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- PDB-3wuu: Structure basis of inactivating cell abscission with chimera peptide 1 -
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Open data
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Basic information
Entry | Database: PDB / ID: 3wuu | ||||||
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Title | Structure basis of inactivating cell abscission with chimera peptide 1 | ||||||
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![]() | CELL CYCLE / Coiled-coil | ||||||
Function / homology | ![]() intercellular bridge organization / negative regulation of cytokinesis / mitotic sister chromatid separation / attachment of spindle microtubules to kinetochore / male meiotic nuclear division / cranial skeletal system development / midbody abscission / Flemming body / intercellular bridge / mitotic spindle assembly checkpoint signaling ...intercellular bridge organization / negative regulation of cytokinesis / mitotic sister chromatid separation / attachment of spindle microtubules to kinetochore / male meiotic nuclear division / cranial skeletal system development / midbody abscission / Flemming body / intercellular bridge / mitotic spindle assembly checkpoint signaling / regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / cleavage furrow / mitotic cytokinesis / centriole / cellular response to leukemia inhibitory factor / establishment of protein localization / kinetochore / midbody / protein kinase activity / cell division / centrosome / protein kinase binding / extracellular exosome / ATP binding / identical protein binding / membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kim, H.J. / Matsuura, A. / Lee, H.H. | ||||||
![]() | ![]() Title: Structural and biochemical insights into the role of testis-expressed gene 14 (TEX14) in forming the stable intercellular bridges of germ cells. Authors: Kim, H.J. / Yoon, J. / Matsuura, A. / Na, J.H. / Lee, W.K. / Kim, H. / Choi, J.W. / Park, J.E. / Park, S.J. / Kim, K.T. / Chang, R. / Lee, B.I. / Yu, Y.G. / Shin, Y.K. / Jeong, C. / Rhee, K. / Lee, H.H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 103.8 KB | Display | ![]() |
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PDB format | ![]() | 82.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 523.9 KB | Display | ![]() |
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Full document | ![]() | 536.3 KB | Display | |
Data in XML | ![]() | 18.9 KB | Display | |
Data in CIF | ![]() | 26.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 7243.210 Da / Num. of mol.: 8 / Fragment: UNP residues 160-217 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Protein/peptide | Mass: 1462.603 Da / Num. of mol.: 4 / Mutation: P791D/I802P/P803G/P804Y / Source method: obtained synthetically / Details: synthetic peptide / Source: (synth.) ![]() #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.88 Å3/Da / Density % sol: 57.33 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 20%(w/v) polyacrylic acid 5100, 0.2M magnesium chloride, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Apr 23, 2014 |
Radiation | Monochromator: si 111 DCM / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9781 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→50 Å / Num. all: 17398 / Num. obs: 17000 / % possible obs: 99.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Biso Wilson estimate: 62.34 Å2 |
Reflection shell | Highest resolution: 2.9 Å / % possible all: 99.8 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 116.08 Å2 / Biso mean: 48.88 Å2 / Biso min: 12.01 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.904→49.374 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 12
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