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- PDB-3zkj: Crystal Structure of Ankyrin Repeat and Socs Box-Containing Prote... -

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Basic information

Entry
Database: PDB / ID: 3zkj
TitleCrystal Structure of Ankyrin Repeat and Socs Box-Containing Protein 9 (Asb9) in Complex with Elonginb and Elonginc
Components
  • (TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE ...) x 2
  • ANKYRIN REPEAT AND SOCS BOX PROTEIN 9
KeywordsTRANSCRIPTION / TRANSCRIPTION REGULATION / AUTOANTIBODY
Function / homology
Function and homology information


target-directed miRNA degradation / elongin complex / VCB complex / Cul5-RING ubiquitin ligase complex / Cul2-RING ubiquitin ligase complex / Pausing and recovery of Tat-mediated HIV elongation / Tat-mediated HIV elongation arrest and recovery / HIV elongation arrest and recovery / Pausing and recovery of HIV elongation / ubiquitin-like ligase-substrate adaptor activity ...target-directed miRNA degradation / elongin complex / VCB complex / Cul5-RING ubiquitin ligase complex / Cul2-RING ubiquitin ligase complex / Pausing and recovery of Tat-mediated HIV elongation / Tat-mediated HIV elongation arrest and recovery / HIV elongation arrest and recovery / Pausing and recovery of HIV elongation / ubiquitin-like ligase-substrate adaptor activity / Tat-mediated elongation of the HIV-1 transcript / Formation of HIV-1 elongation complex containing HIV-1 Tat / Formation of HIV elongation complex in the absence of HIV Tat / RNA Polymerase II Transcription Elongation / Formation of RNA Pol II elongation complex / RNA Polymerase II Pre-transcription Events / transcription corepressor binding / TP53 Regulates Transcription of DNA Repair Genes / transcription initiation at RNA polymerase II promoter / transcription elongation by RNA polymerase II / Vif-mediated degradation of APOBEC3G / Inactivation of CSF3 (G-CSF) signaling / Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha / Evasion by RSV of host interferon responses / Regulation of expression of SLITs and ROBOs / positive regulation of protein catabolic process / Antigen processing: Ubiquitination & Proteasome degradation / positive regulation of proteasomal ubiquitin-dependent protein catabolic process / Neddylation / protein-macromolecule adaptor activity / ubiquitin-dependent protein catabolic process / protein-containing complex assembly / proteasome-mediated ubiquitin-dependent protein catabolic process / intracellular signal transduction / protein ubiquitination / ubiquitin protein ligase binding / regulation of transcription by RNA polymerase II / mitochondrion / nucleoplasm / cytosol
Similarity search - Function
Ankyrin repeat and SOCS box protein 9/11, SOCS box domain / : / SOCS box / SOCS box / SOCS box-like domain superfamily / SOCS box domain / SOCS box domain profile. / SOCS_box / Elongin C; Chain C, domain 1 / Elongin B ...Ankyrin repeat and SOCS box protein 9/11, SOCS box domain / : / SOCS box / SOCS box / SOCS box-like domain superfamily / SOCS box domain / SOCS box domain profile. / SOCS_box / Elongin C; Chain C, domain 1 / Elongin B / Elongin-C / Potassium Channel Kv1.1; Chain A / Potassium Channel Kv1.1; Chain A / Ankyrin repeat-containing domain / S-phase kinase-associated protein 1-like / SKP1 component, POZ domain / Skp1 family, tetramerisation domain / Found in Skp1 protein family / SKP1/BTB/POZ domain superfamily / Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1 / Ankyrin repeats (3 copies) / Ankyrin repeat profile. / Ankyrin repeat region circular profile. / ankyrin repeats / Ankyrin repeat / Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat / Ankyrin repeat-containing domain superfamily / Ubiquitin-like (UB roll) / Alpha Horseshoe / Ubiquitin family / Ubiquitin homologues / Ubiquitin domain profile. / Ubiquitin-like domain / Ubiquitin-like domain superfamily / Roll / 2-Layer Sandwich / Orthogonal Bundle / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
DI(HYDROXYETHYL)ETHER / Elongin-C / Elongin-B / Ankyrin repeat and SOCS box protein 9
Similarity search - Component
Biological speciesHOMO SAPIENS (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.58 Å
AuthorsMuniz, J.R.C. / Guo, K. / Zhang, Y. / Ayinampudi, V. / Savitsky, P. / Keates, T. / Filippakopoulos, P. / Vollmar, M. / Yue, W.W. / Krojer, T. ...Muniz, J.R.C. / Guo, K. / Zhang, Y. / Ayinampudi, V. / Savitsky, P. / Keates, T. / Filippakopoulos, P. / Vollmar, M. / Yue, W.W. / Krojer, T. / Ugochukwu, E. / von Delft, F. / Knapp, S. / Weigelt, J. / Arrowsmith, C.H. / Edwards, A.M. / Bountra, C. / Bullock, A.N.
CitationJournal: J.Mol.Biol. / Year: 2013
Title: Molecular Architecture of the Ankyrin Socs Box Family of Cul5-Dependent E3 Ubiquitin Ligases
Authors: Muniz, J.R.C. / Guo, K. / Kershaw, N.J. / Ayinampudi, V. / von Delft, F. / Babon, J.J. / Bullock, A.N.
History
DepositionJan 23, 2013Deposition site: PDBE / Processing site: PDBE
SupersessionJan 30, 2013ID: 2XAI
Revision 1.0Jan 30, 2013Provider: repository / Type: Initial release
Revision 1.1Jul 3, 2013Group: Database references / Other ...Database references / Other / Refinement description / Structure summary
Revision 1.2Jul 10, 2013Group: Database references
Revision 1.3Aug 28, 2013Group: Database references
Revision 1.4Jan 24, 2018Group: Database references / Category: citation_author / Item: _citation_author.name
Revision 1.5May 8, 2024Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Other / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_database_status / struct_ncs_dom_lim / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.status_code_sf / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: ANKYRIN REPEAT AND SOCS BOX PROTEIN 9
B: TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 1
C: TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 2
D: ANKYRIN REPEAT AND SOCS BOX PROTEIN 9
E: TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 1
F: TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)105,40311
Polymers105,1026
Non-polymers3015
Water1,11762
1
A: ANKYRIN REPEAT AND SOCS BOX PROTEIN 9
B: TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 1
C: TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)52,7556
Polymers52,5513
Non-polymers2043
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4570 Å2
ΔGint-44.2 kcal/mol
Surface area19940 Å2
MethodPISA
2
D: ANKYRIN REPEAT AND SOCS BOX PROTEIN 9
E: TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 1
F: TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)52,6495
Polymers52,5513
Non-polymers982
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4560 Å2
ΔGint-54.6 kcal/mol
Surface area20550 Å2
MethodPISA
Unit cell
Length a, b, c (Å)102.860, 110.740, 113.410
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number18
Space group name H-MP21221
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11A
21D
12B
22E

