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- PDB-3ukf: CRYSTAL STRUCTURE OF UDP-galactopyranose mutase from Aspergillus ... -

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Basic information

Entry
Database: PDB / ID: 3ukf
TitleCRYSTAL STRUCTURE OF UDP-galactopyranose mutase from Aspergillus fumigatus in complex with UDPgalp (reduced)
ComponentsUDP-galactopyranose mutase
KeywordsISOMERASE / FLAVOENZYME / FADH2
Function / homology
Function and homology information


UDP-galactopyranose mutase / UDP-galactopyranose mutase activity / cell wall organization / nucleotide binding
Similarity search - Function
NAD(P)-binding Rossmann-like domain / FAD/NAD(P)-binding domain / FAD/NAD(P)-binding domain / 3-Layer(bba) Sandwich / FAD/NAD(P)-binding domain superfamily / Alpha Beta
Similarity search - Domain/homology
DIHYDROFLAVINE-ADENINE DINUCLEOTIDE / GALACTOSE-URIDINE-5'-DIPHOSPHATE / UDP-galactopyranose mutase
Similarity search - Component
Biological speciesAspergillus fumigatus (mold)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.5 Å
AuthorsVan Straaten, K.E. / Sanders, D.A.R.
CitationJournal: J.Biol.Chem. / Year: 2012
Title: Structural Insight into the Unique Substrate Binding Mechanism and Flavin Redox State of UDP-galactopyranose Mutase from Aspergillus fumigatus.
Authors: van Straaten, K.E. / Routier, F.H. / Sanders, D.A.
History
DepositionNov 9, 2011Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 22, 2012Provider: repository / Type: Initial release
Revision 1.1Mar 7, 2012Group: Database references
Revision 1.2Jul 18, 2012Group: Database references
Revision 1.3Nov 8, 2017Group: Refinement description / Category: software / Item: _software.name
Revision 1.4Nov 20, 2024Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description / Structure summary
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_entry_details / pdbx_initial_refinement_model / pdbx_modification_feature / refine / struct_conn / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _refine.pdbx_starting_model / _struct_conn.pdbx_leaving_atom_flag / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: UDP-galactopyranose mutase
B: UDP-galactopyranose mutase
C: UDP-galactopyranose mutase
D: UDP-galactopyranose mutase
E: UDP-galactopyranose mutase
F: UDP-galactopyranose mutase
G: UDP-galactopyranose mutase
H: UDP-galactopyranose mutase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)470,99232
Polymers459,8778
Non-polymers11,11524
Water13,619756
1
A: UDP-galactopyranose mutase
H: UDP-galactopyranose mutase
hetero molecules

C: UDP-galactopyranose mutase
F: UDP-galactopyranose mutase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)235,49616
Polymers229,9394
Non-polymers5,55712
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation1_545x,y-1,z1
Buried area19200 Å2
ΔGint-151 kcal/mol
Surface area77880 Å2
MethodPISA
2
B: UDP-galactopyranose mutase
D: UDP-galactopyranose mutase
E: UDP-galactopyranose mutase
G: UDP-galactopyranose mutase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)235,49616
Polymers229,9394
Non-polymers5,55712
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area19200 Å2
ΔGint-151 kcal/mol
Surface area77900 Å2
MethodPISA
Unit cell
Length a, b, c (Å)72.083, 129.338, 175.077
Angle α, β, γ (deg.)89.97, 103.61, 90.14
Int Tables number1
Space group name H-MP1
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-ID
11
21
31
41
51
61
71
81

NCS domain segments:
Dom-IDComponent-IDEns-IDSelection details
111chain A and (resseq 2:353 or resseq 365:500 ) and (not element H) and (not element D)
211chain B and (resseq 2:353 or resseq 365:500 ) and (not element H) and (not element D)
311chain C and (resseq 2:353 or resseq 365:500 ) and (not element H) and (not element D)
411chain D and (resseq 2:353 or resseq 365:500 ) and (not element H) and (not element D)
511chain E and (resseq 2:353 or resseq 365:500 ) and (not element H) and (not element D)
611chain F and (resseq 2:353 or resseq 365:500 ) and (not element H) and (not element D)
711chain G and (resseq 2:353 or resseq 365:500 ) and (not element H) and (not element D)
811chain H and (resseq 2:353 or resseq 365:500 ) and (not element H) and (not element D)

