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- PDB-3zg5: Crystal structure of PBP2a from MRSA in complex with peptidoglyca... -

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Basic information

Entry
Database: PDB / ID: 3zg5
TitleCrystal structure of PBP2a from MRSA in complex with peptidoglycan analogue at allosteric
Components
  • PEPTIDOGLYCAN ANALOGUE
  • Penicillin binding protein 2 prime
KeywordsHYDROLASE / PENICILLIN BINDING PROTEINS / ALLOSTERIC SITE / B- LACTAM ANTIBIOTICS
Function / homology
Function and homology information


peptidoglycan L,D-transpeptidase activity / penicillin binding / cell wall organization / response to antibiotic / plasma membrane
Similarity search - Function
Penicillin-binding protein 2a; domain 3 / NTF2-like; domain 1 / NTF2-like N-terminal transpeptidase / NTF2-like N-terminal transpeptidase domain / Penicillin-binding protein 2a (Domain 2) / Penicillin-binding protein 2a (Domain 2) / Ribosomal Protein L30; Chain: A, / Penicillin-binding protein, dimerisation domain / Penicillin-binding Protein dimerisation domain / Penicillin-binding protein, dimerisation domain superfamily ...Penicillin-binding protein 2a; domain 3 / NTF2-like; domain 1 / NTF2-like N-terminal transpeptidase / NTF2-like N-terminal transpeptidase domain / Penicillin-binding protein 2a (Domain 2) / Penicillin-binding protein 2a (Domain 2) / Ribosomal Protein L30; Chain: A, / Penicillin-binding protein, dimerisation domain / Penicillin-binding Protein dimerisation domain / Penicillin-binding protein, dimerisation domain superfamily / : / Penicillin-binding protein, transpeptidase / Penicillin binding protein transpeptidase domain / NTF2-like domain superfamily / Nuclear Transport Factor 2; Chain: A, / Beta-lactamase / DD-peptidase/beta-lactamase superfamily / Beta-lactamase/transpeptidase-like / Roll / Alpha-Beta Complex / 2-Layer Sandwich / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
Chem-AMV / : / Penicillin binding protein 2 prime
Similarity search - Component
Biological speciesStaphylococcus aureus subsp. aureus Mu50 (bacteria)
SYNTHETIC CONSTRUCT (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.55 Å
AuthorsOtero, L.H. / Rojas-Altuve, A. / Hermoso, J.A.
CitationJournal: Proc.Natl.Acad.Sci.USA / Year: 2013
Title: How Allosteric Control of Staphylococcus Aureus Penicillin Binding Protein 2A Enables Methicillin Resistance and Physiological Function
Authors: Otero, L.H. / Rojas-Altuve, A. / Llarrull, L.I. / Carrasco-Lopez, C. / Kumarasiri, M. / Lastochkin, E. / Fishovitz, J. / Dawley, M. / Hesek, D. / Lee, M. / Johnson, J.W. / Fisher, J.F. / ...Authors: Otero, L.H. / Rojas-Altuve, A. / Llarrull, L.I. / Carrasco-Lopez, C. / Kumarasiri, M. / Lastochkin, E. / Fishovitz, J. / Dawley, M. / Hesek, D. / Lee, M. / Johnson, J.W. / Fisher, J.F. / Chang, M. / Mobashery, S. / Hermoso, J.A.
History
DepositionDec 14, 2012Deposition site: PDBE / Processing site: PDBE
Revision 1.0Oct 9, 2013Provider: repository / Type: Initial release
Revision 1.1Oct 30, 2013Group: Database references
Revision 1.2Dec 14, 2016Group: Source and taxonomy
Revision 1.3Jul 29, 2020Group: Advisory / Derived calculations ...Advisory / Derived calculations / Other / Structure summary
Category: chem_comp / pdbx_database_status ...chem_comp / pdbx_database_status / pdbx_struct_conn_angle / pdbx_validate_polymer_linkage / struct_conn / struct_conn_type / struct_site / struct_site_gen
