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Open data
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Basic information
Entry | Database: PDB / ID: 3uu2 | ||||||
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Title | Salmonella typhi osmoporin(OmpC):an Outer Membrane Protein | ||||||
![]() | Outer membrane protein C | ||||||
![]() | MEMBRANE PROTEIN / Beta Barrel / Non specific porin / osmoporin / Outer Membrane | ||||||
Function / homology | ![]() porin activity / pore complex / monoatomic ion transmembrane transport / cell outer membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Prasanth, P. / Putcha, B.K. / Arockiasamy, A. / Krishnaswamy, S. | ||||||
![]() | ![]() Title: Crystal Structure of Outer membrane protein OmpC from Salmonella typhi. Authors: Prasanth, P. / Putcha, B.K. / Arockiasamy, A. / Krishnaswamy, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 371.6 KB | Display | ![]() |
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PDB format | ![]() | 311.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 450.7 KB | Display | ![]() |
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Full document | ![]() | 509.7 KB | Display | |
Data in XML | ![]() | 39.3 KB | Display | |
Data in CIF | ![]() | 52.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3upgS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 39207.059 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: Ty21a / Gene: ompC, STM2267 / Plasmid: pET21b+ / Production host: ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.22 Å3/Da / Density % sol: 70.83 % |
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Crystal grow | Temperature: 298.15 K / Method: microbatch under oil / pH: 8.5 Details: 0.01M Nickel(II) chloride hexahydrate, 0.1M Tris, 20% PEG MME 2000, pH 8.5, Microbatch under oil, temperature 298.15K |
-Data collection
Diffraction | Mean temperature: 77.03 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: May 4, 2010 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0048 Å / Relative weight: 1 |
Reflection | Resolution: 3.33→100.27 Å / Num. all: 28544 / Num. obs: 27261 / % possible obs: 95.7 % / Redundancy: 12.1 % / Biso Wilson estimate: 97.8 Å2 / Rmerge(I) obs: 0.11 / Net I/σ(I): 23 |
Reflection shell | Resolution: 3.59→3.67 Å / Redundancy: 9.7 % / Rmerge(I) obs: 0.526 / Mean I/σ(I) obs: 2.95 / % possible all: 93.2 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3UPG Resolution: 3.59→50 Å / Cor.coef. Fo:Fc: 0.876 / Cor.coef. Fo:Fc free: 0.848 / SU B: 130.247 / SU ML: 0.857 / Isotropic thermal model: Overall / Cross valid method: THROUGHOUT / ESU R Free: 0.748 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 225.198 Å2
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Refinement step | Cycle: LAST / Resolution: 3.59→50 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.59→3.683 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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