Mass: 18.015 Da / Num. of mol.: 391 / Source method: isolated from a natural source / Formula: H2O
Sequence details
THE CRYSTALLIZED PROTEIN CONTAINED RESIDUES 25-169 OF THE WILD-TYPE SEQUENCE. A FURTHER TWO ...THE CRYSTALLIZED PROTEIN CONTAINED RESIDUES 25-169 OF THE WILD-TYPE SEQUENCE. A FURTHER TWO RESIDUES ( GLY-PRO) WERE APPENDED AT THE N-TERMINUS RESULTING FROM THE CLEAVED HIS-TAG, BUT THESE WERE NOT VISIBLE IN THE ELECTRON DENSITY. RESIDUE NUMBERING WAS RELATIVE TO THE FULL-LENGTH WILD-TYPE SEQUENCE.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.3 Å3/Da / Density % sol: 46.5 % Description: DIFFRACTION DATA WERE RECORDED IN TWO PASSES, AT 2.32 A AND 1.25 A RESOLUTIONS, RESPECTIVELY.
Crystal grow
Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: HANGING DROP VAPOUR DIFFUSION AT 291 K. DROPS CONSISTED OF 1 MICROLITRE OF PROTEIN AT 10 MG/ML IN 50 MM HEPES PH 7.5, 500 MM NACL PLUS 1 MICROLITRE OF PRECIPITANT SOLUTION COMPRISED OF 0.2 M ...Details: HANGING DROP VAPOUR DIFFUSION AT 291 K. DROPS CONSISTED OF 1 MICROLITRE OF PROTEIN AT 10 MG/ML IN 50 MM HEPES PH 7.5, 500 MM NACL PLUS 1 MICROLITRE OF PRECIPITANT SOLUTION COMPRISED OF 0.2 M POTASSIUM NITRATE, 25% PEG 3350, 6% MPD, 15% GLYCEROL, 1 MM SPERMIDINE