NCS domain segments:

Component-ID: _ / Refine code: _

Dom-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11TRPTRPLEULEUAA37 - 2944 - 261
21TRPTRPLEULEUDD37 - 2944 - 261
12TYRTYRCYSCYSBB18 - 1122 - 96
22TYRTYRCYSCYSEE18 - 1122 - 96

NCS ensembles :
ID
1
2

NCS oper: (Code: given
Matrix: (0.0843, -0.0303, -0.996), (-0.0109, -0.9995, 0.0295), (-0.9964, 0.0083, -0.0846)
Vector: 41.8607, 6.9597, 46.2477)

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Components

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Protein , 1 types, 2 molecules AD

#1: Protein ANKYRIN REPEAT AND SOCS BOX PROTEIN 9 / ASB-9


Mass: 28559.803 Da / Num. of mol.: 2 / Fragment: RESIDUES 35-294
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human) / Organ: KIDNEY / Plasmid: P11 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21 / References: UniProt: Q96DX5

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TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE ... , 2 types, 4 molecules BECF

#2: Protein TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 1 / RNA POLYMERASE II TRANSCRIPTION FACTOR / ELOC / SIII SUBUNIT C / SIII P15 / ELONGIN 15 KDA SUBUNIT / ELONGIN-C


Mass: 10843.420 Da / Num. of mol.: 2 / Fragment: RESIDUES 17-112
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human) / Organ: KIDNEY / Plasmid: P11 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21 / References: UniProt: Q15369
#3: Protein TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 2 / RNA POLYMERASE II TRANSCRIPTION FACTOR SIII SUBUNIT B / SIII P18 / ELONGIN-B / ELOB / ELONGIN 18 KDA SUBUNIT