NCS oper:
IDCodeMatrixVector
1given(-0.999995, -0.00311, -0.000747), (-0.003109, 0.999995, -0.000859), (0.00075, -0.000857, -0.999999)-9.85739, -64.668297, 36.394699
2given(-0.880785, 0.029741, -0.472581), (0.146501, -0.931942, -0.331696), (-0.450283, -0.361386, 0.816484)24.8929, 53.872299, 41.362099
3given(0.880247, 0.030908, 0.473509), (-0.145335, -0.932359, 0.331035), (0.451712, -0.36021, -0.816214)18.4702, -19.7773, 52.2155
4given(0.999568, -0.028931, -0.005092), (0.025149, 0.932361, -0.360652), (0.015181, 0.360368, 0.932687)51.619999, -40.697498, -86.448898
5given(0.889568, -0.000676, 0.456801), (0.000219, -0.999998, -0.001908), (0.456802, 0.001797, -0.889567)11.7923, 70.328796, -48.769699
6given(-0.888607, -0.001697, -0.458666), (0.001147, -0.999998, 0.001476), (-0.458668, 0.000786, 0.888607)19.495701, 5.6491, -85.555298
7given(-0.999493, -0.031168, 0.006451), (-0.026753, 0.932478, 0.360234), (-0.017243, 0.359879, -0.93284)39.458099, 6.36767, -29.373301

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Components

#1: Protein
UDP-galactopyranose mutase


Mass: 57484.684 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Aspergillus fumigatus (mold) / Strain: CBS 144-89 / Gene: glf, glfA / Plasmid: pET22b / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) GOLD / References: UniProt: Q4W1X2, UDP-galactopyranose mutase
#2: Chemical
ChemComp-GDU / GALACTOSE-URIDINE-5'-DIPHOSPHATE / UDP-D-GALACTOPYRANOSE


Mass: 566.302 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Formula: C15H24N2O17P2
#3: Chemical
ChemComp-FDA / DIHYDROFLAVINE-ADENINE DINUCLEOTIDE


Mass: 787.566 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: C27H35N9O15P2
#4: Chemical
ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: Cl
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 756 / Source method: isolated from a natural source / Formula: H2O
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.45 Å3/Da / Density % sol: 64.34 %
Crystal growTemperature: 295 K / Method: microbatch / pH: 8.5
Details: 0.1M Bis-Tris-Propane pH 8.5, 0.2-0.25M sodium citrate, 15-20% PEG 3350, microbatch, temperature 295K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: CLSI / Beamline: 08ID-1 / Wavelength: 0.9809 Å
DetectorType: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Dec 12, 2008
RadiationMonochromator: double crystal monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9809 Å / Relative weight: 1
ReflectionResolution: 2.4→20 Å / Num. obs: 233082 / % possible obs: 97.2 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Redundancy: 3.56 % / Rmerge(I) obs: 0.114 / Rsym value: 0.114 / Net I/σ(I): 5.6
Reflection shell
Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsDiffraction-ID% possible all
2.4-2.493.520.6461.6192.2
2.49-2.583.690.5891.8197.9
2.58-2.73.690.5212.2198
2.7-2.843.660.4242.7198.1
2.84-3.023.660.3223.5198.3
3.02-3.253.60.2334.7198.4
3.25-3.583.550.1656.3198.3
3.58-4.093.470.1168.6198.1
4.09-5.143.370.08911.9197.1
5.14-203.350.07913.8195.3

