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Open data
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Basic information
Entry | Database: PDB / ID: 4bhu | ||||||
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Title | Crystal structure of BslA - A bacterial hydrophobin | ||||||
![]() | (UNCHARACTERIZED PROTEIN YUAB) x 2 | ||||||
![]() | STRUCTURAL PROTEIN / BIOFILM | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Rao, F.V. / Hobley, L. / Ostrowski, A. / Bromley, K.M. / Porter, M. / Prescott, A.R. / Swedlow, J.R. / MacPhee, C.E. / van Aalten, D.M.F. / Stanley-Wall, N.R. | ||||||
![]() | ![]() Title: Bsla is a Self-Assembling Bacterial Hydrophobin that Coats the Bacillus Subtilis Biofilm. Authors: Hobley, L. / Ostrowski, A. / Rao, F.V. / Bromley, K.M. / Porter, M. / Prescott, A.R. / Macphee, C.E. / Van Aalten, D.M.F. / Stanley-Wall, N.R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 491.5 KB | Display | ![]() |
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PDB format | ![]() | 431.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 513.7 KB | Display | ![]() |
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Full document | ![]() | 524.6 KB | Display | |
Data in XML | ![]() | 64.1 KB | Display | |
Data in CIF | ![]() | 91.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 14112.729 Da / Num. of mol.: 9 / Fragment: RESIDUES 48-172 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: NCIB 3610 / Production host: ![]() ![]() #2: Protein | | Mass: 14113.713 Da / Num. of mol.: 1 / Fragment: RESIDUES 48-172 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: NCIB 3610 / Production host: ![]() ![]() #3: Chemical | ChemComp-GOL / #4: Chemical | ChemComp-CL / #5: Water | ChemComp-HOH / | Sequence details | THE FIRST 5 AMINO ACIDS (GPLGS) ARE FROM THE EXPRESSION | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.34 Å3/Da / Density % sol: 63 % / Description: NONE |
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Crystal grow | Details: 1.9 M LI2SO4, 0.1 M HEPES, PH 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Dec 15, 2012 / Details: KIRKPATRICK BAEZ BIMORPH MIRROR PAIR |
Radiation | Monochromator: SI(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9797 Å / Relative weight: 1 |
Reflection | Resolution: 1.91→77.19 Å / Num. obs: 135912 / % possible obs: 100 % / Observed criterion σ(I): 2 / Redundancy: 13.3 % / Biso Wilson estimate: 24.68 Å2 / Rmerge(I) obs: 0.09 / Net I/σ(I): 18.04 |
Reflection shell | Resolution: 1.91→1.98 Å / Redundancy: 13.5 % / Rmerge(I) obs: 0.67 / Mean I/σ(I) obs: 4.27 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: NONE Resolution: 1.91→77.195 Å / SU ML: 0.16 / σ(F): 1.34 / Phase error: 18.52 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 28.7 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.91→77.195 Å
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Refine LS restraints |
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LS refinement shell |
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