+Open data
-Basic information
Entry | Database: PDB / ID: 3tj0 | ||||||
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Title | Crystal Structure of Influenza B Virus Nucleoprotein | ||||||
Components | Nucleoprotein | ||||||
Keywords | RNA BINDING PROTEIN / RNA-binding / Homo-oligomerization / Transcription regulation / Nucleus | ||||||
Function / homology | Function and homology information helical viral capsid / viral penetration into host nucleus / viral nucleocapsid / symbiont entry into host cell / host cell nucleus / structural molecule activity / RNA binding Similarity search - Function | ||||||
Biological species | Influenza B virus | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.233 Å | ||||||
Authors | Ng, A.K.L. / Zhang, H. / Liu, J. / Au, S.W.N. / Wang, J. / Shaw, P.C. | ||||||
Citation | Journal: J.Virol. / Year: 2012 Title: Structural basis for RNA binding and homo-oligomer formation by influenza B virus nucleoprotein Authors: Ng, A.K.L. / Lam, M.K.H. / Zhang, H. / Liu, J. / Au, S.W.N. / Chan, P.K.S. / Wang, J. / Shaw, P.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3tj0.cif.gz | 183.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3tj0.ent.gz | 145.7 KB | Display | PDB format |
PDBx/mmJSON format | 3tj0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/tj/3tj0 ftp://data.pdbj.org/pub/pdb/validation_reports/tj/3tj0 | HTTPS FTP |
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-Related structure data
Related structure data | 2q06S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 61882.129 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Influenza B virus / Strain: B/Managua/4577.01/2008 / Gene: NP / Production host: Escherichia coli (E. coli) / References: UniProt: C4LQ26 Sequence details | THIS SEQUENCE IS THE NATURAL POLYMORPHI | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.64 Å3/Da / Density % sol: 53.39 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 1.4M sodium-potassium phosphate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL17U / Wavelength: 0.97941 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: May 18, 2011 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97941 Å / Relative weight: 1 |
Reflection | Resolution: 3.2→50 Å / Num. all: 21367 / Num. obs: 19883 / % possible obs: 93.1 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 |
Reflection shell | Resolution: 3.23→3.35 Å / Redundancy: 7.3 % / Rmerge(I) obs: 0.387 / Mean I/σ(I) obs: 2.9 / Num. unique all: 1998 / % possible all: 95.1 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2Q06 Resolution: 3.233→47.055 Å / SU ML: 0.41 / σ(F): 0 / Phase error: 31.88 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.16 Å / VDW probe radii: 0.5 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 47.073 Å2 / ksol: 0.343 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 3.233→47.055 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 7
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