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- PDB-4asw: Structure of the complex between the N-terminal dimerisation doma... -
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Basic information
Entry | Database: PDB / ID: 4asw | |||||||||
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Title | Structure of the complex between the N-terminal dimerisation domain of Sgt2 and the UBL domain of Get5 | |||||||||
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![]() | CHAPERONE / TAIL-ANCHORED / POST-TRANSLATIONAL TARGETING | |||||||||
Function / homology | ![]() cell morphogenesis involved in conjugation with cellular fusion / Insertion of tail-anchored proteins into the endoplasmic reticulum membrane / TRC complex / tail-anchored membrane protein insertion into ER membrane / protein insertion into ER membrane / post-translational protein targeting to endoplasmic reticulum membrane / cytoplasmic stress granule / protein-folding chaperone binding / response to heat / molecular adaptor activity ...cell morphogenesis involved in conjugation with cellular fusion / Insertion of tail-anchored proteins into the endoplasmic reticulum membrane / TRC complex / tail-anchored membrane protein insertion into ER membrane / protein insertion into ER membrane / post-translational protein targeting to endoplasmic reticulum membrane / cytoplasmic stress granule / protein-folding chaperone binding / response to heat / molecular adaptor activity / identical protein binding / membrane / nucleus / cytoplasm / cytosol Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | SOLUTION NMR / ARIA | |||||||||
![]() | Simon, A.C. / Simpson, P.J. / Goldstone, R.M. / Krysztofinska, E.M. / Murray, J.W. / High, S. / Isaacson, R.L. | |||||||||
![]() | ![]() Title: Structure of the Sgt2/Get5 Complex Provides Insights Into Get-Mediated Targeting of Tail-Anchored Membrane Proteins Authors: Simon, A.C. / Simpson, P.J. / Goldstone, R.M. / Krysztofinska, E.M. / Murray, J.W. / High, S. / Isaacson, R.L. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 448.9 KB | Display | ![]() |
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PDB format | ![]() | 379.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 282.6 KB | Display | ![]() |
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Full document | ![]() | 420.3 KB | Display | |
Data in XML | ![]() | 41.6 KB | Display | |
Data in CIF | ![]() | 56.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 10098.163 Da / Num. of mol.: 2 / Fragment: DIMERISATION DOMAIN, RESIDUES 1-78 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Plasmid: PET46-SGT2_NT / Production host: ![]() ![]() #2: Protein | | Mass: 9265.789 Da / Num. of mol.: 1 / Fragment: RESIDUES 70-152 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Plasmid: PET46-SGT2_NT / Production host: ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: NONE |
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Sample preparation
Details | Contents: 90% WATER/10% D2O |
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Sample conditions | Ionic strength: 250 mM / pH: 6.0 / Pressure: 1 atm / Temperature: 303.0 K |
-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: ARIA / Software ordinal: 1 / Details: BMRB 18342 | |||||||||
NMR representative | Selection criteria: lowest energy | |||||||||
NMR ensemble | Conformer selection criteria: LOWEST ENERGY / Conformers calculated total number: 100 / Conformers submitted total number: 9 |