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Processing

Software
NameVersionClassificationNB
d*TREKdata scaling
d*TREK9.9.8.0Ldata reduction
PHENIX1.7.1_743refinement
PDB_EXTRACT3.1data extraction
MxDCdata collection
MOLREPphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.5→20 Å / Occupancy max: 1 / Occupancy min: 1 / SU ML: 1.23 / Isotropic thermal model: Isotropic / σ(F): 1.99 / Phase error: 35.18 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2935 10347 4.99 %
Rwork0.2499 --
obs0.2521 207280 97.67 %
Solvent computationShrinkage radii: 0.83 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 63.439 Å2 / ksol: 0.364 e/Å3
Displacement parameters
Baniso -1Baniso -2Baniso -3
1-20.7697 Å2-1.9475 Å218.1365 Å2
2---13.9192 Å21.0949 Å2
3----11.1208 Å2
Refinement stepCycle: LAST / Resolution: 2.5→20 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms31968 0 720 756 33444
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00333528
X-RAY DIFFRACTIONf_angle_d0.6445696
X-RAY DIFFRACTIONf_dihedral_angle_d17.32212704
X-RAY DIFFRACTIONf_chiral_restr0.0474952
X-RAY DIFFRACTIONf_plane_restr0.0075832
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDTypeRms dev position (Å)
11A3839X-RAY DIFFRACTIONPOSITIONAL
12B3839X-RAY DIFFRACTIONPOSITIONAL0.025
13C3839X-RAY DIFFRACTIONPOSITIONAL0.04
14D3839X-RAY DIFFRACTIONPOSITIONAL0.041
15E3839X-RAY DIFFRACTIONPOSITIONAL0.039
16F3839X-RAY DIFFRACTIONPOSITIONAL0.033
17G3839X-RAY DIFFRACTIONPOSITIONAL0.033
18H3839X-RAY DIFFRACTIONPOSITIONAL0.04
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.5-2.58920.499810420.452719746X-RAY DIFFRACTION98
2.5892-2.69260.445210540.403819803X-RAY DIFFRACTION98
2.6926-2.81480.42029930.379119748X-RAY DIFFRACTION98
2.8148-2.96280.373310080.326619857X-RAY DIFFRACTION98
2.9628-3.14780.343510670.291119826X-RAY DIFFRACTION98
3.1478-3.38970.317810430.253419785X-RAY DIFFRACTION98
3.3897-3.72890.287810220.235819797X-RAY DIFFRACTION98
3.7289-4.26390.267210390.225119747X-RAY DIFFRACTION98
4.2639-5.35490.223410350.189319487X-RAY DIFFRACTION97
5.3549-20.00050.254410440.217519137X-RAY DIFFRACTION95
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.00980.0001-0.0535-0.0088-0.01040.0697-0.05020.04990.05890.0595-0.01110.01460.01470.1427-0.0938-0.04370.0377-0.08370.41930.01260.29883.513613.70042.167
20.3062-0.00920.1830.01590.00930.1123-0.13170.05010.0083-0.1225-0.0648-0.02740.0640.0591-0.15490.5984-0.05130.1550.2291-0.03980.27814.2839-21.0141-4.3047
3-0.0013-0.00070.00190.00760.00770.0046-0.09020.0107-0.01960.01870.0196-0.0443-0.02030.032900.4750.11110.07020.3471-0.01240.360614.5279-22.7971.4072
40.02660.0366-0.03480.0502-0.01470.0644-0.0594-0.0160.16810.04470.0701-0.04510.0822-0.01790.01680.0586-0.0179-0.09840.38430.05590.2704-2.292314.339-6.1386
50.13720.0239-0.02050.1003-0.07720.1575-0.1479-0.04280.0061-0.05910.085-0.04570.25710.0263-0.1520.0754-0.0213-0.03340.3253-0.02560.2357-6.3348-2.80874.3576
60.0326-0.0027-0.02660.005-0.00060.0254-0.03590.0281-0.0177-0.0268-0.0360.06240.01450.0011-0.11320.13110.0652-0.1790.4005-0.01750.293319.26412.6178-6.7417
70.01590.0103-0.03560.01440.02410.0396-0.08290.06180.0599-0.0839-0.062-0.01270.0116-0.1033-0.227-0.1196-0.0596-0.13890.3364-0.02020.1944-13.641178.358634.1477
80.01380.0323-0.00930.0258-0.01790.0029-0.11370.08170.0690.0818-0.00410.08620.1831-0.0784-0.00240.58160.01350.10350.28070.01770.2961-14.308943.645640.6373
9-0.00120.002-0.00260.0051-0.0050.0032-0.0982-0.01130.0043-0.02880.02390.0677-0.0516-0.04830.00010.5872-0.08660.06110.4191-0.02430.3709-24.049945.202433.791
100.0298-0.0283-0.010.06150.02460.008-0.07340.04990.11560.0040.10450.0907-0.03170.01470.0626-0.01780.0192-0.16360.3241-0.07510.2015-5.087478.296443.3434
110.0051-0.0285-0.02910.04880.01180.0559-0.04740.2131-0.0171-0.02330.0569-0.04690.10840.03460.01650.1771-0.0092-0.02950.36470.00050.288-0.499957.808721.0755
120.11930.00960.06810.0092-0.01010.1269-0.1144-0.0146-0.11-0.05680.0313-0.01590.1885-0.0005-0.099-0.07740.0789-0.15440.3639-0.01210.2522-13.815468.980541.463
130.0113-0.03730.00690.0629-0.01460.08570.21010.03-0.032-0.1601-0.0211-0.09760.1118-0.06280.1330.26730.0062-0.01940.3963-0.01050.342330.251350.985-8.2382
140.0136-0.02-0.01140.0368-0.02060.08890.0720.07440.0289-0.1258-0.0432-0.0795-0.1505-0.1993-0.0020.5559-0.05190.18330.255-0.04670.452827.586485.3487-2.6537
150.0020.0048-0.00160.0076-0.00410.004-0.0378-0.0003-0.0401-0.00550.00980.0098-0.0423-0.000800.7376-0.01360.24560.5229-0.01260.524716.316381.9399-2.2641
160.0309-0.01780.00230.0778-0.06910.11210.102-0.10110.0609-0.0888-0.0064-0.21140.00150.03840.01270.33820.02320.09640.3433-0.06560.453542.273654.0274-12.0313
170.00560.00990.01460.02350.01140.07850.0865-0.0401-0.084-0.01210.04610.1164-0.0595-0.11020.04320.2742-0.0988-0.03320.4304-0.0480.360330.520163.849717.2425
180.1531-0.0343-0.13270.2212-0.01610.10240.18720.00770.2035-0.1302-0.0055-0.0537-0.00330.00560.0830.3292-0.03460.03070.2768-0.05630.306933.636563.1061-7.931
190.02210.04970.02760.17110.02420.04880.1892-0.011-0.00840.1616-0.03780.0790.0832-0.04940.09260.17820.0075-0.02870.29020.02160.2337-40.3833-13.622644.6474
200.01140.009-0.01760.01690.03440.66590.0878-0.01030.06180.10880.03440.0593-0.11720.26560.01710.47640.04990.13320.20710.05190.4273-37.785520.622239.188
21-0.00160.00140.00220.00370.00390.0072-0.0590.03410.01110.0256-0.0545-0.0447-0.0181-0.0199-00.6687-0.02850.1810.5048-0.00860.4876-25.3618.408937.782
220.01730.00650.00570.06880.06240.06390.06590.0510.04010.12220.00690.23530.1138-0.04540.0010.3847-0.03840.09420.36130.05340.4602-52.4324-10.678848.5963
230.0066-0.0034-0.02290.0253-0.01850.03870.04040.08570.09070.03720.0484-0.0347-0.05110.12540.01590.25480.0571-0.00560.38590.0590.399-40.5038-1.180219.1656
240.11660.0331-0.09310.20860.00690.09320.21060.04070.16440.137-0.02160.0728-0.02460.02610.06070.31080.01870.04450.27040.06480.3078-43.9874-1.356646.1355
250.4614-0.25660.29640.42070.00260.2847-0.11920.22270.15470.3784-0.08230.22610.09970.1218-0.25980.25510.03410.00270.246-0.05060.2367-45.393884.037363.2447
