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Open data
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Basic information
Entry | Database: PDB / ID: 3uy5 | ||||||
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Title | crystal structure of Eis from Mycobacterium tuberculosis | ||||||
![]() | Enhanced intracellular survival protein | ||||||
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Function / homology | ![]() modulation by symbiont of host programmed cell death / effector-mediated defense to host-produced reactive oxygen species / modulation by symbiont of host inflammatory response / suppression by symbiont of host defense-related programmed cell death / modulation by symbiont of host innate immune response / aminoglycoside N-acetyltransferase activity / aminoglycoside antibiotic catabolic process / bacterial extracellular vesicle / Suppression of autophagy / host extracellular space ...modulation by symbiont of host programmed cell death / effector-mediated defense to host-produced reactive oxygen species / modulation by symbiont of host inflammatory response / suppression by symbiont of host defense-related programmed cell death / modulation by symbiont of host innate immune response / aminoglycoside N-acetyltransferase activity / aminoglycoside antibiotic catabolic process / bacterial extracellular vesicle / Suppression of autophagy / host extracellular space / biological process involved in interaction with host / host cell cytoplasmic vesicle / ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kim, K.H. / Suh, S.W. | ||||||
![]() | ![]() Title: Mycobacterium tuberculosis Eis protein initiates suppression of host immune responses by acetylation of DUSP16/MKP-7 Authors: Kim, K.H. / An, D.R. / Song, J. / Yoon, J.Y. / Kim, H.S. / Yoon, H.J. / Im, H.N. / Kim, J. / Kim, D.J. / Lee, S.J. / Kim, K.H. / Lee, H.M. / Kim, H.J. / Jo, E.K. / Lee, J.Y. / Suh, S.W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 87.4 KB | Display | ![]() |
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PDB format | ![]() | 70.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 288 KB | Display | ![]() |
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Full document | ![]() | 296.2 KB | Display | |
Data in XML | ![]() | 18.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 46644.797 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.86 Å3/Da / Density % sol: 68.14 % |
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Crystal grow![]() | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 7 Details: 300mM ammonium citrate, 12% PEG 3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.46→20 Å / Num. obs: 26334 |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Shrinkage radii: 0.95 Å / VDW probe radii: 1.2 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 40 Å2 / ksol: 0.409 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 110.82 Å2 / Biso mean: 32.7382 Å2 / Biso min: 12.74 Å2
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Refinement step | Cycle: LAST / Resolution: 2.5→19.841 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 9
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