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Yorodumi- PDB-3uzo: Crystal Structures of Branched-Chain Aminotransferase from Deinoc... -
+Open data
-Basic information
Entry | Database: PDB / ID: 3uzo | ||||||
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Title | Crystal Structures of Branched-Chain Aminotransferase from Deinococcus radiodurans Complexes with alpha-Ketoisocaproate and L-Glutamate Suggest Its Radio-Resistance for Catalysis | ||||||
Components | Branched-chain-amino-acid aminotransferase | ||||||
Keywords | TRANSFERASE / BCAT / Amino-acid biosynthesis / Aminotransferase / Branched-chain amino acid biosynthesis / Pyridoxal phosphate / L-Glutamate | ||||||
Function / homology | Function and homology information L-leucine:2-oxoglutarate aminotransferase activity / branched-chain-amino-acid transaminase / L-leucine transaminase activity / L-valine transaminase activity / L-isoleucine transaminase activity / branched-chain-amino-acid transaminase activity / L-leucine biosynthetic process / valine biosynthetic process / isoleucine biosynthetic process / amino acid biosynthetic process Similarity search - Function | ||||||
Biological species | Deinococcus radiodurans (radioresistant) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | Chen, C.D. / Huang, Y.C. / Chuankhayan, P. / Hsieh, Y.C. / Huang, T.F. / Lin, C.H. / Guan, H.H. / Liu, M.Y. / Chang, W.C. / Chen, C.J. | ||||||
Citation | Journal: J.Bacteriol. / Year: 2012 Title: Crystal Structures of Complexes of the Branched-Chain Aminotransferase from Deinococcus radiodurans with alpha-Ketoisocaproate and L-Glutamate Suggest the Radiation Resistance of This Enzyme for Catalysis Authors: Chen, C.D. / Lin, C.H. / Chuankhayan, P. / Huang, Y.C. / Hsieh, Y.C. / Huang, T.F. / Guan, H.H. / Liu, M.Y. / Chang, W.C. / Chen, C.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3uzo.cif.gz | 142.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3uzo.ent.gz | 112.5 KB | Display | PDB format |
PDBx/mmJSON format | 3uzo.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 3uzo_validation.pdf.gz | 473.3 KB | Display | wwPDB validaton report |
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Full document | 3uzo_full_validation.pdf.gz | 497.1 KB | Display | |
Data in XML | 3uzo_validation.xml.gz | 29.9 KB | Display | |
Data in CIF | 3uzo_validation.cif.gz | 40.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/uz/3uzo ftp://data.pdbj.org/pub/pdb/validation_reports/uz/3uzo | HTTPS FTP |
-Related structure data
Related structure data | 3uyyC 3uzbC 2cojS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 39465.324 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Deinococcus radiodurans (radioresistant) Strain: R1 / Gene: DR_1626 / Plasmid: pQE30 / Production host: Escherichia coli (E. coli) References: UniProt: Q9RTX5, branched-chain-amino-acid transaminase #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.05 Å3/Da / Density % sol: 39.88 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.4 Details: 0.2M Na Chloride, 25%(w/v) polyethylene glycol 3350, 0.1M Bis-Tris, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.4 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSRRC / Beamline: BL13B1 / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Mar 24, 2008 |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2→30 Å / Num. all: 53244 / Num. obs: 42767 / % possible obs: 99.4 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 4.9 % / Biso Wilson estimate: 25.09 Å2 / Rmerge(I) obs: 0.042 / Rsym value: 0.06 / Net I/σ(I): 26.7 |
Reflection shell | Resolution: 2→2.09 Å / Redundancy: 4.8 % / Rmerge(I) obs: 0.19 / Mean I/σ(I) obs: 8.61 / Num. unique all: 5291 / Rsym value: 0.201 / % possible all: 99.2 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB 2COJ Resolution: 2→30 Å / Cross valid method: THROUGHOUT / σ(F): 2 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 24.9072 Å2
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Refinement step | Cycle: LAST / Resolution: 2→30 Å
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LS refinement shell | Resolution: 2→2.09 Å / Rfactor Rfree error: 0.201
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