Item: _chem_comp.mon_nstd_flag / _chem_comp.name ..._chem_comp.mon_nstd_flag / _chem_comp.name / _chem_comp.type / _pdbx_database_status.status_code_sf / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.conn_type_id / _struct_conn.id / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_conn_type.id
Description: Carbohydrate remediation / Provider: repository / Type: Remediation
Revision 2.0Dec 7, 2022Group: Advisory / Atomic model ...Advisory / Atomic model / Data collection / Database references / Derived calculations / Polymer sequence / Source and taxonomy / Structure summary
Category: atom_site / chem_comp ...atom_site / chem_comp / database_2 / entity / entity_name_com / entity_poly / entity_poly_seq / entity_src_gen / pdbx_distant_solvent_atoms / pdbx_entity_nonpoly / pdbx_entity_src_syn / pdbx_nonpoly_scheme / pdbx_poly_seq_scheme / pdbx_struct_assembly_gen / pdbx_struct_conn_angle / pdbx_validate_close_contact / pdbx_validate_symm_contact / struct_asym / struct_conn / struct_mon_prot_cis / struct_ref / struct_ref_seq
Item: _atom_site.B_iso_or_equiv / _atom_site.Cartn_x ..._atom_site.B_iso_or_equiv / _atom_site.Cartn_x / _atom_site.Cartn_y / _atom_site.Cartn_z / _atom_site.auth_asym_id / _atom_site.auth_atom_id / _atom_site.auth_comp_id / _atom_site.auth_seq_id / _atom_site.group_PDB / _atom_site.label_asym_id / _atom_site.label_atom_id / _atom_site.label_comp_id / _atom_site.label_entity_id / _atom_site.label_seq_id / _atom_site.type_symbol / _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _entity_poly.pdbx_seq_one_letter_code / _entity_poly.pdbx_seq_one_letter_code_can / _entity_src_gen.pdbx_beg_seq_num / _entity_src_gen.pdbx_end_seq_num / _entity_src_gen.pdbx_gene_src_gene / _entity_src_gen.pdbx_gene_src_scientific_name / _entity_src_gen.pdbx_seq_type / _pdbx_distant_solvent_atoms.auth_seq_id / _pdbx_entity_src_syn.pdbx_beg_seq_num / _pdbx_entity_src_syn.pdbx_end_seq_num / _pdbx_struct_assembly_gen.asym_id_list / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_validate_close_contact.auth_asym_id_1 / _pdbx_validate_close_contact.auth_asym_id_2 / _pdbx_validate_close_contact.auth_atom_id_1 / _pdbx_validate_close_contact.auth_atom_id_2 / _pdbx_validate_close_contact.auth_comp_id_1 / _pdbx_validate_close_contact.auth_comp_id_2 / _pdbx_validate_close_contact.auth_seq_id_1 / _pdbx_validate_close_contact.auth_seq_id_2 / _pdbx_validate_close_contact.dist / _pdbx_validate_symm_contact.auth_asym_id_1 / _pdbx_validate_symm_contact.auth_asym_id_2 / _pdbx_validate_symm_contact.auth_atom_id_1 / _pdbx_validate_symm_contact.auth_comp_id_1 / _pdbx_validate_symm_contact.auth_seq_id_1 / _pdbx_validate_symm_contact.auth_seq_id_2 / _pdbx_validate_symm_contact.dist / _pdbx_validate_symm_contact.site_symmetry_2 / _struct_mon_prot_cis.label_seq_id / _struct_mon_prot_cis.pdbx_label_seq_id_2 / _struct_ref.db_code / _struct_ref.pdbx_align_begin / _struct_ref.pdbx_db_accession / _struct_ref.pdbx_seq_one_letter_code / _struct_ref_seq.db_align_beg / _struct_ref_seq.pdbx_auth_seq_align_beg / _struct_ref_seq.pdbx_db_accession / _struct_ref_seq.seq_align_end
Revision 2.1Jul 5, 2023Group: Advisory / Derived calculations / Category: pdbx_validate_close_contact / struct_conn
Item: _struct_conn.pdbx_dist_value / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.ptnr1_label_atom_id
Revision 2.2Feb 7, 2024Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model
Revision 2.3Nov 13, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Penicillin binding protein 2 prime
B: Penicillin binding protein 2 prime
C: PEPTIDOGLYCAN ANALOGUE
D: PEPTIDOGLYCAN ANALOGUE
hetero molecules


