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- PDB-4bof: Crystal structure of arginine deiminase from group A streptococcus -

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Open data


ID or keywords:

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Basic information

Entry
Database: PDB / ID: 4bof
TitleCrystal structure of arginine deiminase from group A streptococcus
ComponentsARGININE DEIMINASE
KeywordsHYDROLASE / VACCINE
Function / homology
Function and homology information


arginine deiminase / arginine deiminase activity / arginine catabolic process to ornithine / cytoplasm
Similarity search - Function
Pentein / Arginine deiminase / Arginine deiminase / Arginine deiminase / L-arginine/glycine Amidinotransferase; Chain A / 5-stranded Propeller / L-arginine/glycine Amidinotransferase; Chain A / Orthogonal Bundle / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
TRIETHYLENE GLYCOL / Arginine deiminase
Similarity search - Component
Biological speciesSTREPTOCOCCUS PYOGENES (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.48 Å
AuthorsHenningham, A. / Ericsson, D.J. / Langer, K. / Casey, L. / Jovcevski, B. / Chhatwal, G.S. / Aquilina, J.A. / Batzloff, M.R. / Kobe, B. / Walker, M.J.
CitationJournal: MBio / Year: 2013
Title: Structure-informed design of an enzymatically inactive vaccine component for group A Streptococcus.
Authors: Henningham, A. / Ericsson, D.J. / Langer, K. / Casey, L.W. / Jovcevski, B. / Chhatwal, G.S. / Aquilina, J.A. / Batzloff, M.R. / Kobe, B. / Walker, M.J.
History
DepositionMay 20, 2013Deposition site: PDBE / Processing site: PDBE
Revision 1.0Aug 21, 2013Provider: repository / Type: Initial release
Revision 1.1Aug 28, 2013Group: Derived calculations
Revision 1.2Feb 27, 2019Group: Data collection / Database references / Other
Category: citation / pdbx_database_proc / pdbx_database_status
Item: _citation.journal_abbrev / _citation.pdbx_database_id_DOI ..._citation.journal_abbrev / _citation.pdbx_database_id_DOI / _citation.title / _pdbx_database_status.recvd_author_approval
Revision 1.3Dec 20, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Other / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_database_status / pdbx_initial_refinement_model / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.status_code_sf / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: ARGININE DEIMINASE
B: ARGININE DEIMINASE
C: ARGININE DEIMINASE
D: ARGININE DEIMINASE
E: ARGININE DEIMINASE
F: ARGININE DEIMINASE
G: ARGININE DEIMINASE
H: ARGININE DEIMINASE
hetero molecules


Theoretical massNumber of molelcules
Total (without water)372,85327
Polymers370,8218
Non-polymers2,03219
Water5,242291
1
A: ARGININE DEIMINASE
H: ARGININE DEIMINASE
hetero molecules


Theoretical massNumber of molelcules
Total (without water)93,2407
Polymers92,7052
Non-polymers5345
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3850 Å2
ΔGint-64.2 kcal/mol
Surface area32030 Å2
MethodPISA
2
B: ARGININE DEIMINASE
C: ARGININE DEIMINASE
hetero molecules


Theoretical massNumber of molelcules
Total (without water)93,0906
Polymers92,7052
Non-polymers3844
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3420 Å2
ΔGint-71.8 kcal/mol
Surface area32160 Å2
MethodPISA
3
F: ARGININE DEIMINASE
G: ARGININE DEIMINASE
hetero molecules


Theoretical massNumber of molelcules
Total (without water)93,4348
Polymers92,7052
Non-polymers7296
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4490 Å2
ΔGint-59.8 kcal/mol
Surface area31650 Å2
MethodPISA
4
E: ARGININE DEIMINASE
hetero molecules

D: ARGININE DEIMINASE
hetero molecules


Theoretical massNumber of molelcules
Total (without water)93,0906
Polymers92,7052
Non-polymers3844
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation1_554x,y,z-11
Buried area3450 Å2
ΔGint-74.2 kcal/mol
Surface area31710 Å2
MethodPISA
5
D: ARGININE DEIMINASE
hetero molecules

