+Open data
-Basic information
Entry | Database: PDB / ID: 1ciy | ||||||
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Title | INSECTICIDAL TOXIN: STRUCTURE AND CHANNEL FORMATION | ||||||
Components | CRYIA(A) | ||||||
Keywords | TOXIN / DELTA-ENDOTOXIN CRYIA(A) / ICP | ||||||
Function / homology | Function and homology information sporulation resulting in formation of a cellular spore / : / toxin activity / signaling receptor binding Similarity search - Function | ||||||
Biological species | Bacillus thuringiensis (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.25 Å | ||||||
Authors | Grochulski, P. / Cygler, M. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1995 Title: Bacillus thuringiensis CryIA(a) insecticidal toxin: crystal structure and channel formation. Authors: Grochulski, P. / Masson, L. / Borisova, S. / Pusztai-Carey, M. / Schwartz, J.L. / Brousseau, R. / Cygler, M. #1: Journal: J.Mol.Biol. / Year: 1994 Title: Crystallization and Preliminary X-Ray Diffraction Studies of the Lepidopteran-Specific Insecticidal Crystal Protein Cryla(A) Authors: Borisova, S. / Grochulski, P. / Van Faassen, H. / Pusztai-Carey, M. / Masson, L. / Cygler, M. #2: Journal: Nature / Year: 1991 Title: Crystal Structure of Insecticidal Delta-Endotoxin from Bacillus Thuringiensis at 2.5 A Resolution Authors: Li, J.D. / Carroll, J. / Ellar, D.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ciy.cif.gz | 127.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ciy.ent.gz | 98.4 KB | Display | PDB format |
PDBx/mmJSON format | 1ciy.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ci/1ciy ftp://data.pdbj.org/pub/pdb/validation_reports/ci/1ciy | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 66166.930 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus thuringiensis (bacteria) / Strain: HD-1 / Variant: KURSTAKI / Plasmid: PUC8 PROTEIN PURIFIED FROM / Production host: Escherichia coli (E. coli) / References: UniProt: P0A366 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 3.53 Å3/Da / Density % sol: 55 % | ||||||||||||||||||||||||||||||
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Crystal | *PLUS | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 10 / Method: vapor diffusion / Details: Borisova, S., (1994) J.Mol.Biol., 243, 530. | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: RIGAKU RAXIS II / Detector: IMAGE PLATE |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.25→154 Å / Num. obs: 33090 / % possible obs: 74 % / Observed criterion σ(I): 1 / Redundancy: 2.6 % / Rmerge(I) obs: 0.059 |
Reflection | *PLUS Num. measured all: 86937 |
-Processing
Software |
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Refinement | Resolution: 2.25→8 Å / σ(F): 4
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Displacement parameters | Biso mean: 27.5 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.25 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.25→8 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Version: 3.1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS σ(F): 2 / Rfactor all: 0.21 / Rfactor obs: 0.168 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 23.6 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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