Mass: 13147.781 Da / Num. of mol.: 2 / Fragment: RESIDUES 221-338
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human) / Organ: KIDNEY / Plasmid: P11 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21 / References: UniProt: Q15370

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Non-polymers , 4 types, 67 molecules

#4: Chemical ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL


Mass: 62.068 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C2H6O2
#5: Chemical ChemComp-PEG / DI(HYDROXYETHYL)ETHER


Mass: 106.120 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C4H10O3
#6: Chemical ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Cl
#7: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 62 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.81 Å3/Da / Density % sol: 67.78 % / Description: NONE
Crystal growDetails: 0.2M NA2SO4, 0.1M BTPROP 6.5, 20% PEG 3350, 10% ETGLY

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I02 / Wavelength: 0.9795
DetectorDate: Feb 27, 2010
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9795 Å / Relative weight: 1
ReflectionResolution: 2.58→50.47 Å / Num. obs: 41297 / % possible obs: 99.7 % / Observed criterion σ(I): 2.1 / Redundancy: 6.7 % / Rmerge(I) obs: 0.1 / Net I/σ(I): 10.7
Reflection shellResolution: 2.58→2.72 Å / Redundancy: 6.3 % / Rmerge(I) obs: 0.77 / Mean I/σ(I) obs: 2.1 / % possible all: 99.7

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Processing

Software
NameVersionClassification
REFMAC5.7.0029refinement
iMOSFLMdata reduction
SCALAdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.58→50.47 Å / Cor.coef. Fo:Fc: 0.938 / Cor.coef. Fo:Fc free: 0.913 / SU B: 23.907 / SU ML: 0.236 / Cross valid method: THROUGHOUT / ESU R: 0.418 / ESU R Free: 0.284 / Stereochemistry target values: MAXIMUM LIKELIHOOD
Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES WITH TLS ADDED
RfactorNum. reflection% reflectionSelection details
Rfree0.26507 2050 5 %RANDOM
Rwork0.22706 ---
obs0.2289 39120 99.4 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK
Displacement parametersBiso mean: 74.17 Å2
Baniso -1Baniso -2Baniso -3
1-2.2 Å20 Å20 Å2
2---3.31 Å20 Å2
3---1.12 Å2
Refinement stepCycle: LAST / Resolution: 2.58→50.47 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms6378 0 17 62 6457
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0140.0196541
X-RAY DIFFRACTIONr_bond_other_d0.0050.026200
X-RAY DIFFRACTIONr_angle_refined_deg1.521.9668886
X-RAY DIFFRACTIONr_angle_other_deg1.005314258
X-RAY DIFFRACTIONr_dihedral_angle_1_deg5.85843
X-RAY DIFFRACTIONr_dihedral_angle_2_deg34.61824.466253
X-RAY DIFFRACTIONr_dihedral_angle_3_deg15.698151036
X-RAY DIFFRACTIONr_dihedral_angle_4_deg14.5341525
X-RAY DIFFRACTIONr_chiral_restr0.0740.21037
X-RAY DIFFRACTIONr_gen_planes_refined0.0080.0217359
X-RAY DIFFRACTIONr_gen_planes_other0.0050.021400
X-RAY DIFFRACTIONr_nbd_refined
X-RAY DIFFRACTIONr_nbd_other
X-RAY DIFFRACTIONr_nbtor_refined
X-RAY DIFFRACTIONr_nbtor_other
X-RAY DIFFRACTIONr_xyhbond_nbd_refined
X-RAY DIFFRACTIONr_xyhbond_nbd_other
X-RAY DIFFRACTIONr_metal_ion_refined
X-RAY DIFFRACTIONr_metal_ion_other
X-RAY DIFFRACTIONr_symmetry_vdw_refined
X-RAY DIFFRACTIONr_symmetry_vdw_other
X-RAY DIFFRACTIONr_symmetry_hbond_refined
X-RAY DIFFRACTIONr_symmetry_hbond_other
X-RAY DIFFRACTIONr_symmetry_metal_ion_refined
X-RAY DIFFRACTIONr_symmetry_metal_ion_other
X-RAY DIFFRACTIONr_mcbond_it
X-RAY DIFFRACTIONr_mcbond_other
X-RAY DIFFRACTIONr_mcangle_it
X-RAY DIFFRACTIONr_mcangle_other
X-RAY DIFFRACTIONr_scbond_it
X-RAY DIFFRACTIONr_scbond_other
X-RAY DIFFRACTIONr_scangle_it
X-RAY DIFFRACTIONr_scangle_other
X-RAY DIFFRACTIONr_long_range_B_refined
X-RAY DIFFRACTIONr_long_range_B_other
X-RAY DIFFRACTIONr_rigid_bond_restr
X-RAY DIFFRACTIONr_sphericity_free
X-RAY DIFFRACTIONr_sphericity_bonded
Refine LS restraints NCS

Refine-ID: X-RAY DIFFRACTION / Type: interatomic distance / Weight position: 0.05

Ens-IDDom-IDAuth asym-IDNumberRms dev position (Å)
11A145450.09
12D145450.09
21B40960.06
22E40960.06
LS refinement shellResolution: 2.581→2.648 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.47 140 -
Rwork0.362 2852 -
obs--99.5 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
14.43451.10521.38396.4763.25984.3797-0.2344-0.40060.05640.28060.2022-0.08910.20150.13050.03220.4348-0.00880.04360.6119-0.05890.439622.10216.286950.7847
23.3550.33341.71582.77791.21672.32830.0806-0.5035-0.35140.3549-0.00150.24530.3378-0.2984-0.07910.4448-0.09040.07570.53180.09630.68069.2734-15.672535.4529
32.7699-1.367-1.54623.13111.84243.5120.30150.37430.23530.353-0.07920.46870.0538-0.3753-0.22240.4099-0.00330.04710.4070.14740.645914.7671-19.813419.7332
43.58542.16067.18671.56234.42614.450.63120.2635-0.6879-0.13040.6049-0.24781.05390.6445-1.23611.1507-0.7238-0.62061.0097-0.19191.038813.2103-36.98580.3683
538.7833-6.717420.562911.1826.369220.7564-0.3440.6702-0.15520.8951.163-0.81680.68661.5283-0.8190.9739-0.12240.210.6319-0.33460.620719.111-33.476-4.5292
612.11543.6378-4.80623.5321-0.46569.39460.21810.4491-0.43770.34320.10440.52190.7508-0.7364-0.32250.7162-0.1846-0.19290.4695-0.01670.850612.3888-36.150112.086
78.30682.77224.89389.42123.203111.1014-0.16130.7165-1.2749-0.02350.7301-0.323-0.0082-0.4154-0.56880.3536-0.0349-0.00120.45210.00930.598419.1803-28.944112.0787
83.74562.71052.2463.65651.74551.35591.04150.5178-2.26760.16610.2766-1.22650.60640.2905-1.3181.3226-0.331-0.36970.8966-0.48191.692116.4864-47.66096.8621
917.34869.2464-6.06149.0619-2.69812.6006-0.26842.1344-3.3776-0.3394-0.2271-2.19570.7221-1.58980.49561.3742-1.1005-0.5941.83040.58092.28177.7639-55.39710.1054
1011.369417.1247.652227.64145.331826.041-0.60211.7871-1.4685-1.5242.9565-1.66381.46260.4471-2.35441.8356-0.4578-0.29061.0455-0.65193.03059.5451-56.95592.6684
112.42683.9949-4.46396.6328-7.49478.6756-0.15240.3435-0.2546-0.4350.5506-0.13760.6605-0.7147-0.39821.3047-0.2366-0.74490.6283-0.14071.68316.0672-56.41417.1557
1213.6864-5.42320.82512.3454-0.34160.05380.3476-0.2939-0.4196-0.2474-0.27140.07450.05270.0058-0.07621.5412-0.3376-0.36440.97650.2751.152914.1423-45.872616.7471
138.82288.04332.751514.57846.56357.90480.55070.5394-1.11660.03270.8283-3.27861.02840.4184-1.37910.7560.10430.02190.4091-0.04581.16930.5279-33.042814.1829
145.40840.19287.14623.1424-0.858713.37160.08050.1417-0.15870.3533-0.20580.2097-0.1799-0.73250.12530.3636-0.10960.07850.4933-0.19580.5713-10.1965-8.510320.6288
155.8012.77172.00066.36972.53553.869-0.10120.32330.3027-0.3696-0.07740.2415-0.4215-0.16910.17870.37390.0407-0.02480.4657-0.03240.5094-4.85234.645818.8708
162.63842.60842.06233.70781.77124.57470.0062-0.15880.37230.12350.08310.3034-0.0481-0.2385-0.08940.57570.0566-0.02630.3352-0.00050.72336.643420.887729.8307
172.84650.9884.52042.4722-1.04310.8086-0.4089-0.01430.46540.25980.1130.1554-1.0080.18980.2960.5210.0790.02030.3601-0.3160.946115.104130.692942.838
181.41281.86390.64613.6332-1.14853.74620.0018-0.0002-0.3666-0.074-0.0586-0.69620.1710.12160.05680.57350.09520.07150.20040.13290.831326.032828.125822.7129
193.18353.4794-1.72154.4411-2.00890.95640.2471-0.1546-0.0260.9966-0.3683-1.3043-0.33140.09110.12121.12080.0759-0.72660.35260.20332.860139.249150.421528.8977
202.2382.0557-0.92752.71841.065.80410.16380.0702-0.09750.6139-0.0445-0.56960.75950.2956-0.11930.9457-0.0001-0.13990.57590.24391.794140.533645.1824.549
215.44832.70392.00566.43-0.97411.5232-0.1119-0.52180.37710.8171-0.0599-0.2502-0.3834-0.23240.17180.9910.1187-0.01080.48380.1031.204125.156945.538230.3014
220.80871.4376-0.791317.22144.42384.5694-0.2347-0.02740.33060.25670.4491-1.3670.0230.1881-0.21440.83560.18910.04670.40320.07751.370734.947136.871425.0645
231.9698-0.4076-1.12157.2525-0.84843.9427-0.1805-0.0849-0.42831.6859-0.4197-1.68420.03920.23880.60020.84580.0218-0.31430.15940.15171.143831.552463.752927.7824
2419.96059.5471.186316.6256-3.04868.9364-0.0110.7043-2.42361.153-0.4604-2.76850.1790.1390.47140.53170.0862-0.13020.2672-0.04941.634733.847170.765720.7145
250.81782.30680.254714.4156-1.0622.4135-0.33630.0692-0.26260.79910.4268-0.05510.1555-0.3238-0.09050.8714-0.02720.02920.31230.06310.853622.170863.596225.0777
260.88326.00020.956843.23066.44551.0880.22080.02620.37511.0677-0.74180.39650.06320.19230.5210.9196-0.1834-0.09281.02470.52392.972238.991571.068818.1588
276.717813.33140.802229.03851.29182.1219-0.9570.4004-0.8628-1.34031.0089-3.89530.11750.0973-0.0520.58350.1633-0.09650.25130.05242.408136.507864.359713.449
288.3232.4439-2.290320.07890.14780.6996-0.17910.4565-0.0004-2.1481-0.015-0.05970.0026-0.02720.1940.8320.06570.22120.61180.36740.985225.19634.241410.2921
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1A37 - 131
2X-RAY DIFFRACTION2A132 - 238
3X-RAY DIFFRACTION3A239 - 294
4X-RAY DIFFRACTION4B18 - 46
5X-RAY DIFFRACTION5B58 - 64
6X-RAY DIFFRACTION6B65 - 91
7X-RAY DIFFRACTION7B92 - 112
8X-RAY DIFFRACTION8C2 - 15
9X-RAY DIFFRACTION9C16 - 25
10X-RAY DIFFRACTION10C26 - 32
11X-RAY DIFFRACTION11C33 - 64
12X-RAY DIFFRACTION12C65 - 74
13X-RAY DIFFRACTION13C75 - 105
14X-RAY DIFFRACTION14D36 - 58
15X-RAY DIFFRACTION15D59 - 131
16X-RAY DIFFRACTION16D132 - 208
17X-RAY DIFFRACTION17D209 - 253
18X-RAY DIFFRACTION18D254 - 294
19X-RAY DIFFRACTION19E18 - 42
20X-RAY DIFFRACTION20E43 - 72
21X-RAY DIFFRACTION21E73 - 99
22X-RAY DIFFRACTION22E100 - 112
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