THE TWO DESCRIBED BIOMOLECULES 1 AND 2 ARE RELATED BY NON-CRYSTALLOGRAPHIC TWOFOLD SYMMETRY. IT IS PLAUSIBLE THAT TOGETHER THEY FORM A FUNCTIONAL TETRAMER (WHICH MAY ALSO BE DESCRIBED AS A DIMER OF HETERO-DIMERS, BIOMOLECULE 3).
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Components
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Hydrogenase-1 ... , 2 types, 4 molecules STLM
#1: Protein
Hydrogenase-1smallchain
Mass: 36814.676 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: MC4100 / Gene: b0972, hyaA, JW0954 / Production host: Escherichia coli (E. coli) / Strain (production host): FTH004 / References: UniProt: P69739, hydrogenase (acceptor)
#2: Protein
Hydrogenase-1largechain
Mass: 64751.387 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: MC4100 / Gene: b0973, hyaB, JW0955 / Production host: Escherichia coli (E. coli) / Strain (production host): FTH004 / References: UniProt: P0ACD8, hydrogenase (acceptor)
Mass: 18.015 Da / Num. of mol.: 1257 / Source method: isolated from a natural source / Formula: H2O
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Details
Nonpolymer details
SF3 DESIGNATES A FE4-S3 CLUSTER IN A VERY OXIDIZED STATE WITH TOTAL CORE CHARGE OF +5 F4S ...SF3 DESIGNATES A FE4-S3 CLUSTER IN A VERY OXIDIZED STATE WITH TOTAL CORE CHARGE OF +5 F4S DESIGNATES THE SAME FE4-S3 CLUSTER IN A MORE REDUCED STATE WITH TOTAL CORE CHARGE OF EITHER +4 OR +3 RELATIONSHIP BETWEEN ATOM NUMBERS IN SF3 AND F4S: FS4 ATOMS FE1, FE2, FE3, FE4, S1, S2 AND S3 CORRESPOND TO SF3 ATOMS FE4, FE1, FE3, FE7, S2, S3 AND S1, RESPECTIVELY
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.07 Å3/Da / Density % sol: 40.64 %
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