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Open data
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Basic information
Entry | Database: PDB / ID: 1ciy | ||||||
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Title | INSECTICIDAL TOXIN: STRUCTURE AND CHANNEL FORMATION | ||||||
![]() | CRYIA(A) | ||||||
![]() | TOXIN / DELTA-ENDOTOXIN CRYIA(A) / ICP | ||||||
Function / homology | ![]() sporulation resulting in formation of a cellular spore / : / toxin activity / signaling receptor binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Grochulski, P. / Cygler, M. | ||||||
![]() | ![]() Title: Bacillus thuringiensis CryIA(a) insecticidal toxin: crystal structure and channel formation. Authors: Grochulski, P. / Masson, L. / Borisova, S. / Pusztai-Carey, M. / Schwartz, J.L. / Brousseau, R. / Cygler, M. #1: ![]() Title: Crystallization and Preliminary X-Ray Diffraction Studies of the Lepidopteran-Specific Insecticidal Crystal Protein Cryla(A) Authors: Borisova, S. / Grochulski, P. / Van Faassen, H. / Pusztai-Carey, M. / Masson, L. / Cygler, M. #2: ![]() Title: Crystal Structure of Insecticidal Delta-Endotoxin from Bacillus Thuringiensis at 2.5 A Resolution Authors: Li, J.D. / Carroll, J. / Ellar, D.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 127.4 KB | Display | ![]() |
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PDB format | ![]() | 98.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 414.8 KB | Display | ![]() |
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Full document | ![]() | 424.7 KB | Display | |
Data in XML | ![]() | 22.8 KB | Display | |
Data in CIF | ![]() | 32.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 66166.930 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.53 Å3/Da / Density % sol: 55 % | ||||||||||||||||||||||||||||||
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Crystal | *PLUS | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 10 / Method: vapor diffusion / Details: Borisova, S., (1994) J.Mol.Biol., 243, 530. | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: RIGAKU RAXIS II / Detector: IMAGE PLATE |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.25→154 Å / Num. obs: 33090 / % possible obs: 74 % / Observed criterion σ(I): 1 / Redundancy: 2.6 % / Rmerge(I) obs: 0.059 |
Reflection | *PLUS Num. measured all: 86937 |
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Processing
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Refinement | Resolution: 2.25→8 Å / σ(F): 4
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Displacement parameters | Biso mean: 27.5 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.25 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.25→8 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS σ(F): 2 / Rfactor all: 0.21 / Rfactor obs: 0.168 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 23.6 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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