E: ARGININE DEIMINASE
hetero molecules


Theoretical massNumber of molelcules
Total (without water)93,0906
Polymers92,7052
Non-polymers3844
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation1_556x,y,z+11
Buried area3450 Å2
ΔGint-74.2 kcal/mol
Surface area31710 Å2
MethodPISA
Unit cell
Length a, b, c (Å)87.910, 92.740, 120.970
Angle α, β, γ (deg.)96.15, 90.27, 100.13
Int Tables number1
Space group name H-MP1
Noncrystallographic symmetry (NCS)NCS oper:
IDCodeMatrixVector
1given(0.3872, -0.3496, -0.8531), (0.8175, 0.5581, 0.1424), (0.4264, -0.7526, 0.5019)30.26, -10.98, -52.81
2given(-0.6112, 0.3619, 0.7039), (0.4908, -0.5244, 0.6958), (0.6209, 0.7708, 0.1429)-20.58, -56.84, -6.436
3given(-0.563, 0.5608, -0.607), (0.5538, -0.2892, -0.7808), (-0.6135, -0.7758, -0.1478)36.15, 7.079, -29.1
4given(0.5213, -0.3511, 0.7778), (-0.3566, -0.9177, -0.1752), (0.7753, -0.186, -0.6036)62.76, -39.91, -66.92
5given(0.2358, -0.9665, -0.1012), (-0.9665, -0.2441, 0.0797), (-0.1017, 0.079, -0.9917)-20.99, -23.77, -30.21
6given(-0.03091, 0.9661, 0.2565), (-0.3555, 0.2292, -0.9062), (-0.9342, -0.1192, 0.3363)27.3, -26.69, -17.05
7given(-0.9647, -0.1933, -0.1791), (-0.2203, 0.2189, 0.9505), (-0.1445, 0.9564, -0.2538)-3.122, 2.91, -4.74

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Components

#1: Protein
ARGININE DEIMINASE / / ADI / ARGININE DIHYDROLASE / AD / STREPTOCOCCAL ACID GLYCOPROTEIN


Mass: 46352.676 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) STREPTOCOCCUS PYOGENES (bacteria) / Strain: MGAS5005 / Description: GROUP A STREPTOCOCCUS / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P0C0B3, arginine deiminase
#2: Chemical
ChemComp-SO4 / SULFATE ION / Sulfate


Mass: 96.063 Da / Num. of mol.: 16 / Source method: obtained synthetically / Formula: SO4
#3: Chemical ChemComp-PGE / TRIETHYLENE GLYCOL / Polyethylene glycol


Mass: 150.173 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C6H14O4
#4: Chemical ChemComp-PG4 / TETRAETHYLENE GLYCOL / Polyethylene glycol


Mass: 194.226 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C8H18O5 / Comment: precipitant*YM
#5: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 291 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.62 Å3/Da / Density % sol: 53 %
Description: DATA INTEGRATED FROM TWO LATTICES IN A SINGLE CRYSTAL.
Crystal growpH: 5.5
Details: 0.1M BIS-TRIS PH 5.5, 20% PEG3350, 0.3M LISO4, 25MG/ML PROTEIN

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: Australian Synchrotron / Beamline: MX1 / Wavelength: 0.953693
DetectorType: ADSC QUANTUM 210r / Detector: CCD / Date: Dec 14, 2011
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.953693 Å / Relative weight: 1
ReflectionResolution: 2.48→46.85 Å / Num. obs: 131658 / % possible obs: 99.3 % / Observed criterion σ(I): 1.21 / Redundancy: 7.4 % / Biso Wilson estimate: 54.68 Å2 / Rmerge(I) obs: 0.15 / Net I/σ(I): 10.25
Reflection shellResolution: 2.48→2.57 Å / Redundancy: 3.2 % / Rmerge(I) obs: 0.98 / Mean I/σ(I) obs: 1.21 / % possible all: 94.1

