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Yorodumi- PDB-4c0m: Crystal Structure of the N terminal domain of wild type TRIF (TIR... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4c0m | ||||||
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Title | Crystal Structure of the N terminal domain of wild type TRIF (TIR- domain-containing adapter-inducing interferon-beta) | ||||||
Components | TIR DOMAIN-CONTAINING ADAPTER MOLECULE 1 | ||||||
Keywords | IMMUNE SYSTEM / TOLL-LIKE RECEPTOR ADAPTOR PROTEIN / INNATE IMMUNITY / TETRATRICO-PEPTIDE REPEAT (TPR) / IFIT PROTEINS | ||||||
Function / homology | Function and homology information TICAM1 deficiency - HSE / TRAF3 deficiency - HSE / ripoptosome / MyD88-independent TLR4 cascade / Toll Like Receptor 3 (TLR3) Cascade / TRIF-mediated programmed cell death / TLR3-mediated TICAM1-dependent programmed cell death / positive regulation of myeloid dendritic cell cytokine production / cellular response to oxidised low-density lipoprotein particle stimulus / Caspase activation via Death Receptors in the presence of ligand ...TICAM1 deficiency - HSE / TRAF3 deficiency - HSE / ripoptosome / MyD88-independent TLR4 cascade / Toll Like Receptor 3 (TLR3) Cascade / TRIF-mediated programmed cell death / TLR3-mediated TICAM1-dependent programmed cell death / positive regulation of myeloid dendritic cell cytokine production / cellular response to oxidised low-density lipoprotein particle stimulus / Caspase activation via Death Receptors in the presence of ligand / toll-like receptor 3 signaling pathway / TRIF-dependent toll-like receptor signaling pathway / RIP-mediated NFkB activation via ZBP1 / macrophage activation involved in immune response / positive regulation of cytokine production involved in inflammatory response / positive regulation of natural killer cell activation / positive regulation of macrophage cytokine production / toll-like receptor 4 signaling pathway / toll-like receptor signaling pathway / response to exogenous dsRNA / B cell proliferation / regulation of protein-containing complex assembly / autophagosome / positive regulation of type I interferon production / signaling adaptor activity / positive regulation of autophagy / positive regulation of B cell proliferation / positive regulation of chemokine production / lipopolysaccharide-mediated signaling pathway / IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation / TICAM1,TRAF6-dependent induction of TAK1 complex / TICAM1-dependent activation of IRF3/IRF7 / TRAF6-mediated induction of TAK1 complex within TLR4 complex / nitric oxide biosynthetic process / positive regulation of interferon-beta production / TICAM1, RIP1-mediated IKK complex recruitment / Activation of IRF3, IRF7 mediated by TBK1, IKKε (IKBKE) / IKK complex recruitment mediated by RIP1 / positive regulation of protein ubiquitination / apoptotic signaling pathway / positive regulation of interleukin-6 production / positive regulation of nitric oxide biosynthetic process / positive regulation of tumor necrosis factor production / defense response to virus / positive regulation of canonical NF-kappaB signal transduction / cellular response to lipopolysaccharide / molecular adaptor activity / early endosome / endosome membrane / endosome / inflammatory response / innate immune response / positive regulation of gene expression / protein kinase binding / mitochondrion / cytosol Similarity search - Function | ||||||
Biological species | HOMO SAPIENS (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.8 Å | ||||||
Authors | Ullah, M.O. / Ve, T. / Mangan, M. / Alaidarous, M. / Sweet, M.J. / Mansell, A. / Kobe, B. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 2013 Title: The Tlr Signalling Adaptor Trif/Ticam-1 Has an N-Terminal Helical Domain with Structural Similarity to Ifit Proteins Authors: Ullah, M.O. / Ve, T. / Mangan, M. / Alaidarous, M. / Sweet, M.J. / Mansell, A. / Kobe, B. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4c0m.cif.gz | 218.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4c0m.ent.gz | 178.3 KB | Display | PDB format |
PDBx/mmJSON format | 4c0m.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/c0/4c0m ftp://data.pdbj.org/pub/pdb/validation_reports/c0/4c0m | HTTPS FTP |
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-Related structure data
Related structure data | 4bsxSC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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3 |
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4 |
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Unit cell |
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-Components
#1: Protein | Mass: 17021.229 Da / Num. of mol.: 4 / Fragment: N-TERMINAL DOMAIN, RESIDUES 1-153 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Plasmid: PMCSG7 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): B834 / References: UniProt: Q8IUC6 |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.26 Å3/Da / Density % sol: 45.7 % / Description: NONE |
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Crystal grow | Details: 0.1 M BIS TRIS PH 6.6, 150 MM NACL AND 21% PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Australian Synchrotron / Beamline: MX2 / Wavelength: 0.95369 |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Mar 15, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.95369 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→44.51 Å / Num. obs: 13337 / % possible obs: 89.6 % / Observed criterion σ(I): 2 / Redundancy: 1.8 % / Biso Wilson estimate: 34.21 Å2 / Rmerge(I) obs: 0.2 / Net I/σ(I): 3.9 |
Reflection shell | Resolution: 2.8→2.95 Å / Redundancy: 1.8 % / Rmerge(I) obs: 0.4 / Mean I/σ(I) obs: 2 / % possible all: 90.4 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 4BSX Resolution: 2.8→39.7 Å / Cor.coef. Fo:Fc: 0.8277 / Cor.coef. Fo:Fc free: 0.7729 / Cross valid method: THROUGHOUT / σ(F): 0 / SU Rfree Blow DPI: 0.458
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Displacement parameters | Biso mean: 22.66 Å2
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Refine analyze | Luzzati coordinate error obs: 0.48 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.8→39.7 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.8→3.02 Å / Total num. of bins used: 7
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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