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Yorodumi- PDB-3t7g: Atg8 transfer from Atg7 to Atg3: a distinctive E1-E2 architecture... -
+Open data
-Basic information
Entry | Database: PDB / ID: 3t7g | ||||||
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Title | Atg8 transfer from Atg7 to Atg3: a distinctive E1-E2 architecture and mechanism in the autophagy pathway | ||||||
Components |
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Keywords | LIGASE / Atg7 / Atg3 / autophagy / E1 | ||||||
Function / homology | Function and homology information Atg12 activating enzyme activity / Atg8 activating enzyme activity / Atg8-family ligase activity / phagophore / extrinsic component of phagophore assembly site membrane / protein modification by small protein conjugation / C-terminal protein lipidation / Macroautophagy / cytoplasm to vacuole targeting by the Cvt pathway / nucleophagy ...Atg12 activating enzyme activity / Atg8 activating enzyme activity / Atg8-family ligase activity / phagophore / extrinsic component of phagophore assembly site membrane / protein modification by small protein conjugation / C-terminal protein lipidation / Macroautophagy / cytoplasm to vacuole targeting by the Cvt pathway / nucleophagy / autophagy of mitochondrion / piecemeal microautophagy of the nucleus / cellular response to nitrogen starvation / phagophore assembly site / protein targeting to membrane / autophagosome assembly / Neutrophil degranulation / macroautophagy / autophagy / protein transport / mitochondrion / membrane / identical protein binding / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.08 Å | ||||||
Authors | Taherbhoy, A.M. / Tait, S.W. / Kaiser, S.E. / Williams, A.H. / Deng, A. / Nourse, A. / Hammel, M. / Kurinov, I. / Rock, C.O. / Green, D.R. / Schulman, B.A. | ||||||
Citation | Journal: Mol.Cell / Year: 2011 Title: Atg8 transfer from atg7 to atg3: a distinctive e1-e2 architecture and mechanism in the autophagy pathway. Authors: Taherbhoy, A.M. / Tait, S.W. / Kaiser, S.E. / Williams, A.H. / Deng, A. / Nourse, A. / Hammel, M. / Kurinov, I. / Rock, C.O. / Green, D.R. / Schulman, B.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3t7g.cif.gz | 248.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3t7g.ent.gz | 201.7 KB | Display | PDB format |
PDBx/mmJSON format | 3t7g.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/t7/3t7g ftp://data.pdbj.org/pub/pdb/validation_reports/t7/3t7g | HTTPS FTP |
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-Related structure data
Related structure data | 3t7eC 3t7fSC 3t7hC C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 33422.285 Da / Num. of mol.: 2 / Fragment: NTD, UNP residues 1-289 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: ATG7, APG7, CVT2, YHR171W / Production host: Escherichia coli (E. coli) / References: UniProt: P38862 #2: Protein/peptide | Mass: 2036.193 Da / Num. of mol.: 2 / Fragment: FR, UNP residues 128-144 / Source method: obtained synthetically / Source: (synth.) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P40344 #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.51 Å3/Da / Density % sol: 64.92 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 5.5 Details: 1.8M ammonium sulfate, 0.1M Bis-Tris pH 5.5, 5mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
-Data collection
Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 8.2.2 / Wavelength: 1 Å |
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Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Dec 20, 2009 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.08→50 Å / Num. obs: 58224 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3T7F Resolution: 2.08→33.212 Å / SU ML: 0.53 / σ(F): 1.34 / Phase error: 20.61 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 53.027 Å2 / ksol: 0.35 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.08→33.212 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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