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Processing

Software
NameVersionClassification
BUSTER2.10.0refinement
XDSdata reduction
XSCALEdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 1S9R

1s9r


Resolution: 2.48→36.31 Å / Cor.coef. Fo:Fc: 0.924 / Cor.coef. Fo:Fc free: 0.9033 / SU R Cruickshank DPI: 0.523 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.453 / SU Rfree Blow DPI: 0.258 / SU Rfree Cruickshank DPI: 0.271
RfactorNum. reflection% reflectionSelection details
Rfree0.2441 6579 5 %RANDOM
Rwork0.2111 ---
obs0.2128 131631 99.34 %-
Displacement parametersBiso mean: 58.69 Å2
Baniso -1Baniso -2Baniso -3
1--6.9793 Å28.2544 Å23.0916 Å2
2---0.8683 Å21.0706 Å2
3---7.8477 Å2
Refine analyzeLuzzati coordinate error obs: 0.415 Å
Refinement stepCycle: LAST / Resolution: 2.48→36.31 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms25915 0 113 291 26319
Refine LS restraints
Refine-IDTypeDev idealNumberRestraint functionWeight
X-RAY DIFFRACTIONt_bond_d0.0126481HARMONIC2
X-RAY DIFFRACTIONt_angle_deg1.1235804HARMONIC2
X-RAY DIFFRACTIONt_dihedral_angle_d12528SINUSOIDAL2
X-RAY DIFFRACTIONt_incorr_chiral_ct
X-RAY DIFFRACTIONt_pseud_angle
X-RAY DIFFRACTIONt_trig_c_planes759HARMONIC2
X-RAY DIFFRACTIONt_gen_planes3772HARMONIC5
X-RAY DIFFRACTIONt_it26481HARMONIC20
X-RAY DIFFRACTIONt_nbd
X-RAY DIFFRACTIONt_omega_torsion3.15
X-RAY DIFFRACTIONt_other_torsion3.22
X-RAY DIFFRACTIONt_improper_torsion
X-RAY DIFFRACTIONt_chiral_improper_torsion3460SEMIHARMONIC5
X-RAY DIFFRACTIONt_sum_occupancies
X-RAY DIFFRACTIONt_utility_distance
X-RAY DIFFRACTIONt_utility_angle
X-RAY DIFFRACTIONt_utility_torsion
X-RAY DIFFRACTIONt_ideal_dist_contact29781SEMIHARMONIC4
LS refinement shellResolution: 2.48→2.54 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.2984 462 5.05 %
Rwork0.2414 8689 -
all0.2441 9151 -
obs--99.34 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.777-0.38130.17910.88760.33532.29480.1070.15690.12410.0879-0.0965-0.1404-0.45540.1259-0.0105-0.1156-0.05840.05380.04430.021-0.115823.1637-24.5151-42.8599
21.30780.430.16341.21380.18652.6348-0.05440.19880.1852-0.1704-0.06830.2223-0.0283-0.1530.1227-0.10510.05210.03180.0133-0.0019-0.09627.335-28.3162-55.3135
31.40760.6801-0.21740.5591-0.13192.3334-0.09620.28570.0445-0.24410.06730.0792-0.09660.09130.0289-0.0905-0.01050.02950.080.0092-0.040920.5895-31.9407-54.6794
41.951-0.62890.62841.98850.9364.24520.1708-0.0897-0.48190.18070.0196-0.44810.41120.7716-0.1904-0.19310.02340.02490.102-0.0491-0.039836.8706-41.2922-42.2418
