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Yorodumi- PDB-1hsy: ORIGIN OF THE PH-DEPENDENT SPECTROSCOPIC PROPERTIES OF PENTACOORD... -
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-Basic information
Entry | Database: PDB / ID: 1hsy | ||||||
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Title | ORIGIN OF THE PH-DEPENDENT SPECTROSCOPIC PROPERTIES OF PENTACOORDINATE METMYOGLOBIN VARIANTS | ||||||
Components | MYOGLOBIN | ||||||
Keywords | OXYGEN TRANSPORT | ||||||
Function / homology | Function and homology information nitrite reductase activity / Oxidoreductases; Acting on other nitrogenous compounds as donors / oxygen transport / sarcoplasm / Oxidoreductases; Acting on a peroxide as acceptor; Peroxidases / removal of superoxide radicals / oxygen carrier activity / oxygen binding / peroxidase activity / heme binding ...nitrite reductase activity / Oxidoreductases; Acting on other nitrogenous compounds as donors / oxygen transport / sarcoplasm / Oxidoreductases; Acting on a peroxide as acceptor; Peroxidases / removal of superoxide radicals / oxygen carrier activity / oxygen binding / peroxidase activity / heme binding / extracellular exosome / metal ion binding Similarity search - Function | ||||||
Biological species | Equus caballus (horse) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.9 Å | ||||||
Authors | Maurus, R. / Brayer, G.D. | ||||||
Citation | Journal: Biochemistry / Year: 1995 Title: Origin of the pH-dependent spectroscopic properties of pentacoordinate metmyoglobin variants. Authors: Bogumil, R. / Maurus, R. / Hildebrand, D.P. / Brayer, G.D. / Mauk, A.G. #1: Journal: To be Published Title: Thermal Destabilization of Horse Heart Myoglobin Through Modification of a Hydrophobic Cluster in the Proximal Heme Pocket Authors: Maurus, R. / Overall, C.M. / Bogumil, R. / Luo, Y. / Mauk, A.G. / Smith, M. / Brayer, G.D. #2: Journal: J.Biol.Chem. / Year: 1994 Title: Structural Characterization of Heme Ligation in the His64->Tyr Variant of Myoglobin Authors: Maurus, R. / Bogumil, R. / Luo, Y. / Tang, H.L. / Smith, M. / Mauk, A.G. / Brayer, G.D. #3: Journal: Biochemistry / Year: 1994 Title: Ftir Analysis of the Interaction of Azide with Horse Heart Myoglobin Authors: Bogumil, R. / Hunter, C.L. / Maurus, R. / Tang, H.L. / Lee, H. / Lloyd, E. / Brayer, G.D. / Smith, M. / Mauk, A.G. #4: Journal: Proc.Natl.Acad.Sci.USA / Year: 1994 Title: Three-Dimensional Structure of Cyanomet-Sulfmyoglobin C Authors: Evans, S.V. / Sishta, B.P. / Mauk, A.G. / Brayer, G.D. #5: Journal: Protein Eng. / Year: 1991 Title: Expression in Escherichia Coli of a Synthetic Gene Coding for Horse Heart Myoglobin Authors: Guillemette, J.G. / Matsushima-Hibiya, Y. / Atkinson, T. / Smith, M. #6: Journal: J.Mol.Biol. / Year: 1990 Title: High-Resolution Study of the Three-Dimensional Structure of Horse Heart Myoglobin Authors: Evans, S.V. / Brayer, G.D. #7: Journal: J.Biol.Chem. / Year: 1988 Title: Horse Heart Metmyoglobin: A 2.8-Angstroms Resolution Three-Dimensional Structure Determination Authors: Evans, S.V. / Brayer, G.D. #8: Journal: J.Mol.Biol. / Year: 1987 Title: Crystallization and Preliminary Diffraction Data for Horse Heart Metmyoglobin Authors: Sherwood, C. / Mauk, A.G. / Brayer, G.D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1hsy.cif.gz | 44.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1hsy.ent.gz | 31.2 KB | Display | PDB format |
PDBx/mmJSON format | 1hsy.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1hsy_validation.pdf.gz | 482.1 KB | Display | wwPDB validaton report |
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Full document | 1hsy_full_validation.pdf.gz | 488.2 KB | Display | |
Data in XML | 1hsy_validation.xml.gz | 5.8 KB | Display | |
Data in CIF | 1hsy_validation.cif.gz | 8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hs/1hsy ftp://data.pdbj.org/pub/pdb/validation_reports/hs/1hsy | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 16946.471 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Equus caballus (horse) / Gene: SYNTHETIC GENE / Organ: HEART / References: UniProt: P68082 |
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#2: Chemical | ChemComp-SO4 / |
#3: Chemical | ChemComp-HEM / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 1.87 Å3/Da / Density % sol: 34.37 % | ||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 8.4 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | Num. obs: 7270 / % possible obs: 68.2 % / Observed criterion σ(I): 2 |
Reflection | *PLUS Highest resolution: 1.9 Å / Num. measured all: 86121 / Rmerge(I) obs: 0.086 |
-Processing
Software | Name: PROLSQ / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Resolution: 1.9→6 Å / σ(F): 2 /
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Displacement parameters | Biso mean: 15.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→6 Å
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Refine LS restraints |
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