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- PDB-3zo7: Crystal structure of ClcFE27A with substrate -

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Open data


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Basic information

Entry
Database: PDB / ID: 3zo7
TitleCrystal structure of ClcFE27A with substrate
Components5-CHLOROMUCONOLACTONE DEHALOGENASE
KeywordsLYASE / FERREDOXIN FOLD / CHLOROCATECHOL PATHWAY / DEHALOGENASE
Function / homology
Function and homology information


muconolactone Delta-isomerase / muconolactone delta-isomerase activity / beta-ketoadipate pathway
Similarity search - Function
Muconolactone delta-isomerase / Muconolactone isomerase domain / Muconolactone delta-isomerase / Dimeric alpha+beta barrel / Dimeric alpha-beta barrel / Alpha-Beta Plaits / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
Chem-K6H / Muconolactone Delta-isomerase
Similarity search - Component
Biological speciesRHODOCOCCUS OPACUS (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.224 Å
AuthorsRoth, C. / Groening, J.A.D. / Kaschabek, S.R. / Schloemann, M. / Straeter, N.
CitationJournal: Mol.Microbiol. / Year: 2013
Title: Crystal Structure and Catalytic Mechanism of Chloromuconolactone Dehalogenase Clcf from Rhodococcus Opacus 1Cp.
Authors: Roth, C. / Janosch, A.D. / Kaschabek, S.R. / Schloemann, M. / Straeter, N.
History
DepositionFeb 20, 2013Deposition site: PDBE / Processing site: PDBE
Revision 1.0Mar 6, 2013Provider: repository / Type: Initial release
Revision 1.1Mar 13, 2013Group: Database references
Revision 1.2Mar 20, 2013Group: Atomic model / Other
Revision 1.3Apr 24, 2013Group: Database references
Revision 1.4May 8, 2024Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Other
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_database_status / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.status_code_sf / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id
Remark 700 SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS BELOW ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 8-STRANDED BARREL THIS IS REPRESENTED BY A 9-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEETS PRESENTED AS "BA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 8-STRANDED BARREL THIS IS REPRESENTED BY A 9-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEETS PRESENTED AS "EA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 8-STRANDED BARREL THIS IS REPRESENTED BY A 9-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEETS PRESENTED AS "FA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 8-STRANDED BARREL THIS IS REPRESENTED BY A 9-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEETS PRESENTED AS "HA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 8-STRANDED BARREL THIS IS REPRESENTED BY A 9-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL.

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: 5-CHLOROMUCONOLACTONE DEHALOGENASE
B: 5-CHLOROMUCONOLACTONE DEHALOGENASE
C: 5-CHLOROMUCONOLACTONE DEHALOGENASE
D: 5-CHLOROMUCONOLACTONE DEHALOGENASE
E: 5-CHLOROMUCONOLACTONE DEHALOGENASE
F: 5-CHLOROMUCONOLACTONE DEHALOGENASE
G: 5-CHLOROMUCONOLACTONE DEHALOGENASE
H: 5-CHLOROMUCONOLACTONE DEHALOGENASE
I: 5-CHLOROMUCONOLACTONE DEHALOGENASE
J: 5-CHLOROMUCONOLACTONE DEHALOGENASE
hetero molecules