Theoretical massNumber of molelcules
Total (without water)148,75814
Polymers147,5524
Non-polymers1,20610
Water3,891216
1
A: Penicillin binding protein 2 prime
C: PEPTIDOGLYCAN ANALOGUE
hetero molecules


Theoretical massNumber of molelcules
Total (without water)74,3797
Polymers73,7762
Non-polymers6035
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1680 Å2
ΔGint-34.6 kcal/mol
Surface area29340 Å2
MethodPISA
2
B: Penicillin binding protein 2 prime
D: PEPTIDOGLYCAN ANALOGUE
hetero molecules


Theoretical massNumber of molelcules
Total (without water)74,3797
Polymers73,7762
Non-polymers6035
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1670 Å2
ΔGint-32.2 kcal/mol
Surface area29540 Å2
MethodPISA
Unit cell
Length a, b, c (Å)80.540, 102.020, 185.980
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121

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Components

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Protein / Protein/peptide / Sugars , 3 types, 6 molecules ABCD

#1: Protein Penicillin binding protein 2 prime


Mass: 73286.656 Da / Num. of mol.: 2 / Fragment: RESIDUES 27-668
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Staphylococcus aureus subsp. aureus Mu50 (bacteria)
Strain: STRAIN MU50 / Gene: mecA, SAV0041 / Plasmid: PET24D / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): STAR / References: UniProt: A0A0H3JPA5
#2: Protein/peptide PEPTIDOGLYCAN ANALOGUE


Mass: 489.542 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) SYNTHETIC CONSTRUCT (others)
#5: Sugar ChemComp-AMV / methyl 2-acetamido-3-O-[(1R)-1-carboxyethyl]-2-deoxy-beta-D-glucopyranoside / METHYL 2-(ACETYLAMINO)-3-O-[(1R)-1-CARBOXYETHYL]-2-DEOXY-BETA-D-GLUCOPYRANOSIDE / methyl 2-acetamido-3-O-[(1R)-1-carboxyethyl]-2-deoxy-beta-D-glucoside / methyl 2-acetamido-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucoside / methyl 2-acetamido-3-O-[(1R)-1-carboxyethyl]-2-deoxy-glucoside


Type: D-saccharide / Mass: 307.297 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Formula: C12H21NO8

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Non-polymers , 3 types, 224 molecules

#3: Chemical
ChemComp-CD / CADMIUM ION


Mass: 112.411 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Cd
#4: Chemical
ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Cl
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 216 / Source method: isolated from a natural source / Formula: H2O

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Details

Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION

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Sample preparation

CrystalDensity Matthews: 2.59 Å3/Da / Density % sol: 52.51 % / Description: NONE
Crystal growpH: 7 / Details: pH 7

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: ESRF / Beamline: ID29 / Wavelength: 0.9334
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9334 Å / Relative weight: 1
ReflectionResolution: 2.55→49.13 Å / Num. obs: 47511 / % possible obs: 94.3 % / Observed criterion σ(I): 2 / Redundancy: 4 % / Biso Wilson estimate: 32.79 Å2 / Rmerge(I) obs: 0.11 / Net I/σ(I): 9.9
Reflection shellResolution: 2.55→2.69 Å / Redundancy: 4 % / Rmerge(I) obs: 0.5 / Mean I/σ(I) obs: 2.4 / % possible all: 94.3

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Processing

Software
NameVersionClassification
PHENIX(PHENIX.REFINE: 1.7.2_869)refinement
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 1VQQ

