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- PDB-3zge: Greater efficiency of photosynthetic carbon fixation due to singl... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3zge | ||||||
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Title | Greater efficiency of photosynthetic carbon fixation due to single amino acid substitution | ||||||
![]() | C4 PHOSPHOENOLPYRUVATE CARBOXYLASE | ||||||
![]() | LYASE / C4 PHOTOSYNTHETIC PATHWAY / CARBON FIXATION / PEP CARBOXYLASE | ||||||
Function / homology | ![]() C4 photosynthesis / phosphoenolpyruvate carboxylase / phosphoenolpyruvate carboxylase activity / leaf development / carbon fixation / apoplast / tricarboxylic acid cycle / chloroplast / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Paulus, J.K. / Schlieper, D. / Groth, G. | ||||||
![]() | ![]() Title: Greater Efficiency of Photosynthetic Carbon Fixation due to Single Amino Acid Substitution Authors: Paulus, J.K. / Schlieper, D. / Groth, G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 689.6 KB | Display | ![]() |
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PDB format | ![]() | 569.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 3zgbC ![]() 1jqoS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 113486.578 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: P30694, phosphoenolpyruvate carboxylase #2: Chemical | #3: Chemical | #4: Chemical | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.1 Å3/Da / Density % sol: 60 % / Description: NONE |
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Crystal grow | pH: 5.6 Details: 0.2 M AMMONIUM SULFATE, 0.1 M TRI-SODIUM CITRATE/HCL PH 5.6, 15 % PEG 4000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Jun 11, 2010 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9763 Å / Relative weight: 1 |
Reflection | Resolution: 2.49→37.83 Å / Num. obs: 90203 / % possible obs: 100 % / Observed criterion σ(I): 2 / Redundancy: 26.2 % / Biso Wilson estimate: 45.2 Å2 / Rsym value: 0.097 / Net I/σ(I): 5.9 |
Reflection shell | Resolution: 2.49→2.62 Å / Redundancy: 24 % / Mean I/σ(I) obs: 5.9 / Rsym value: 0.389 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1JQO Resolution: 2.49→37 Å / Cor.coef. Fo:Fc: 0.937 / Cor.coef. Fo:Fc free: 0.918 / SU B: 13.145 / SU ML: 0.171 / Cross valid method: THROUGHOUT / ESU R: 0.358 / ESU R Free: 0.242 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES WITH TLS ADDED
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 39.521 Å2
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Refinement step | Cycle: LAST / Resolution: 2.49→37 Å
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Refine LS restraints |
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