50.1712-0.0337-0.0820.5207-0.37552.30550.0466-0.0915-0.10210.2307-0.06580.02070.2270.10640.0192-0.0879-0.06110.03810.0674-0.0086-0.123121.2898-35.6123-31.0353
61.3943-0.2537-0.10180.01790.81013.2179-0.19090.2262-0.17540.01060.1121-0.3371-0.13520.21460.0788-0.102-0.21980.0341-0.0156-0.0463-0.2233-18.4991-12.290714.974
72.7997-0.32680.57021.95640.16411.6318-0.45380.05580.7241-0.21220.2015-0.0358-1.03290.24910.25230.1693-0.1286-0.1547-0.1703-0.0492-0.1743-24.08191.897414.5883
82.4484-0.92120.43173.91471.30243.3396-0.12190.6787-0.2364-0.3155-0.09680.1994-0.62940.25190.2187-0.2469-0.20510.06710.1407-0.1103-0.2132-18.8545-18.1742-2.8736
90.1834-0.3549-1.0327-0.0134-0.85253.01430.05690.224-0.1379-0.09470.0943-0.2070.24690.3105-0.1512-0.2178-0.020.03620.1643-0.21010.049-15.9787-34.25856.4138
100.22480.247-0.30980.0942-0.32453.9025-0.05260.1593-0.29290.08120.1016-0.23960.14040.6378-0.049-0.2734-0.03120.05820.151-0.1067-0.0372-12.1488-25.212315.3838
111.5573-0.0869-2.33221.04550.43533.71650.1736-0.1899-0.21690.1939-0.1139-0.18860.1196-0.0947-0.0597-0.1332-0.0641-0.036-0.03880.0052-0.0776-31.7525-29.734237.1672
121.58750.91660.72352.79490.93710.37760.24540.1419-0.5525-0.0132-0.0662-0.1310.31720.1829-0.1792-0.08830.0023-0.0605-0.155-0.04210.0177-34.6166-45.427133.0138
131.7101-1.1102-0.10282.88541.47272.68960.1853-0.1401-0.34540.0868-0.11750.00140.149-0.0863-0.0678-0.0627-0.1357-0.0054-0.0320.0374-0.1099-44.9083-31.262443.0363
144.30260.7566-0.22342.1386-0.08170.10590.0541-0.04410.40730.00540.02890.3587-0.1148-0.2605-0.0830.0243-0.11320.04720.0254-0.0259-0.0983-49.3916-13.544246.0135
150.47210.39350.46172.7269-0.00510.90440.1554-0.0745-0.00560.0625-0.06380.0878-0.14450.2872-0.0915-0.0657-0.08560.03460.0355-0.0319-0.1108-30.5169-15.390740.9856
161.5355-0.2152-0.41152.5849-0.9541-0.0328-0.1280.1624-0.2771-0.53480.2896-0.31790.11770.0515-0.16160.1065-0.22720.0950.0388-0.0662-0.1105-1.70512.6134.2013
172.23130.79350.59461.84510.74090.2668-0.16750.0391-0.0678-0.04590.3755-0.48390.20410.3486-0.208-0.1897-0.01920.06280.0657-0.12120.021112.270714.547949.9885
181.7669-0.122-0.03671.99310.44621.4993-0.0739-0.02680.1484-0.2750.11650.1156-0.13470.0155-0.0426-0.0509-0.16190.0278-0.03020.0224-0.1957-11.959324.417339.5607
193.5071.8302-3.57291.00041.347813.5385-0.1151-0.03820.3764-0.1507-0.18310.1872-0.2498-0.25140.29820.0648-0.08660.0203-0.037-0.0591-0.0598-17.88766.723945.989
202.3411.4744-0.18152.2089-0.24541.7442-0.15180.1960.0465-0.15020.27420.13260.18140.0111-0.12240.0031-0.07090.0392-0.0263-0.0074-0.0965-12.13235.089740.9373
