A: VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 33A B: VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 33A C: VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 16 HOMOLOG ヘテロ分子
ADDITIONAL C-TERMINAL RESIDUES ARE DERIVED FROM THE EXPRESSION VECTOR. ADDITIONAL N-TERMINAL ...ADDITIONAL C-TERMINAL RESIDUES ARE DERIVED FROM THE EXPRESSION VECTOR. ADDITIONAL N-TERMINAL RESIDUES ARE RESIDUAL FROM CLEAVAGE OF GST PURIFICATION TAG
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 3.64 Å3/Da / 溶媒含有率: 66.19 % / 解説: NONE
結晶化
温度: 293 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 6 詳細: CRYSTALS WERE GROWN IN SITTING DROPS AT 20C. VPS33A:VPS16 COMPLEX (200 NL; A280 = 7.13, APPROX. 8.7 MG/ML) WAS MIXED 1:1 WITH RESERVOIR SOLUTION AND EQUILIBRATED AGAINST 80 UL RESERVOIRS ...詳細: CRYSTALS WERE GROWN IN SITTING DROPS AT 20C. VPS33A:VPS16 COMPLEX (200 NL; A280 = 7.13, APPROX. 8.7 MG/ML) WAS MIXED 1:1 WITH RESERVOIR SOLUTION AND EQUILIBRATED AGAINST 80 UL RESERVOIRS CONTAINING 4% TACSIMATE PH 6.0, 12% PEG 3350 (HAMPTON RESEARCH). CRYSTALS WERE CRYOPROTECTED BY BRIEF IMMERSION IN RESERVOIR SOLUTION SUPPLEMENTED WITH 30% ETHYLENE GLYCOL AND CRYO-COOLED BY PLUNGING INTO LIQUID NITROGEN.
解像度: 2.6→85.84 Å / Cor.coef. Fo:Fc: 0.963 / Cor.coef. Fo:Fc free: 0.943 / SU B: 15.437 / SU ML: 0.158 / 交差検証法: THROUGHOUT / ESU R: 0.302 / ESU R Free: 0.22 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES WITH TLS ADDED
Rfactor
反射数
%反射
Selection details
Rfree
0.21024
3465
5.1 %
RANDOM
Rwork
0.17594
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obs
0.17762
65003
99.98 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK