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Structure paper

TitleX-ray crystallographic and computational studies of the O2-tolerant [NiFe]-hydrogenase 1 from Escherichia coli.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 109, Page 5305-5310, Year 2012
Publish dateNov 21, 2011 (structure data deposition date)
AuthorsVolbeda, A. / Amara, P. / Darnault, C. / Mouesca, J.M. / Parkin, A. / Roessler, M.M. / Armstrong, F.A. / Fontecilla-Camps, J.C.
External linksProc. Natl. Acad. Sci. USA / PubMed:22431599
MethodsX-ray diffraction
Resolution1.47 - 2 Å
Structure data

PDB-3uqy:
H2-reduced structure of E. coli hydrogenase-1
Method: X-RAY DIFFRACTION / Resolution: 1.47 Å

PDB-3usc:
Crystal Structure of E. coli hydrogenase-1 in a ferricyanide-oxidized form
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-3use:
Crystal Structure of E. coli hydrogenase-1 in its as-isolated form
Method: X-RAY DIFFRACTION / Resolution: 1.67 Å

Chemicals

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-F3S:
FE3-S4 CLUSTER

ChemComp-F4S:
FE4-S3 CLUSTER

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

ChemComp-CL:
Unknown entry

ChemComp-SO4:
SULFATE ION

ChemComp-FCO:
CARBONMONOXIDE-(DICYANO) IRON

ChemComp-NI:
NICKEL (II) ION

ChemComp-MG:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-SF3:
FE4-S3 CLUSTER

ChemComp-3NI:
NICKEL (III) ION

ChemComp-LI:
Unknown entry

Source
  • escherichia coli (E. coli)
KeywordsOXIDOREDUCTASE / MEMBRANE-BOUND HYDROGENASE

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