1vqq


Resolution: 2.55→49.126 Å / SU ML: 0.95 / σ(F): 1.34 / Phase error: 30.78 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2904 2425 5.1 %
Rwork0.2137 --
obs0.2175 47470 93.57 %
Solvent computationShrinkage radii: 0.73 Å / VDW probe radii: 1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 31.828 Å2 / ksol: 0.338 e/Å3
Displacement parameters
Baniso -1Baniso -2Baniso -3
1--3.2747 Å20 Å20 Å2
2--13.05 Å20 Å2
3----9.7753 Å2
Refinement stepCycle: LAST / Resolution: 2.55→49.126 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms10318 0 8 216 10542
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00910501
X-RAY DIFFRACTIONf_angle_d1.47714130
X-RAY DIFFRACTIONf_dihedral_angle_d17.0084016
X-RAY DIFFRACTIONf_chiral_restr0.1461530
X-RAY DIFFRACTIONf_plane_restr0.0051816
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.55-2.60210.3631620.2752653X-RAY DIFFRACTION96
2.6021-2.65860.43021500.29732658X-RAY DIFFRACTION96
2.6586-2.72050.37471550.2912657X-RAY DIFFRACTION95
2.7205-2.78850.38321540.28542640X-RAY DIFFRACTION95
2.7885-2.86390.40071400.29082675X-RAY DIFFRACTION95
2.8639-2.94820.3521420.26822667X-RAY DIFFRACTION95
2.9482-3.04330.33741440.25312648X-RAY DIFFRACTION95
3.0433-3.15210.34731350.23832671X-RAY DIFFRACTION95
3.1521-3.27820.33911490.22232644X-RAY DIFFRACTION94
3.2782-3.42740.31021440.22832658X-RAY DIFFRACTION94
3.4274-3.6080.29231230.21772663X-RAY DIFFRACTION94
3.608-3.8340.31961150.19852669X-RAY DIFFRACTION93
3.834-4.12990.25831410.16922609X-RAY DIFFRACTION93
4.1299-4.54530.19971680.14542604X-RAY DIFFRACTION92
4.5453-5.20230.21141360.15192630X-RAY DIFFRACTION91
5.2023-6.55190.24591390.20352624X-RAY DIFFRACTION90
6.5519-49.13490.22351280.20662675X-RAY DIFFRACTION87
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.5403-0.59540.93271.9898-0.45382.40820.0751-0.4288-0.1030.336-0.0246-0.12970.3920.113-0.12310.26410.0515-0.04650.31080.03520.2098-5.2634-27.660893.3431
2-0.01380.15540.04820.42660.03390.6676-0.0832-0.0642-0.10550.05080.03050.13840.1329-0.29350.04450.1019-0.03170.01870.31890.01440.1913-25.4269-16.635564.5499
34.58780.3562-2.61251.14921.34473.60060.04010.21680.6981-0.2322-0.06140.3722-0.6352-0.6839-0.01660.17810.0806-0.04790.52550.04820.1481-34.9206-9.299354.3065
42.10510.3191-0.4840.43520.6292.20760.0321-0.4744-0.43840.591-0.1435-0.00840.8418-0.22470.03440.5689-0.09390.03460.1480.02220.1667-21.2754-26.806246.2176
50.97730.76721.34330.8362.13416.5796-0.02080.0489-0.2439-0.09810.00870.38250.13660.39460.2330.32630.078-0.03110.1328-0.0460.1734-10.7141-21.108761.6729
61.5960.01991.42430.5589-0.53362.90650.18390.19430.1902-0.11060.17210.0623-0.04490.14960.14210.10130.08820.01950.49460.07270.1341-6.5792-17.051666.1438
70.72870.22150.23550.75260.62122.293-0.08520.1922-0.0974-0.0840.01940.04650.5152-0.47940.04550.2402-0.13850.00590.2211-0.04510.1001-22.3845-24.779414.6155
81.6452-0.6594-1.13430.80710.00322.85920.10890.8151-0.8719-0.21730.18440.17660.7771-0.59210.17880.7855-0.1754-0.0670.1187-0.16740.3715-17.7009-36.677720.4901
90.3527-0.0624-0.39691.65030.22223.0776-0.02660.21710.0165-0.1132-0.0792-0.23570.49910.2404-0.02950.2204-0.0105-0.00790.1846-0.06950.1067-13.7771-22.332412.6985
101.6174-0.57920.31160.92420.23842.4189-0.0610.5932-0.0434-0.0569-0.33710.31610.1987-0.19430.12350.1827-0.1006-0.01550.31240.0086-0.6756-21.9542-16.318815.102
118.62493.98784.20273.9386-0.20714.218-0.26310.04470.5990.2121-0.13170.2196-0.0309-0.35720.24710.1258-0.04510.02980.149-0.05540.1506-22.7516-8.948716.6722
120.8049-0.50540.25991.0356-0.57920.32-0.1046-0.1610.34950.1081-0.1175-0.0792-0.12340.07210.60920.1207-0.1911-0.09130.3879-0.04740.2685-9.3177-3.170622.8338