Theoretical massNumber of molelcules
Total (without water)113,78934
Polymers111,52710
Non-polymers2,26224
Water8,899494
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area32500 Å2
ΔGint-275.5 kcal/mol
Surface area39500 Å2
MethodPISA
Unit cell
Length a, b, c (Å)77.836, 103.112, 137.767
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121
Noncrystallographic symmetry (NCS)NCS oper:
IDCodeMatrixVector
1given(-0.3134, -0.7734, 0.5511), (-0.7429, -0.1617, -0.6495), (0.5914, -0.613, -0.5239)105.2033, 112.6528, 22.9989
2given(0.3393, -0.8339, 0.4353), (0.7314, 0.5249, 0.4355), (-0.5916, 0.1706, 0.788)73.8097, -22.3116, 28.1426
3given(0.7938, -0.3875, 0.4688), (-0.398, -0.9138, -0.0814), (0.4599, -0.1219, -0.8796)24.7598, 124.5439, 9.9755
4given(-0.7863, -0.5877, 0.1904), (0.4095, -0.265, 0.873), (-0.4626, 0.7644, 0.4491)127.6388, 30.1797, -6.3423
5given(-0.7885, 0.3951, -0.4713), (-0.5854, -0.2474, 0.7721), (0.1885, 0.8847, 0.4264)85.8485, 87.0338, -48.5943
6given(-0.9999, -0.0043, 0.0153), (-0.0157, 0.4032, -0.915), (-0.0022, -0.9151, -0.4032)111.3426, 46.7781, 70.6
7given(0.7889, 0.5809, -0.2003), (0.585, -0.8098, -0.0445), (-0.1881, -0.0821, -0.9787)-16.1448, 64.0121, 45.1372
8given(-0.3178, 0.826, -0.4655), (0.8227, -0.0037, -0.5684), (-0.4713, -0.5636, -0.6784)36.5218, 15.9724, 82.3571
9given(0.2934, 0.7429, -0.6017), (-0.8255, 0.5143, 0.2324), (0.4821, 0.4285, 0.7641)8.8738, 67.6211, -45.3634

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Components

#1: Protein
5-CHLOROMUCONOLACTONE DEHALOGENASE / CHLORMUCONOLACTONE DEHALOGENASE


Mass: 11152.681 Da / Num. of mol.: 10 / Mutation: YES
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) RHODOCOCCUS OPACUS (bacteria) / Strain: 1CP / Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: Q8G9L0
#2: Chemical
ChemComp-K6H / (2S)-2-chloranyl-2-[(2R)-5-oxidanylidene-2H-furan-2-yl]ethanoic acid / 5-CHLOROMUCONOLACTONE


Mass: 176.554 Da / Num. of mol.: 10 / Source method: obtained synthetically / Formula: C6H5ClO4
#3: Chemical
ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 14 / Source method: obtained synthetically / Formula: Cl
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 494 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.48 Å3/Da / Density % sol: 46.6 % / Description: NONE

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: BESSY / Beamline: 14.1 / Wavelength: 0.91841
DetectorType: MARRESEARCH / Detector: CCD / Details: MIRRORS
RadiationMonochromator: DOUBLE CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.91841 Å / Relative weight: 1
ReflectionResolution: 2.22→20 Å / Num. obs: 102684 / % possible obs: 97.6 % / Observed criterion σ(I): -3 / Redundancy: 4.7 % / Biso Wilson estimate: 34.74 Å2 / Rmerge(I) obs: 0.05 / Net I/σ(I): 13.6
Reflection shellResolution: 2.22→2.36 Å / Redundancy: 4.5 % / Rmerge(I) obs: 0.47 / Mean I/σ(I) obs: 2.04 / % possible all: 91.2