211.187-0.35630.39512.85861.19812.57510.1426-0.2594-0.24410.5852-0.45830.38440.7643-0.98750.31560.0146-0.36820.0669-0.0291-0.0839-0.3765-19.68624.8067-49.1705
225.6807-3.16113.13372.96592.28839.688-0.0561-0.6615-0.01360.7498-0.0613-0.44020.1951-0.50740.11740.2726-0.1333-0.1437-0.2650.0025-0.2716-4.80796.2309-33.8497
233.7593-0.22230.78261.2481.48575.8313-0.0281-0.60160.38150.66080.1282-0.4050.42770.4081-0.10.128-0.0081-0.3058-0.3318-0.0949-0.01987.82155.7738-35.1129
243.2652-4.444-2.22734.487-0.69174.3075-0.02050.0093-0.27070.21870.01740.2730.58410.09280.0031-0.0360.1019-0.0832-0.1103-0.0440.08078.212613.5934-54.77
251.44470.23741.24122.14870.04671.74510.11470.1909-0.42870.62630.0419-0.74350.61250.2105-0.15660.07970.0695-0.1254-0.3277-0.0314-0.02043.46030.8678-54.6505
261.05920.41650.10714.74620.98252.50110.09190.1152-0.0472-0.0669-0.1167-0.29810.1335-0.01830.0248-0.1999-0.009-0.0067-0.04360.0922-0.12716.5841-33.26079.3161
271.13280.22730.28670.81780.6242.7119-0.0365-0.21760.06120.1250.02450.1242-0.0215-0.17080.012-0.0855-0.00390.0389-0.0139-0.0041-0.118714.4333-33.433222.4162
282.7974-0.39740.51772.70450.73284.84880.0889-0.0312-0.35270.17630.2037-0.46810.58790.5164-0.2926-0.05990.0162-0.0448-0.0775-0.0062-0.033330.5803-51.12539.1196
29-0.0132-0.0914-1.08143.3411-1.07722.45610.03910.2226-0.0524-0.1295-0.1087-0.1150.40740.35480.0696-0.06130.02950.01080.1154-0.0676-0.110233.5789-47.8124-5.7236
300.709-0.2132-0.22070.0223-0.68421.509-0.03670.20050.0595-0.20180.0029-0.0420.1507-0.00130.0339-0.0097-0.050.0098-0.02590.0156-0.118619.0986-39.2965-3.6198
312.14680.37930.42482.09370.30051.6284-0.03470.3610.1548-0.2510.194-0.1111-0.27090.3674-0.1593-0.0744-0.15860.01180.03720.0765-0.240735.1117-13.1474-15.2352
322.5317-1.3596-0.1244.56321.33782.4971-0.04160.29390.5841-0.03680.1677-0.5621-0.50390.5091-0.1261-0.0511-0.18390.0057-0.1560.0946-0.120135.09760.7851-8.9412
334.80550.5746-1.21033.97032.51581.8709-0.1938-0.66121.04450.60320.3012-0.0319-0.34250.3824-0.10740.0630.0263-0.1284-0.1997-0.1103-0.046128.65638.84757.632
345.3546-2.029-4.72683.5289-6.40683.8519-0.1080.50640.29010.2088-0.05920.4078-0.17420.19680.16720.02580.02-0.02680.013-0.0698-0.026219.038-10.993812.494
35-0.13440.87890.14633.1083-1.24270.1564-0.00890.03080.58530.15950.00490.4609-0.4273-0.2780.004-0.00410.0495-0.0054-0.10350.08770.012715.9439-4.8189-0.8573
362.30450.33520.62962.27380.1721.97850.1558-0.222-0.20910.3323-0.06370.16990.5968-0.3382-0.0921-0.0215-0.23750.1117-0.043-0.0277-0.3571-1.0041-53.7305-19.0695
