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- PDB-3x2r: Structure of the nonameric bacterial amyloid secretion channel CsgG -
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Open data
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Basic information
Entry | Database: PDB / ID: 3x2r | ||||||
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Title | Structure of the nonameric bacterial amyloid secretion channel CsgG | ||||||
![]() | CsgG | ||||||
![]() | MEMBRANE PROTEIN / beta-barrel / outer membrane protein / curli secretion / biofim | ||||||
Function / homology | Curli production assembly/transport component CsgG / Curli production assembly/transport component CsgG / Prokaryotic membrane lipoprotein lipid attachment site profile. / outer membrane-bounded periplasmic space / Curli production assembly/transport component CsgG / CsgG![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Huang, Y. / Cao, B. / Zhao, Y. / Kou, Y. / Ni, D. / Zhang, X.C. | ||||||
![]() | ![]() Title: Structure of the nonameric bacterial amyloid secretion channel Authors: Cao, B. / Zhao, Y. / Kou, Y. / Ni, D. / Zhang, X.C. / Huang, Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 661.6 KB | Display | ![]() |
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PDB format | ![]() | 548.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 510.8 KB | Display | ![]() |
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Full document | ![]() | 563.3 KB | Display | |
Data in XML | ![]() | 61.3 KB | Display | |
Data in CIF | ![]() | 81.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 30584.035 Da / Num. of mol.: 9 / Fragment: Chymotrypsin digest fragment / Mutation: none Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.86 Å3/Da / Density % sol: 56.93 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 50mM Tris-HCl(pH8.5), 300mM MgCl2, 14% PEG2000, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Mar 13, 2014 |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9792 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→50 Å / Num. all: 61340 / Num. obs: 61340 / % possible obs: 88.2 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 |
Reflection shell | Resolution: 2.9→3 Å / % possible all: 86.9 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.8 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.9→48.071 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 22
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Refinement TLS params. | Method: refined / Origin x: 206.9173 Å / Origin y: 36.5217 Å / Origin z: 5.7516 Å
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Refinement TLS group | Selection details: all |