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Processing

Software
NameVersionClassification
PHENIX(PHENIX.REFINE)refinement
XDSdata reduction
XDSdata scaling
MOLREPphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.224→19.935 Å / SU ML: 0.62 / σ(F): 1.99 / Phase error: 21.22 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2324 1100 2 %
Rwork0.1862 --
obs0.1872 54447 98.86 %
Solvent computationShrinkage radii: 0.98 Å / VDW probe radii: 1.2 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 34.56 Å2 / ksol: 0.341 e/Å3
Displacement parametersBiso mean: 39.8 Å2
Baniso -1Baniso -2Baniso -3
1-1.0351 Å20 Å20 Å2
2--0.6636 Å20 Å2
3----1.6987 Å2
Refinement stepCycle: LAST / Resolution: 2.224→19.935 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms7724 0 124 494 8342
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0028114
X-RAY DIFFRACTIONf_angle_d0.94711042
X-RAY DIFFRACTIONf_dihedral_angle_d13.1723095
X-RAY DIFFRACTIONf_chiral_restr0.0431176
X-RAY DIFFRACTIONf_plane_restr0.0031423
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.2243-2.32540.29691100.24856111X-RAY DIFFRACTION92
2.3254-2.44780.29741650.23686637X-RAY DIFFRACTION100
2.4478-2.60090.29041100.21736716X-RAY DIFFRACTION100
2.6009-2.80120.2381650.21916646X-RAY DIFFRACTION100
2.8012-3.08220.27831100.20826732X-RAY DIFFRACTION100
3.0822-3.5260.24761650.1956715X-RAY DIFFRACTION100
3.526-4.43440.19081100.15546809X-RAY DIFFRACTION100
4.4344-19.93540.19741650.15926981X-RAY DIFFRACTION100
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.2926-0.46070.01240.7161-0.02690.12170.21670.08810.00860.14050.156-0.0146-0.24690.15970.01610.2706-0.02080.01810.2533-0.00240.225139.556565.443122.2967
20.1223-0.1494-0.21150.21340.31140.5036-0.0081-0.03260.2968-0.0203-0.10040.1535-0.1584-0.6895-0.00330.31420.01510.10220.3090.00460.313628.837771.408525.9451
30.2681-0.0704-0.09480.27550.28860.30360.25990.0633-0.2350.2299-0.22160.0585-0.04170.17760.00120.2174-0.01970.02380.28130.03910.230732.066663.648814.6795
40.16640.08730.06860.33080.05270.2712-0.0123-0.14860.20380.2507-0.13790.2145-0.48760.3994-0.00780.23510.01560.03030.27520.03890.259238.260474.69314.9783
50.51580.2937-0.2620.3312-0.0420.1989-0.00750.09970.01410.09410.09910.3234-0.06830.20520.05420.1996-0.0028-0.03050.28130.03490.303937.411262.64328.6442
60.6647-0.5905-0.1740.57290.08850.67150.08130.413-0.0841-0.1757-0.08470.06670.1877-0.09310.00470.20110.0314-0.00860.2661-0.03650.175855.952561.6849-11.6992
70.35930.13820.20070.1237-0.00690.3613-0.2631-0.2039-0.18880.33170.24840.01340.07140.08-0.00010.20480.02490.01170.26120.00860.269457.76569.0349-4.5872
80.65730.2745-0.14590.34390.24950.44950.0610.15480.0222-0.15190.0516-0.05040.0442-0.056500.2020.0174-0.00290.2548-0.01060.222447.563864.064-4.5099
