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Yorodumi- PDB-4abt: Crystal structure of Type IIF restriction endonuclease NgoMIV wit... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4abt | ||||||
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Title | Crystal structure of Type IIF restriction endonuclease NgoMIV with cognate uncleaved DNA | ||||||
Components |
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Keywords | HYDROLASE/DNA / HYDROLASE-DNA COMPLEX | ||||||
Function / homology | Function and homology information type II site-specific deoxyribonuclease / type II site-specific deoxyribonuclease activity / DNA restriction-modification system / metal ion binding Similarity search - Function | ||||||
Biological species | NEISSERIA GONORRHOEAE (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.22 Å | ||||||
Authors | Manakova, E.N. / Grazulis, S. / Zaremba, M. / Tamulaitiene, G. / Golovenko, D. / Siksnys, V. | ||||||
Citation | Journal: To be Published Title: Structure of Type Iif Restriction Endonuclease Ngomiv with Cognate Uncleaved DNA Authors: Manakova, E.N. / Grazulis, S. / Zaremba, M. / Tamulaitiene, G. / Golovenko, D. / Siksnys, V. #1: Journal: Nucleic Acids Res. / Year: 2012 Title: Structural Mechanisms of the Degenerate Sequence Recognition by Bse634I Restriction Endonuclease. Authors: Manakova, E. / Grazulis, S. / Zaremba, M. / Tamulaitiene, G. / Golovenko, D. / Siksnys, V. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4abt.cif.gz | 144.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4abt.ent.gz | 108.7 KB | Display | PDB format |
PDBx/mmJSON format | 4abt.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ab/4abt ftp://data.pdbj.org/pub/pdb/validation_reports/ab/4abt | HTTPS FTP |
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-Related structure data
Related structure data | 1fiuS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 31810.902 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) NEISSERIA GONORRHOEAE (bacteria) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): HMS 174 DE3 References: UniProt: P31032, type II site-specific deoxyribonuclease #2: DNA chain | Mass: 3351.173 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) NEISSERIA GONORRHOEAE (bacteria) #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2 Å3/Da / Density % sol: 39.1 % / Description: NONE |
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Crystal grow | pH: 7.5 / Details: NAHEPES 0.1M, CACL2 0.2M, MPD 24%, PH 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: EMBL/DESY, HAMBURG / Beamline: X11 / Wavelength: 0.812 |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Nov 3, 2002 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.812 Å / Relative weight: 1 |
Reflection | Resolution: 2.22→11.95 Å / Num. obs: 28869 / % possible obs: 98.2 % / Observed criterion σ(I): 0 / Redundancy: 4 % / Rmerge(I) obs: 0.08 / Net I/σ(I): 8 |
Reflection shell | Resolution: 2.22→2.27 Å / Redundancy: 2.4 % / Rmerge(I) obs: 0.34 / Mean I/σ(I) obs: 1.8 / % possible all: 84.1 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1FIU Resolution: 2.22→11.95 Å / Cor.coef. Fo:Fc: 0.946 / Cor.coef. Fo:Fc free: 0.915 / Cross valid method: THROUGHOUT / ESU R: 0.373 / ESU R Free: 0.236 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 19.898 Å2
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Refinement step | Cycle: LAST / Resolution: 2.22→11.95 Å
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Refine LS restraints |
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