132.74-1.15712.63394.5993-2.70443.8893-0.2454-0.68840.45240.71170.19930.5964-1.1512-0.8410.25220.52170.32470.12170.2396-0.07650.2666-33.13915.677589.0824
142.7349-0.3947-0.04511.6384-0.65692.44170.2369-0.0987-0.25740.11630.16010.3787-0.6413-0.8649-0.18910.23070.17010.03620.47880.04060.1568-32.43760.030583.3434
151.8348-0.5816-0.55462.2598-0.37563.3316-0.05560.1668-0.12320.2271-0.11180.202-0.0753-0.27540.01820.17150.01080.00780.1198-0.02480.1393-24.3989-7.752279.3668
160.966-0.2503-0.23030.5696-0.62812.00330.1596-0.2717-0.01190.2579-0.0588-0.02360.1070.0483-0.04970.169-0.0565-0.05670.31960.02240.16419.999-12.235565.2917
173.85051.599-2.1940.9241-1.40042.38690.3346-0.54130.74660.32780.22090.2042-0.60360.0312-0.1260.4042-0.0833-0.07230.56310.02490.44368.6142-5.875381.5854
182.1977-0.90222.28680.7169-1.39682.96870.22660.0325-0.3533-0.3371-0.2164-0.1730.37671.49220.08990.39690.13640.06140.73690.02230.173516.6103-17.532970.4293
191.51210.3450.32010.8158-0.65293.05910.0079-0.5260.17720.2064-0.07790.2249-0.72150.12-0.04960.2461-0.02140.05040.2415-0.10420.17647.5879-2.951857.7611
206.0361.228-4.73810.3499-0.73164.0964-0.20430.5051-0.2683-0.1998-0.0733-0.01370.1125-0.27130.01750.12540.0183-0.04050.0848-0.04010.1995-9.9883-4.983261.684
212.6699-0.1003-3.53721.85060.78594.8631-0.05330.1022-0.20220.3535-0.06340.11450.07070.33790.0715-0.00480.067-0.08340.2994-0.00190.1726-15.9813-8.990263.2335
222.14760.6669-1.05320.9224-0.40031.57910.11340.1712-0.05550.1216-0.0706-0.1519-0.31020.3461-0.02720.0060.00250.01280.3279-0.07560.119326.5084-2.436829.5033
233.35840.3532-1.31531.0485-0.3053.74430.28740.38680.1064-0.1819-0.05770.1375-0.2956-0.0852-0.09280.1280.0480.03670.2505-0.0183-0.032718.76381.066424.8466
243.8333.92691.51716.11661.1450.678-0.33660.4869-0.47610.17850.276-0.90070.47250.4359-0.63940.4986-0.0120.24070.6419-0.30970.325927.9834-15.606125.0658
254.20662.0234-1.6545.55610.99164.44990.28010.0899-0.6132-0.0648-0.0623-0.68660.34720.4167-0.31370.20870.14170.04510.2731-0.06040.164225.3325-10.820730.1068
265.4128-1.43920.91184.09311.33054.8399-0.29580.4304-0.5642-0.167-0.0888-0.30720.64780.10910.14190.26440.01440.31950.3775-0.1692-0.091418.8939-20.911431.1339
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1CHAIN A AND (RESSEQ 27:125)
2X-RAY DIFFRACTION2CHAIN A AND (RESSEQ 126:211)
3X-RAY DIFFRACTION3CHAIN A AND (RESSEQ 212:242)
4X-RAY DIFFRACTION4CHAIN A AND (RESSEQ 243:286)
5X-RAY DIFFRACTION5CHAIN A AND (RESSEQ 287:304)
6X-RAY DIFFRACTION6CHAIN A AND (RESSEQ 305:324)
7X-RAY DIFFRACTION7CHAIN A AND (RESSEQ 325:486)
8X-RAY DIFFRACTION8CHAIN A AND (RESSEQ 487:510)
9X-RAY DIFFRACTION9CHAIN A AND (RESSEQ 511:604)
10X-RAY DIFFRACTION10CHAIN A AND (RESSEQ 611:640)
11X-RAY DIFFRACTION11CHAIN A AND (RESSEQ 641:656)
12X-RAY DIFFRACTION12CHAIN A AND (RESSEQ 657:668)
13X-RAY DIFFRACTION13CHAIN B AND (RESSEQ 27:50)
14X-RAY DIFFRACTION14CHAIN B AND (RESSEQ 51:125)
15X-RAY DIFFRACTION15CHAIN B AND (RESSEQ 126:147)
16X-RAY DIFFRACTION16CHAIN B AND (RESSEQ 148:193)
17X-RAY DIFFRACTION17CHAIN B AND (RESSEQ 194:210)
18X-RAY DIFFRACTION18CHAIN B AND (RESSEQ 211:230)
19X-RAY DIFFRACTION19CHAIN B AND (RESSEQ 231:286)
20X-RAY DIFFRACTION20CHAIN B AND (RESSEQ 287:304)
21X-RAY DIFFRACTION21CHAIN B AND (RESSEQ 305:324)
22X-RAY DIFFRACTION22CHAIN B AND (RESSEQ 325:486)
23X-RAY DIFFRACTION23CHAIN B AND (RESSEQ 487:582)
24X-RAY DIFFRACTION24CHAIN B AND (RESSEQ 583:604)
25X-RAY DIFFRACTION25CHAIN B AND (RESSEQ 611:640)
26X-RAY DIFFRACTION26CHAIN B AND (RESSEQ 641:668)

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