90.00460.03560.04120.37250.26170.51920.00250.22550.3891-0.10010.02450.5175-0.2886-0.4716-0.10070.18820.0027-0.02460.2060.10110.371149.633783.437.0263
100.37430.2494-0.17710.1626-0.11310.1964-0.19390.0306-0.4988-0.11730.0868-0.12830.4508-0.0243-0.00650.2175-0.0072-0.00480.21490.00420.186460.302873.08669.6432
110.68760.18230.02990.04890.00770.0016-0.5225-0.2821-0.25720.3780.2069-0.21120.21050.16770.05790.8930.09730.07270.27590.0360.302950.591773.890230.7477
120.0322-0.1091-0.09870.36540.32760.29090.1723-0.18310.22220.419-0.05770.358-0.71050.0517-0.00010.4128-0.03560.05870.3177-0.02850.460454.459986.042119.4545
130.4059-0.18470.2310.39090.01620.1708-0.1402-0.04550.0014-0.01540.09750.02130.15160.059100.2127-0.03620.02160.23130.00770.236956.652777.77048.6435
140.3924-0.2557-0.24790.4426-0.00730.24960.0326-0.13040.04140.2636-0.039-0.0711-0.16480.087400.2291-0.05190.00990.2650.01010.191464.144575.191215.7764
150.0335-0.01810.02430.019-0.02390.0298-0.02350.2738-0.1601-0.28930.0366-0.0499-0.20950.2891-0.00160.2525-0.0042-0.06260.25410.080.296361.438773.7151-8.6055
160.40870.46920.3170.52380.36870.26370.01530.05870.1497-0.11670.1625-0.18770.22360.211300.28830.01520.01830.3078-0.02330.292940.83746.87370.7827
170.1437-0.4845-0.21771.64040.74170.3359-0.01721.1031-0.1451-0.4245-0.23750.52440.3579-0.7395-0.17820.4362-0.1226-0.09470.66990.05180.324331.733544.7463-7.858
180.2719-0.13740.3760.5485-0.11510.52270.1647-0.1420.04480.0416-0.02920.2819-0.0155-0.023900.1876-0.035-0.0150.26350.00130.293632.271352.47074.341
190.1301-0.1706-0.00550.2219-0.00120.03450.11060.3361-0.3114-0.2347-0.18170.06190.4191-0.3870.00160.3447-0.07780.00440.35120.00760.340932.76339.72985.9908
200.0523-0.02230.02990.24820.19450.1837-0.04320.1902-0.04540.0456-0.0035-0.0280.1753-0.214100.2817-0.01560.06070.30960.03460.311134.340451.464612.1182
211.2879-0.464-0.64950.36650.59540.9747-0.11530.0986-0.37760.1382-0.0160.1856-0.2865-0.0688-0.10710.37630.026-0.0160.36660.03620.257270.493965.128127.5147
220.04-0.06630.21590.91680.35451.832-0.1214-0.11950.37850.06550.0362-0.2427-0.32450.4209-1.52410.5291-0.0966-0.3160.4113-0.06240.616181.309270.494230.4975
230.10450.1132-0.15840.3032-0.08970.2729-0.02960.1059-0.12240.24520.0065-0.1774-0.0260.30680.00450.2305-0.0677-0.07850.35720.05240.374679.037563.390320.4031
240.1098-0.0121-0.25380.2277-0.12320.6830.1276-0.5533-0.09230.47620.236-0.59330.00070.28060.07910.3974-0.0084-0.14450.46450.01710.406178.772756.997731.4089
250.1118-0.02970.17420.43520.0230.32420.02980.0187-0.23840.057-0.0568-0.2607-0.07020.1041-0.00150.30310.0241-0.00340.40080.05080.317276.992855.819318.1908
260.2637-0.2859-0.4920.43870.39351.0475-0.0499-0.133-0.01680.15150.0171-0.1226-0.1140.3993-0.00010.46640.0407-0.04510.38840.04580.228365.621248.385341.6945
270.25340.03330.25660.0355-0.06070.4833-0.0275-0.2175-0.03050.1481-0.0379-0.10420.07160.0644-00.39350.01140.02240.29050.05640.293663.676839.670533.838
