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Yorodumi- PDB-4asw: Structure of the complex between the N-terminal dimerisation doma... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4asw | |||||||||
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Title | Structure of the complex between the N-terminal dimerisation domain of Sgt2 and the UBL domain of Get5 | |||||||||
Components |
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Keywords | CHAPERONE / TAIL-ANCHORED / POST-TRANSLATIONAL TARGETING | |||||||||
Function / homology | Function and homology information cell morphogenesis involved in conjugation with cellular fusion / TRC complex / Insertion of tail-anchored proteins into the endoplasmic reticulum membrane / protein insertion into ER membrane / post-translational protein targeting to endoplasmic reticulum membrane / cytoplasmic stress granule / response to heat / molecular adaptor activity / membrane / identical protein binding ...cell morphogenesis involved in conjugation with cellular fusion / TRC complex / Insertion of tail-anchored proteins into the endoplasmic reticulum membrane / protein insertion into ER membrane / post-translational protein targeting to endoplasmic reticulum membrane / cytoplasmic stress granule / response to heat / molecular adaptor activity / membrane / identical protein binding / nucleus / cytosol / cytoplasm Similarity search - Function | |||||||||
Biological species | SACCHAROMYCES CEREVISIAE (brewer's yeast) | |||||||||
Method | SOLUTION NMR / ARIA | |||||||||
Authors | Simon, A.C. / Simpson, P.J. / Goldstone, R.M. / Krysztofinska, E.M. / Murray, J.W. / High, S. / Isaacson, R.L. | |||||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2013 Title: Structure of the Sgt2/Get5 Complex Provides Insights Into Get-Mediated Targeting of Tail-Anchored Membrane Proteins Authors: Simon, A.C. / Simpson, P.J. / Goldstone, R.M. / Krysztofinska, E.M. / Murray, J.W. / High, S. / Isaacson, R.L. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4asw.cif.gz | 448.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4asw.ent.gz | 379.4 KB | Display | PDB format |
PDBx/mmJSON format | 4asw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/as/4asw ftp://data.pdbj.org/pub/pdb/validation_reports/as/4asw | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10098.163 Da / Num. of mol.: 2 / Fragment: DIMERISATION DOMAIN, RESIDUES 1-78 Source method: isolated from a genetically manipulated source Source: (gene. exp.) SACCHAROMYCES CEREVISIAE (brewer's yeast) Plasmid: PET46-SGT2_NT / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): ROSETTA / References: UniProt: Q12118 #2: Protein | | Mass: 9265.789 Da / Num. of mol.: 1 / Fragment: RESIDUES 70-152 Source method: isolated from a genetically manipulated source Source: (gene. exp.) SACCHAROMYCES CEREVISIAE (brewer's yeast) Plasmid: PET46-SGT2_NT / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): ROSETTA / References: UniProt: Q12285 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: NONE |
-Sample preparation
Details | Contents: 90% WATER/10% D2O |
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Sample conditions | Ionic strength: 250 mM / pH: 6.0 / Pressure: 1 atm / Temperature: 303.0 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: ARIA / Software ordinal: 1 / Details: BMRB 18342 | |||||||||
NMR representative | Selection criteria: lowest energy | |||||||||
NMR ensemble | Conformer selection criteria: LOWEST ENERGY / Conformers calculated total number: 100 / Conformers submitted total number: 9 |