 11.093 / SU ML: 0.22 / Cross valid method: THROUGHOUT / σ(F): 2.703 / ESU R: 0.374 / ESU R Free: 0.283 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
RfactorNum. reflection% reflection
Rfree0.2556 2302 5.16 %
Rwork0.2019 --
obs0.205 45527 98.972 %
Solvent computationIon probe radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK BULK SOLVENT
Displacement parametersBiso mean: 62.295 Å2
Baniso -1Baniso -2Baniso -3
1-1.623 Å20.811 Å20 Å2
2--1.623 Å20 Å2
3----2.434 Å2
Refinement stepCycle: LAST / Resolution: 2.703→25 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms6884 0 58 9 6951
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0150.0197085
X-RAY DIFFRACTIONr_bond_other_d
X-RAY DIFFRACTIONr_angle_refined_deg1.5121.9839601
X-RAY DIFFRACTIONr_angle_other_deg
X-RAY DIFFRACTIONr_dihedral_angle_1_deg8.965890
X-RAY DIFFRACTIONr_dihedral_angle_2_deg39.33524.42276
X-RAY DIFFRACTIONr_dihedral_angle_3_deg22.713151255
X-RAY DIFFRACTIONr_dihedral_angle_4_deg19.9191536
X-RAY DIFFRACTIONr_chiral_restr0.1170.21075
X-RAY DIFFRACTIONr_gen_planes_refined0.010.0215210
X-RAY DIFFRACTIONr_gen_planes_other
X-RAY DIFFRACTIONr_nbd_refined0.2630.23366
X-RAY DIFFRACTIONr_nbd_other
X-RAY DIFFRACTIONr_nbtor_refined0.3310.24774
X-RAY DIFFRACTIONr_nbtor_other
X-RAY DIFFRACTIONr_xyhbond_nbd_refined0.1720.2263
X-RAY DIFFRACTIONr_xyhbond_nbd_other
X-RAY DIFFRACTIONr_metal_ion_refined
X-RAY DIFFRACTIONr_metal_ion_other
X-RAY DIFFRACTIONr_symmetry_vdw_refined
X-RAY DIFFRACTIONr_symmetry_vdw_other
X-RAY DIFFRACTIONr_symmetry_hbond_refined
X-RAY DIFFRACTIONr_symmetry_hbond_other
X-RAY DIFFRACTIONr_symmetry_metal_ion_refined
X-RAY DIFFRACTIONr_symmetry_metal_ion_other
X-RAY DIFFRACTIONr_mcbond_it6.3066.1827084
X-RAY DIFFRACTIONr_mcbond_other
X-RAY DIFFRACTIONr_mcangle_it9.3339.1569600
X-RAY DIFFRACTIONr_mcangle_other
X-RAY DIFFRACTIONr_scbond_it14.17218.8932457
X-RAY DIFFRACTIONr_scbond_other
X-RAY DIFFRACTIONr_scangle_it12.24129.4568281
X-RAY DIFFRACTIONr_scangle_other
X-RAY DIFFRACTIONr_long_range_B_refined
X-RAY DIFFRACTIONr_long_range_B_other
X-RAY DIFFRACTIONr_rigid_bond_restr
X-RAY DIFFRACTIONr_sphericity_free
X-RAY DIFFRACTIONr_sphericity_bonded
LS refinement shellResolution: 2.703→2.772 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.422 144 -
Rwork0.344 2806 -
obs--98.761 %

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