280.00080.0159-0.0440.0908-0.24250.64580.1084-0.109-0.1295-0.1974-0.4313-0.26890.1246-0.1163-0.04590.2680.02580.06810.3081-0.02110.297571.657151.99231.3456
290.26210.09450.05131.634-0.41970.13050.18150.06280.0096-0.7369-0.3889-0.11030.06190.2153-0.09790.28550.0810.08370.37080.00970.356681.993751.2575-5.9461
300.2195-0.00090.01110.12-0.11890.26180.2207-0.2392-0.0347-0.2429-0.3572-0.13110.14310.4226-0.01790.21930.010.02520.28060.0130.312679.994155.17066.939
310.7576-0.14310.54150.73630.05940.41910.08670.05240.0174-0.1221-0.1244-0.06690.06150.116500.20840.0020.03780.2560.02250.267372.946461.27670.4494
320.6167-0.6595-0.32781.17270.05390.3633-0.16940.1662-0.0042-0.25610.2985-0.4032-0.28980.2982-0.17730.3627-0.2168-0.12440.39880.0130.582781.934572.074117.9823
330.1812-0.3655-0.060.69970.11930.02210.2070.15080.0407-0.1209-0.03410.01680.12340.21120.00010.43010.0160.00770.3085-0.00340.275548.970333.059211.1001
340.4396-0.13260.11360.35620.33410.7166-0.1690.30650.00960.32180.12120.1540.1170.09170.0040.3932-0.0542-0.03730.3055-0.01340.352142.633425.760512.818
350.44760.42750.06280.40670.0330.29080.09130.1187-0.1870.02590.1709-0.01450.09480.0064-00.38470.027-0.00220.22930.00440.288951.95426.853520.3386
360.04430.05540.00720.07530.05270.10440.02640.08210.06460.09290.0697-0.0440.4522-0.0893-00.42130.0310.03920.28780.07110.342248.192235.016425.3841
370.2859-0.1142-0.18610.10150.15380.2261-0.2240.1616-0.0108-0.022-0.01120.32960.18930.24880.00010.36870.0726-0.0390.34930.00080.345768.014636.002911.2286
380.77250.30481.40210.11920.53453.33280.07370.3474-0.8341-0.10330.3357-0.04470.65850.7802-0.02890.51440.08140.07620.2986-0.03270.608574.746925.327610.0909
390.1244-0.0406-0.20290.31020.06980.4497-0.0895-0.0390.0282-0.1263-0.06340.02410.1457-0.0764-0.00040.32230.0863-0.00740.33090.06150.394372.459234.303820.9969
400.05940.1148-0.0320.5615-0.17390.2176-0.0328-0.1187-0.1197-0.0729-0.1284-0.25920.0893-0.1029-00.3420.09910.00240.39030.02030.38575.637540.029712.104
410.0231-0.1054-0.22041.09630.92322.05960.0443-0.2426-0.22540.16410.2178-0.286-0.22580.38990.59280.37470.0397-0.14090.49980.10430.319376.134143.297536.5941
420.4172-0.3209-0.24350.60210.68670.8164-0.433-0.04670.1524-0.2270.297-0.032-0.62110.0474-0.01320.4589-0.0317-0.0070.236-0.01850.296643.547950.616132.7597
430.3301-0.15530.18431.24231.15481.421-0.2114-0.5026-0.19310.5852-0.17860.13880.2263-0.4781-0.10790.47760.04470.05440.34520.05590.284337.499647.68843.5427
440.8838-0.11660.52380.0181-0.07250.3184-0.18690.59960.2020.20060.12970.27530.4202-0.01730.00950.3271-0.04130.02380.24580.01090.422336.232338.301732.7137
450.2383-0.108-0.22630.08720.00080.4557-0.33140.0273-0.02440.30840.21350.0545-0.03840.1081-00.3197-0.03790.03470.28970.01990.356240.23542.703930.1083