372.3696-1.24630.79813.00220.12492.27730.2896-0.0352-0.39960.3273-0.13290.07131.0351-0.1893-0.15660.1443-0.24870.0345-0.0311-0.0277-0.2616-1.6759-61.3987-22.9124
386.277-0.38611.58596.4707-0.58482.42140.53360.8301-0.8349-0.7347-0.33980.6660.9751-0.7649-0.1938-0.1854-0.2462-0.06110.2057-0.2692-0.3292-20.9453-56.6285-36.9098
396.24440.35190.15470.8852-0.1381.060.04610.1211-0.0252-0.2553-0.04510.67450.5577-0.9404-0.001-0.1993-0.1282-0.0140.3452-0.1056-0.0818-19.5864-42.7388-44.3422
403.63260.1998-0.6984-0.21410.65162.40.16780.00640.16830.0267-0.28130.50640.002-0.73510.1135-0.3146-0.07460.10890.1542-0.1191-0.1182-12.4584-38.1125-31.2355
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1CHAIN A AND RESSEQ 4:29
2X-RAY DIFFRACTION2CHAIN A AND RESSEQ 30:147
3X-RAY DIFFRACTION3CHAIN A AND RESSEQ 148:223
4X-RAY DIFFRACTION4CHAIN A AND RESSEQ 224:337
5X-RAY DIFFRACTION5CHAIN A AND RESSEQ 338:411
6X-RAY DIFFRACTION6CHAIN B AND RESSEQ 3:51
7X-RAY DIFFRACTION7CHAIN B AND RESSEQ 52:164
8X-RAY DIFFRACTION8CHAIN B AND RESSEQ 165:315
9X-RAY DIFFRACTION9CHAIN B AND RESSEQ 316:361
10X-RAY DIFFRACTION10CHAIN B AND RESSEQ 362:411
11X-RAY DIFFRACTION11CHAIN C AND RESSEQ 4:52
12X-RAY DIFFRACTION12CHAIN C AND RESSEQ 53:157
13X-RAY DIFFRACTION13CHAIN C AND RESSEQ 158:244
14X-RAY DIFFRACTION14CHAIN C AND RESSEQ 245:340
15X-RAY DIFFRACTION15CHAIN C AND RESSEQ 341:411
16X-RAY DIFFRACTION16CHAIN D AND RESSEQ 3:29
17X-RAY DIFFRACTION17CHAIN D AND RESSEQ 30:162
18X-RAY DIFFRACTION18CHAIN D AND RESSEQ 163:336
19X-RAY DIFFRACTION19CHAIN D AND RESSEQ 337:365
20X-RAY DIFFRACTION20CHAIN D AND RESSEQ 366:411
21X-RAY DIFFRACTION21CHAIN E AND RESSEQ 6:208
22X-RAY DIFFRACTION22CHAIN E AND RESSEQ 209:248
23X-RAY DIFFRACTION23CHAIN E AND RESSEQ 249:337
24X-RAY DIFFRACTION24CHAIN E AND RESSEQ 338:356
25X-RAY DIFFRACTION25CHAIN E AND RESSEQ 357:411
26X-RAY DIFFRACTION26CHAIN F AND RESSEQ 4:52
27X-RAY DIFFRACTION27CHAIN F AND RESSEQ 53:208
28X-RAY DIFFRACTION28CHAIN F AND RESSEQ 209:311
29X-RAY DIFFRACTION29CHAIN F AND RESSEQ 312:350
30X-RAY DIFFRACTION30CHAIN F AND RESSEQ 351:411
31X-RAY DIFFRACTION31CHAIN G AND RESSEQ 4:157
32X-RAY DIFFRACTION32CHAIN G AND RESSEQ 158:229
33X-RAY DIFFRACTION33CHAIN G AND RESSEQ 230:336
34X-RAY DIFFRACTION34CHAIN G AND RESSEQ 337:352
35X-RAY DIFFRACTION35CHAIN G AND RESSEQ 353:411
36X-RAY DIFFRACTION36CHAIN H AND RESSEQ 4:134
37X-RAY DIFFRACTION37CHAIN H AND RESSEQ 135:206
38X-RAY DIFFRACTION38CHAIN H AND RESSEQ 207:312
39X-RAY DIFFRACTION39CHAIN H AND RESSEQ 313:356
40X-RAY DIFFRACTION40CHAIN H AND RESSEQ 357:411

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