260.08340.0202-0.02280.0274-0.04560.0963-0.0263-0.01090.0060.14870.01260.11090.02070.0733-0.0011.157-0.05170.60390.26010.09870.5713-45.593349.324469.7251
27-0.00060.00230.00270.0140.01110.0083-0.07190.03090.04720.0181-0.07140.0144-0.0153-0.027-0.00031.00930.04710.19460.41070.08910.4027-35.319649.400975.6579
280.2619-0.09820.02220.2160.27460.584-0.2218-0.0645-0.14020.2878-0.14680.36270.1370.0016-0.32290.2393-0.0636-0.02870.3914-0.02180.4644-51.294881.796555.2892
290.04440.03440.11010.2071-0.01410.3249-0.2812-0.1704-0.28350.9008-0.02460.64450.26340.0221-0.57380.7271-0.17530.7250.1540.13220.7359-55.618169.732171.2788
300.0395-0.019-0.03540.04920.020.0352-0.0876-0.0159-0.00210.03150.05640.08360.024-0.0165-0.07510.1713-0.0143-0.10210.3770.00050.3129-29.844779.410755.2438
310.0991-0.06310.06630.0656-0.03050.03290.1742-0.0457-0.0524-0.0986-0.01290.0090.0836-0.02360.13461.1260.00580.00830.41920.02230.224415.892456.6213-49.002
320.01080.0108-0.02350.04230.00750.01720.07860.01060.004-0.02330.004-0.0395-0.04670.03220.26741.21170.0060.37940.22730.12270.011713.610691.3225-42.8313
33-0.00020.0001-0.00320.00450.00460.01390.0155-0.0290.0386-0.00370.0509-0.06530.01120.0062-01.1796-0.06490.23860.5399-0.04180.43925.400589.7434-44.4676
340.0848-0.03870.05440.0411-0.0050.06220.16460.0083-0.0239-0.16320.0614-0.03280.0712-0.04430.15431.0736-0.0822-0.03910.42130.06690.19744.072356.7078-44.7309
350.00650.0015-0.03140.0108-0.00020.09010.00510.10330.0582-0.00840.0615-0.0451-0.03820.03540.23921.23840.10680.20460.36390.13870.061510.229277.2043-66.5461
360.4023-0.01050.20230.01910.03340.37160.217-0.0745-0.0177-0.16860.0135-0.1518-0.04380.04290.3330.9040.01460.11350.20490.06260.098512.728765.9876-42.3783
370.09490.06470.08410.05870.01740.09260.21260.1494-0.07840.109-0.0226-0.03260.0908-0.01540.29331.0141-0.1018-0.02910.3678-0.0390.0962-26.3407-7.777885.5968
380.1022-0.0275-0.01720.10930.09640.10140.1280.02260.05950.0309-0.03140.1056-0.054-0.0420.25771.3442-0.10540.31810.3308-0.05760.128-23.976426.483779.2956
390.00130.0023-0.00490.0052-0.00780.01420.00940.02050.0110.02490.04410.0358-0.00810.0246-01.17250.07150.28520.52980.080.5224-35.580525.053180.7642
400.05770.04930.01390.04580.00020.05260.0842-0.0058-0.0030.17940.0330.02950.0193-0.03160.03781.0963-0.0426-0.0380.4182-0.01980.268-14.1963-7.955181.0506
410.0127-0.00910.01460.0252-0.07870.3111-0.0498-0.05460.0061-0.12890.0270.11820.0532-0.10150.12431.3313-0.18870.17240.466-0.09850.1544-23.068212.662105.1987
420.2630.11820.12920.0490.0610.2110.26190.0150.02260.25350.00610.0771-0.2178-0.01260.5950.9907-0.09320.12190.2715-0.11760.082-21.34633.123481.7731
430.77090.12770.35660.5431-0.19510.2901-0.1447-0.14560.1399-0.264-0.0741-0.22490.0579-0.0534-0.44590.1879-0.01950.00640.15650.02520.148435.068519.1785-27.2988
440.0461-0.0116-0.00640.04810.0590.0862-0.03390.01930.0825-0.0960.0505-0.1436-0.0796-0.0543-0.07321.11280.00020.49370.1947-0.09710.50935.354-15.0696-33.3291
