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- PDB-4arx: Lepidoptera-specific toxin Cry1Ac from Bacillus thuringiensis ssp... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4arx | ||||||
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Title | Lepidoptera-specific toxin Cry1Ac from Bacillus thuringiensis ssp. kurstaki HD-73 | ||||||
![]() | PESTICIDAL CRYSTAL PROTEIN CRY1AC | ||||||
![]() | TOXIN / MEMBRANE PORE-FORMING TOXIN / INSECTICIDAL PROTEIN LEPIDOPTERAN SPECIFICITY / RECEPTOR BINDING SITE | ||||||
Function / homology | ![]() symbiont-mediated killing of host cell / sporulation resulting in formation of a cellular spore / toxin activity / signaling receptor binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Derbyshire, D.J. / Carroll, J. / Ellar, D.J. / Li, J. | ||||||
![]() | ![]() Title: Structural Basis of Galnac-Dependent Receptor Recognition by B. Thuringiensis Toxin Cry1Ac Authors: Derbyshire, D.J. / Carroll, J. / Ellar, D.J. / Li, J. #1: Journal: Acta Crystallogr.,Sect.D / Year: 2001 Title: Crystallization of the Bacillus Thuringiensis Toxin Cry1Ac and its Complex with the Receptor Ligand N-Aceryl-D-Galactosamine Authors: Derbyshire, D.J. / Ellar, D.J. / Li, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 460.7 KB | Display | ![]() |
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PDB format | ![]() | 378.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 467.9 KB | Display | ![]() |
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Full document | ![]() | 480 KB | Display | |
Data in XML | ![]() | 81.3 KB | Display | |
Data in CIF | ![]() | 115.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4aryC ![]() 1ciyS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: _ / End auth comp-ID: PRO / End label comp-ID: PRO / Refine code: _
NCS ensembles :
NCS oper:
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Components
#1: Protein | Mass: 64993.480 Da / Num. of mol.: 4 Fragment: ACTIVATED TOXIN OBTAINED BY TRYPSIN CLEAVAGE, RESIDUES 31-609 Source method: isolated from a natural source Source: (natural) ![]() ![]() Variant: HD-73 / References: UniProt: P05068 #2: Chemical | ChemComp-13D / #3: Chemical | ChemComp-GOL / | #4: Water | ChemComp-HOH / | Nonpolymer details | 1,3-DIAMINOPROPANE (13D): USING 1,3-DIAMINOPROPANE AS BUFFER DURING PURIFICATION PREVENTED ...1,3-DIAMINOPRO | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 48 % Description: FROZEN CRYSTALS WERE ANNEALED BY THAWING IN THE CRYO -PROTECTANT SOLUTION AND RE-FROZEN IN THE VAPOUR OF LIQUID N2. |
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Crystal grow | Method: microdialysis / pH: 9.8 Details: MICRODIALYSIS AGAINST 65-69 MM PIPERAZINE PH 9.8, 25-29 MM NABR, 21-25% GLYCEROL, 9% MPD, 1% PEG350MME. FOR DETAILS SEE REFERNCE 1 IN REMARK 1 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Sep 19, 1997 / Details: MONOCHROMATOR |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.4 Å / Relative weight: 1 |
Reflection | Resolution: 2.34→43.19 Å / Num. obs: 95755 / % possible obs: 90.4 % / Observed criterion σ(I): 0 / Redundancy: 2.4 % / Biso Wilson estimate: 29.267 Å2 / Rmerge(I) obs: 0.1 / Net I/σ(I): 11.2 |
Reflection shell | Resolution: 2.34→2.47 Å / Redundancy: 1.9 % / Rmerge(I) obs: 0.22 / Mean I/σ(I) obs: 3.7 / % possible all: 75.1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1CIY Resolution: 2.35→112.51 Å / Cor.coef. Fo:Fc: 0.938 / Cor.coef. Fo:Fc free: 0.912 / SU B: 6.302 / SU ML: 0.151 / Cross valid method: THROUGHOUT / ESU R Free: 0.246 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. LOCAL NCS RESTRAINTS WERE DEFINED AUTOMATICALLY. JELLY BODY RESTRAINTS AGAINST EXCESSIVE POSITION SHIFTS WERE APPLIED.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 21.553 Å2
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Refinement step | Cycle: LAST / Resolution: 2.35→112.51 Å
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Refine LS restraints |
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