460.1803-0.1371-0.04740.10640.05770.1104-0.2395-0.0013-0.08670.20660.34950.0573-0.67940.0263-0.00010.409-0.00590.09980.21310.03870.321431.768451.246630.0984
470.0692-0.14370.10980.3384-0.29230.2657-0.12840.13350.05710.09040.1640.01540.10240.3868-0.00010.4091-0.02440.00690.22620.0230.266840.49150.064327.0152
480.1222-0.3409-0.14510.94880.42920.43040.04610.0402-0.3653-0.286-0.33180.6719-0.3271-0.426-0.06050.3842-0.03050.06050.2353-0.0020.475135.315728.491716.3515
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1CHAIN A AND (RESSEQ 1:16)
2X-RAY DIFFRACTION2CHAIN A AND (RESSEQ 17:37)
3X-RAY DIFFRACTION3CHAIN A AND (RESSEQ 38:60)
4X-RAY DIFFRACTION4CHAIN A AND (RESSEQ 61:73)
5X-RAY DIFFRACTION5CHAIN A AND (RESSEQ 74:92)
6X-RAY DIFFRACTION6CHAIN B AND (RESSEQ 1:37)
7X-RAY DIFFRACTION7CHAIN B AND (RESSEQ 38:50)
8X-RAY DIFFRACTION8CHAIN B AND (RESSEQ 51:84)
9X-RAY DIFFRACTION9CHAIN B AND (RESSEQ 85:94)
10X-RAY DIFFRACTION10CHAIN C AND (RESSEQ 1:9)
11X-RAY DIFFRACTION11CHAIN C AND (RESSEQ 10:16)
12X-RAY DIFFRACTION12CHAIN C AND (RESSEQ 17:37)
13X-RAY DIFFRACTION13CHAIN C AND (RESSEQ 38:60)
14X-RAY DIFFRACTION14CHAIN C AND (RESSEQ 61:84)
15X-RAY DIFFRACTION15CHAIN C AND (RESSEQ 85:94)
16X-RAY DIFFRACTION16CHAIN D AND (RESSEQ 1:16)
17X-RAY DIFFRACTION17CHAIN D AND (RESSEQ 17:37)
18X-RAY DIFFRACTION18CHAIN D AND (RESSEQ 38:60)
19X-RAY DIFFRACTION19CHAIN D AND (RESSEQ 61:73)
20X-RAY DIFFRACTION20CHAIN D AND (RESSEQ 74:91)
21X-RAY DIFFRACTION21CHAIN E AND (RESSEQ 1:19)
22X-RAY DIFFRACTION22CHAIN E AND (RESSEQ 20:37)
23X-RAY DIFFRACTION23CHAIN E AND (RESSEQ 38:60)
24X-RAY DIFFRACTION24CHAIN E AND (RESSEQ 61:73)
25X-RAY DIFFRACTION25CHAIN E AND (RESSEQ 74:91)
26X-RAY DIFFRACTION26CHAIN F AND (RESSEQ 1:37)
27X-RAY DIFFRACTION27CHAIN F AND (RESSEQ 38:91)
28X-RAY DIFFRACTION28CHAIN G AND (RESSEQ 1:16)
29X-RAY DIFFRACTION29CHAIN G AND (RESSEQ 17:37)
30X-RAY DIFFRACTION30CHAIN G AND (RESSEQ 38:50)
31X-RAY DIFFRACTION31CHAIN G AND (RESSEQ 51:84)
32X-RAY DIFFRACTION32CHAIN G AND (RESSEQ 85:94)
33X-RAY DIFFRACTION33CHAIN H AND (RESSEQ 1:16)
34X-RAY DIFFRACTION34CHAIN H AND (RESSEQ 17:50)
35X-RAY DIFFRACTION35CHAIN H AND (RESSEQ 51:73)
36X-RAY DIFFRACTION36CHAIN H AND (RESSEQ 74:92)
37X-RAY DIFFRACTION37CHAIN I AND (RESSEQ 1:16)
38X-RAY DIFFRACTION38CHAIN I AND (RESSEQ 17:37)
39X-RAY DIFFRACTION39CHAIN I AND (RESSEQ 38:60)
40X-RAY DIFFRACTION40CHAIN I AND (RESSEQ 61:83)
41X-RAY DIFFRACTION41CHAIN I AND (RESSEQ 84:92)
42X-RAY DIFFRACTION42CHAIN J AND (RESSEQ 1:16)
43X-RAY DIFFRACTION43CHAIN J AND (RESSEQ 17:36)
44X-RAY DIFFRACTION44CHAIN J AND (RESSEQ 37:45)
45X-RAY DIFFRACTION45CHAIN J AND (RESSEQ 46:60)
46X-RAY DIFFRACTION46CHAIN J AND (RESSEQ 61:73)
47X-RAY DIFFRACTION47CHAIN J AND (RESSEQ 74:84)
48X-RAY DIFFRACTION48CHAIN J AND (RESSEQ 85:92)

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