450.00680.0001-0.00160.0079-0.00460.0027-0.0343-0.02110.0479-0.0253-0.0193-0.00460.01370.0211-0.00111.0909-0.10630.22870.4884-0.07010.359725.0376-12.8573-40.3898
460.13430.09610.05870.3094-0.07130.4196-0.15790.0204-0.2389-0.2813-0.1559-0.38950.09760.0391-0.35540.16670.08920.01560.27010.09280.487843.85716.2455-18.1593
470.2521-0.00020.05670.1512-0.26260.4959-0.10920.1711-0.1376-0.035-0.0820.044-0.00830.0271-0.34281.3495-0.12770.62470.4273-0.04710.663747.12926.7019-49.7375
480.32150.12050.07970.7234-0.18950.4761-0.1720.0301-0.1195-0.6126-0.0428-0.50010.1406-0.139-0.26110.17810.05620.16610.25910.02570.522737.49716.9198-24.2646
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1(chain A and resid 2:90)
2X-RAY DIFFRACTION2(chain A and resid 91:180)
3X-RAY DIFFRACTION3(chain A and resid 181:199)
4X-RAY DIFFRACTION4(chain A and resid 200:284)
5X-RAY DIFFRACTION5(chain A and resid 285:474)
6X-RAY DIFFRACTION6(chain A and resid 475:511)
7X-RAY DIFFRACTION7(chain B and resid 2:90)
8X-RAY DIFFRACTION8(chain B and resid 91:180)
9X-RAY DIFFRACTION9(chain B and resid 181:208)
10X-RAY DIFFRACTION10(chain B and resid 209:302)
11X-RAY DIFFRACTION11(chain B and resid 303:387)
12X-RAY DIFFRACTION12(chain B and resid 388:511)
13X-RAY DIFFRACTION13(chain C and resid 4:89)
14X-RAY DIFFRACTION14(chain C and resid 90:180)
15X-RAY DIFFRACTION15(chain C and resid 181:208)
16X-RAY DIFFRACTION16(chain C and resid 209:302)
17X-RAY DIFFRACTION17(chain C and resid 303:363)
18X-RAY DIFFRACTION18(chain C and resid 364:511)
19X-RAY DIFFRACTION19(chain D and resid 3:90)
20X-RAY DIFFRACTION20(chain D and resid 91:182)
21X-RAY DIFFRACTION21(chain D and resid 183:208)
22X-RAY DIFFRACTION22(chain D and resid 209:301)
23X-RAY DIFFRACTION23(chain D and resid 302:373)
24X-RAY DIFFRACTION24(chain D and resid 374:511)
25X-RAY DIFFRACTION25(chain E and resid 2:90)
26X-RAY DIFFRACTION26(chain E and resid 91:180)
27X-RAY DIFFRACTION27(chain E and resid 181:199)
28X-RAY DIFFRACTION28(chain E and resid 200:284)
29X-RAY DIFFRACTION29(chain E and resid 285:474)
30X-RAY DIFFRACTION30(chain E and resid 475:511)
31X-RAY DIFFRACTION31(chain F and resid 2:90)
32X-RAY DIFFRACTION32(chain F and resid 91:180)
33X-RAY DIFFRACTION33(chain F and resid 181:208)
34X-RAY DIFFRACTION34(chain F and resid 209:302)
35X-RAY DIFFRACTION35(chain F and resid 303:387)
36X-RAY DIFFRACTION36(chain F and resid 388:511)
37X-RAY DIFFRACTION37(chain G and resid 4:89)
38X-RAY DIFFRACTION38(chain G and resid 90:180)
39X-RAY DIFFRACTION39(chain G and resid 181:208)
40X-RAY DIFFRACTION40(chain G and resid 209:302)
41X-RAY DIFFRACTION41(chain G and resid 303:363)
42X-RAY DIFFRACTION42(chain G and resid 364:511)
43X-RAY DIFFRACTION43(chain H and resid 3:90)
44X-RAY DIFFRACTION44(chain H and resid 91:182)
45X-RAY DIFFRACTION45(chain H and resid 183:208)
46X-RAY DIFFRACTION46(chain H and resid 209:301)
47X-RAY DIFFRACTION47(chain H and resid 302:373)
48X-RAY DIFFRACTION48(chain H and resid 374:511)

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