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Open data
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Basic information
Entry | Database: PDB / ID: 4aa9 | ||||||
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Title | Camel chymosin at 1.6A resolution | ||||||
![]() | CHYMOSIN | ||||||
![]() | HYDROLASE / ASPARTIC PEPTIDASE / RENNET | ||||||
Function / homology | ![]() chymosin / digestion / proteolysis involved in protein catabolic process / aspartic-type endopeptidase activity Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Langholm Jensen, J. / Molgaard, A. / Navarro Poulsen, J.C. / van den Brink, J.M. / Harboe, M. / Simonsen, J.B. / Qvist, K.B. / Larsen, S. | ||||||
![]() | ![]() Title: Camel and Bovine Chymosin: The Relationship between Their Structures and Cheese-Making Properties. Authors: Langholm Jensen, J. / Molgaard, A. / Navarro Poulsen, J.C. / Harboe, M.K. / Simonsen, J.B. / Lorentzen, A.M. / Hjerno, K. / van den Brink, J.M. / Qvist, K.B. / Larsen, S. | ||||||
History |
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Remark 700 | SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS BELOW ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 6-STRANDED BARREL THIS IS REPRESENTED BY A 7-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 89.2 KB | Display | ![]() |
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PDB format | ![]() | 65.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4aa8C ![]() 1cmsS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 35370.461 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||||
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#2: Sugar | ChemComp-NAG / | ||||
#3: Chemical | ChemComp-SO4 / #4: Chemical | ChemComp-GOL / | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3 Å3/Da / Density % sol: 58 % / Description: NONE |
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Crystal grow | pH: 5.5 Details: RESERVOIR: 2 M (NH4)2SO4, 100 MM BIS-TRIS, PH 5.5. SAMPLE: 30 MG/ML PROTEIN IN 50 MM BIS-TRIS, PH 6.0. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH MAR165 / Detector: CCD / Date: Dec 3, 2011 Details: MULTILAYER MIRROR, CURVED TO FOCUS IN THE VERTICAL (R 400 M). |
Radiation | Monochromator: BENT SI (111) CRYSTAL, HORIZONTALLY FOCUSING. Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.04 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→30 Å / Num. obs: 62765 / % possible obs: 99 % / Observed criterion σ(I): 15 / Redundancy: 8.6 % / Biso Wilson estimate: 19.66 Å2 / Rmerge(I) obs: 0.04 / Net I/σ(I): 31 |
Reflection shell | Resolution: 1.6→1.7 Å / Redundancy: 9.6 % / Rmerge(I) obs: 0.14 / Mean I/σ(I) obs: 6 / % possible all: 23 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1CMS Resolution: 1.6→24.795 Å / SU ML: 0.2 / σ(F): 2 / Phase error: 18.7 / Stereochemistry target values: ML Details: DISORDERED LOOP 159-162 WAS MODELLED STEREOCHEMICALLY. VM CALCULATED USING A TOTAL MASS OF 40 KDA (DETERMINED USING MASS SPECTROMETRY) DUE TO TWO GLYCOSYLATIONS).
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Solvent computation | Shrinkage radii: 0.95 Å / VDW probe radii: 1.2 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 47.012 Å2 / ksol: 0.424 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 1.6→24.795 Å
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Refine LS restraints |
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LS refinement shell |
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